Animals ; Animals, Newborn ; Cell Survival ; drug effects ; Cells, Cultured ; Cerebral Cortex ; cytology ; drug effects ; Dose-Response Relationship, Drug ; Neurites ; drug effects ; Neurons ; cytology ; drug effects ; Organometallic Compounds ; toxicity ; Rats ; Rats, Wistar

Objective To investigate the impact of umbilical cord around the neck(UCAN) on infants developmental quotient. Methods Intelligence of 60 infants of UCAN under 3 years old were tested by Gesell. Results DQ of UCAN infants was significantly less than normal infants,mainly mild to moderate retardation. DQ between the male and female infants had no significant difference, parents' cognitive level had significant difference with real DQ of infants. Conclusions UCAN has certain influence on mental development of infants,we should strengthen health education,pay close attention to UCAN infants,give regular intelligence surveillance, find early growth retardation,give rehabilitation training to reduce the incidence of intellectual disabilities.

Adrenocorticotropic Hormone ; blood ; Animals ; Animals, Newborn ; Cold Temperature ; Environmental Exposure ; adverse effects ; Hydrocortisone ; blood ; Interleukin-10 ; blood ; Interleukin-4 ; blood ; Swine

Objective The study aimed to improve the medication safety of aged patients through the reengineering of the dispense process of discharge medications for elderly cardiac patients.Methods The original dispense process was analyzed to find the existing problems,changing the order of process,increasing or decomposing steps of process in order to redesign,perfect and implement the distribution process of medications.Time and correction rate of drug dispensing for three times a day,mastering degree of related knowledge of oral medication and satisfaction degree with medication instruction were compared before and after reengineering of the process.Results Compared with the original process,patients' ability to understand the drug knowledge,the timing when the patients take three medications per day and the satisfaction degree with the instructions of taking medications all had statistical significance.Conclusions Reengineering of the dispense process of discharge medications for elderly cardiac patients can help to improve patients' understanding of drug knowledge,improve the security of taking medications outside the hospital as well as their satisfaction degree with nursing.

Objective To clone and express the E2 gene in envelope region of hepatitis C virus and detect the anti E2 in sera of patients with HCV infection,using the purified E2 Protein.Methods The gene fragment of E2 region was obtained from the sera of patients with HCV infection by random primer reverse transcription and polymerase chain reaction(PCR),and then cloned into procaryotic expression system to express and Purify the protein and detect its antigenicity.Results A segment of E2 region gene with correct code reading frame was obtained,its full length was 984 bp and molecular weight of expressed Protein was 38kD.The detection of the protein performed by ELISA with sera from the patients with HCV infection,testified that it had appropriate antigenicity.Conclusion This experiment can provide the fundamental data for researching on the basic characteristics of HCV envelope protein,nuderstanding the significance of serological diagnosis and designing the anti HCV vassine.

Objective To explore the relationship between polymorphisms of brain-derived neurotrophic factor(BDNF) gene (rs6265 and rs12273539) and cognitive impairment in depressive disorder.Methods All participants including 73 depressed patients and 71 healthy controls were received clinical and cognitive assessments at admission,and then the depression group was divided into two groups by the score of Beijing version of the Montreal Cognitive Assessment (MoCA-BJ).One was depression with cognitive dysfunction group,and the other was depression without cognitive dysfunction group,with 36 and 37 cases respectively.The polymorphisms of BDNF gene was identified by PCR-RFLP.Results No significant difference for rs6265 gene types(x2=5.18,P=0.27),A allele carries (x2 =4.28,P=0.12) and G allele carries (x2 =1.95,P=0.38) among the three groups.There was no significant difference for rs12273539 gene types,allele carries between patients without cognitive dysfunction and controls groups(P＞0.05).There was much more C-allele carries (x2=5.40,P=0.02)and less T-allele(x2=6.06,P=0.01) in patients with cognitive disorder than those in health and it was different in rs12273539 gene types between the two groups(x2=8.38,P=0.02).CC/CT/CT gene type performed different on attention function (P＜0.01).Conclusion BDNF rsl2273539(T/C) gene type has relationship with the onset of cognitive disorder in depressed patients,and there are more C-allele carries in depressive patients.The depression patients with CC gene type are worse on the attention function impairement.

