BACKGROUND:Internal fixation is the main treatment of humeral shaft fractures, especial y for cases accompanied several injuries. According to the fracture reduction method and fixation methods, the commonly used repair methods include intramedul ary nail and minimal y invasive plate. How to choose a safe and effective method of internal fixation is a hot issue in the repair of humeral shaft fractures. OBJECTIVE:To explore the clinical effect of different fixations of intramedul ary nail and plate in the treatment of adult humeral shaft fracture. METHODS:A total of 252 patients with humeral shaft fracture recruited from Heze Municipal Hospital from May 2010 to May 2013 were randomly divided into two groups, with 126 cases in each group. Patients in the two groups were treated with intramedul ary nail fixation and plate fixation, respectively. The average operation time, intraoperative blood loss, hospital stays, and healing time of fracture in the two groups were recorded. Al patients were fol owed up for 1 year postoperatively, the incidence of radial nerve injury, infection and non-union was compared. The recovery of shoulder joint and elbow joint of patients was assessed with Neer method and Mayo method, respectively. RESULTS AND CONCLUSION:After treatment, the rate of primary success of internal fixation showed no significant differences between the two groups (χ2=0.399 7, P>0.05). The average operation time, intraoperative blood loss, the healing time of fracture and hospital stays in the intramedul ary nail group were significantly shorter than that in the plate group B, the difference of the results were extremely significant in two groups (t=15.442 5, 21.713 4, 8.217 6, 1.214 8, P<0.01). After 1-year fol ow-up, the incidence of radial nerve injury showed extremely significant differences between the two groups (χ2=8.262 3, P<0.01);the incidence of nonunion in the intramedul ary nail group was significantly lower than that in the plate group, with extremely significant differences (χ2=3.947 2, P<0.05). The overal incidence of complications in intramedul ary nail group was significantly lower than that in the plate group, with extremely significant difference (χ2=11.977 9, P<0.01). Besides, the Neer scores of shoulder joint and the Mayo scores of elbow joint between the two groups had no statistical y significant difference (χ2=0.235 4, 0.273 0, P>0.05). Both intramedul ary nail and plate fixations show good clinical effect in treatment of adult humeral shaft fracture, and each technique has their advantages and disadvantages, we should choose the proper technique according to the patient’s condition.

Objective:To establish a recombinant adenovirus vector carrying HBX gene for investigation of the functions of HBX gene on the hepatocellular carcinoma.Methods:HBX gene was amplified from genomic DNA of HBV by polymerase chain reaction(PCR) technique, and subcloned to plasmid pHAHA to construct recombinant plasmid pHAHA-HBX.subcloned HAHA-HBX into a shuttle vector pAdTrack-CMV.After being identified by endonuclease,and subsequently cotransformed into E.coli.293 cells with an adenoviral backbone plasmid,e.g.pAdEasy-1.Results:HBX gene was cloned from HBV genome by PCR successfully,and proved by sequence determination.A vector producing cell line 293/HBX was established.An HBX gene integration was detected by PCR in the recombinant adenovirus vectors.Conclusion:A recombinant adenovirus vector containing HBX gene was successfully constructed.

Objective:To develop a Wnt-dependent ?-catenin-mediated functional assay system.Methods:To construct a vector,pCMV-bcGFP,which can express the ?-catenin and green fluorescent protein(GFP) fusion protein;transfect HEK293 with the vector,select the cells with G418 and LiCl and establish the stable cell line,293-bc-GFP by cloning.Results:293-bc-GFP stable cell line was developed;the background expression of ?-catenin-GFP fusion protein was detectable at a very low level in the cell line under fluorescent microscope and via Western blotting analysis;however,the fusion protein was showed to significantly increase in the presence of Wnt1 and lithium.Conclusion:Our results showed that the system is regulated by ?-catenin in a Wnt-responsive fasion.Thus,it can be used as a functional reporter to identify potential upstream factors during tumorigenesis,as well as to screen for the potential anti-cancer agents that specifically inhibit ?-catenin signaling in human tumors.

The practice guidelines on Alzheimer's disease and other types of dementia have been developed and revised by European federation of neurological societies(EFNS),American academy of neurology(AAN) and American psychiatric association(APA) recent years.The aim of these international guidelines was to present evidence-based statement for the care of patients with dementia.These guidelines on dementia cover major aspects of diagnosis,with particular emphasis on assessment of cognitive functions and psychological symptoms,the blood tests,neuroimaging,electroencephalography(EEG),cerebrospinal fluid(CSF) analysis,genetic testing and tissue biopsy.