Objective To investigate the characteristics of cognitive dysfunction in patients with depression,and identify the correlation between cognitive dysfunction and serum brain-derived neurotrophic factor (BDNF) level.Methods All participants including 73 depressed patients and 71 healthy controls were received clinical and cognitive assessments at admission,the depression group was divided into two groups by the score of Beijing version of the Montreal Cognitive Assessment (MoCA-BJ),one was depression with cognitive dysfunction group which had 36 cases,the other was depression without cognitive dysfunction group which had 37 cases.Concentration of BDNF was measured by the ELISA method.Results Cognitive impairments were found in numerous cognitive domains of depressed patients,including visuospatial and executive abilities,attention,delayed recall and orientation(P ＜ 0.05).The incidence of cognitive dysfunction in depression was 49.3％.There was not significant difference between two depressive groups (depression with cognitive disorder(12.08 ± 7.08)ng/ml,depression without cognitive disorder (12.22 ± 7.93)ng/ml,P ＞ 0.05),and the levels were significantly lower than that in healthy people ((16.55 ± 7.47) ng/ml,P＜ 0.01),and serum BDNF level had no positively relevant with different cognitive functions (P ＞ 0.05).Conclusion Depression patients have cognitive dysfunction in numerous cognitive domains,including visuospatial and executive abilities,attention,delayed recall and orientation.Serum BDNF level is closely related with depression,while,it has no obvious relationship with cognition function in depression.

Objective To explore the relationship of attentional function,coping style and depressive symptoms in depression disorders.Methods Sixty-eight depression disorder and seventy-one normal healthy people were assessed by Hamilton depression scale(HAMD),coping style questionnaire and digit span forward.Independent samples t test and hierarchical multiple linear regression analysis were used to analyze all data.Resuits Compared with the control group,depressed patients had higher blaming(0.57 ± 0.29 vs 0.29 ± 0.25,P ＜0.01),wishful thinking(0.55 ± 0.22 vs 0.42 ± 0.26,P ＜ 0.01) and avoidant (0.63 ± 0.18 vs 0.46 ± 0.21,P ＜0.0l),lower problem solving (0.67 ± 0.23 vs 0.80 ± 0.18,P ＜ 0.01),rationalization (0.51 ± 0.20 vs 0.59 ±0.06,P＜0.01) and poorer attentional function(6.90 ± 1.65 vs 7.54 ±0.98,P＜0.01) ;but there was no significant differences in support seeking score.Hierarchical multiple linear regression analysis revealed that,even when controlling for age,sex,low score of support seeking were independently predicted attentional function impairment (β =-0.25,P＜0.05).In addition,the use of support seeking was found to mediate the relationship between digit span forward and retardation completely.Conclusion Retardation indirectly influence the attention function by the mediating of support seeking.

Objective: To explore the mechanisms of emodin in protecting intestinal mucosal barrier in rat with severe acute pancreatitis (SAP). Methods: Sixty SD rats were randomly divided into three groups: sham-operation group, untreated group, and emodin group. SAP in rats of the untreated group and the emodin group was induced by retrograde pumping of 3.0% sodium cholate to the common bile duct. Specimens were obtained 24 hours after the severe acute pancreatitis was induced. Serum level of leptin, serum activity of amylase and plasma content of endotoxin were measured. Ileum mucosa from ileocecal junction was observed by light microscopy and electron microscopy to measure pathological and ultrastructural changes. Apoptosis of ileum mucosal cells was detected by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling method, and expression of Bax in ileum mucosal cells was measured by immunohistochemical method. Results: Compared with the sham-operation group, there was significant increase in the levels of leptin, endotoxin, the activity of amylase, apoptosis index and Bax expression in the untreated group (P<0.01). Compared with the untreated group, the level of endotoxin, apoptotic index and Bax expression level in the emodin group were significantly reduced (P<0.01) and the leptin level was increased (P<0.05). More severe pathological changes appeared in the untreated group than in the sham-operation group under the light and electron microscopes; meanwhile less severe damage was observed in the emodin group as compared with the untreated group. Conclusion: Emodin can inhibit the apoptosis of intestinal mucosa cells and up-regulate the serum leptin content to protect the intestina1 barrier function and prevent the translocation of bacteria and endotoxin.

In this study, the effects of blood glucose and blood pressure on the compliance of large and small arteries were investigated. The results showed that arterial compliances of both large and small arteries were decreased in patients with diabetes mellitus. In non-diabetic patients with well controlled blood pressure, the compliance of small arteries was markedly improved. These results suggested that both blood glucose and blood pressure affected arterial compliance.