OBJECTIVE: To evaluate characteristics and security of artificial synthetic material for repairing incisional hernia of abdominal wall. METHODS: Articles about randomized controlled studies which were focus on repairing incisional hernia of abdominal wall were collected from CNKI from 2001 to 2009 with the retrieval words of "repairing materials for hernia,polypropylene patch, and incisional hernia ofabdominal wall". And then, those articles were summed up and analyzed. RESULTS: Among 24 tests, a total of 1 393 patients who had incisional hernia of abdominal wall, abdominal-wall defect, andinguinal hernia were included in the final analysis. Polypropylene patch and polytetrafluoroethylene patch could reconstruct integrity of abdominal wall during repairing large and huge incisional hernia, characterizing by less trauma, simple operation, rapid recovery, and low relapse rate. However, import patches were expensive. Home-made dacron materials for repairing hernia has been compared with import polypropylene patch, and the results indicated that dacron materials had light foreign body sensation and good complaisance after implantation; additionally, there was no significant difference in security and adverse reaction between dacron materials and import patches. CONCLUSION: Artificial synthetic materials characterize by great biocompatibility, strong anti-tensile strength, excellent tolerance to inflexion, optional cutting, beneficial for abdominal-wall defect at differential sizes, no comfortlessness, rapid recovery, low relapse rate, and less complication. Thus, artificial synthetic materials are extensive prospect.

Objective To investigate the effect of rehabilitation education of movement disorders of Parkinson's disease in general hospital. Methods The postgraduates and visited physicians in department of neurology received curriculum of rehabilitation for movement disorders. They were investigated with the questionnaire after curriculum. Results All the postgraduates and visited physicians accepted the role of rehabilitation for movement disorders after the curriculum (P<0.001), and learnt some knowledge about the rehabilitation. Most postgraduates (37/62) preferred teaching based on clinical cases, while the visited physicians (49/60) preferred demonstration. For the content, most the postgraduates (35/62) preferred academic progress, and the visited physician (50/60) preferred operation and practice. Conclusion It is necessary and feasible to teach rehabilitation for movement disorders in neurology of general hospital.

The important difference between post-graduate and undergraduate education is to train their reseach ability,which is very important especially to the post-graduate students of basic medicine who will be researchers.The problem in training research ability of post-graduate was studied in this paper and solution plan was put forward.

Objective To investigate the features of magnetic resonance imaging(MRI)in the brain of patients with multiple system atrophy(MSA)and compare with that in Parkinson Disease(PD).Methods Thirteen patients with MSA-C,12 patients with MSA-P,and 30 patients with Pakinson disease(PD)were retrospectively studied in terms of MRI data.Results The area of the pons in the patients with MSA-C(386.4±74.2)mm2 was significantly smaller than in those with PD(528.5±40.8)mm2,as well as the average of the pons transverse diameter([17.9±1.8]mm in the patients with MSA-C and[20.2±1.4 lnun in PD patients)(t=6.5 and 4.7,Ps<0.01).The area of the pons in MSA-P patients(474.1±88.0)mm2 and the average of the pons tranverse diameter(19.3±2.4)mm were similar to those of PD patients(Ps>0.05).The average width of middle cerebellar pedncles(MCP)in MSA-C(6.1±1.6)mm and MSA-P(6.5±1.2)mm were smaller than those in PD patients(9.1±1.0)mm(t=6.2 and 7.1,Ps<0.01),whereas there was no significant difference between MSA-C and MSA-P group(P>0.05).In the MRI findings,cerebellar atrophy was the highest sensitive,and cruciform hyperintensity and hyperintensity of MCP followed.The MBI profiles were more specific for hyperintensity of cruciform and MCP than for cerebellar atrophy in MSA-C population.For MSA-P population,MRI was relatively sensitive for hyperintensity of rim of the putamen and putamen,but less specific.Conclusion The assessment of MRI may be useful to diagnose and distinguish MSA from PD.

[ ABSTRACT] AIM:To investigate the effect of sodium valproate ( VPA) on bleomycin-induced pulmonary fibro-sis ( PF) and its mechanism.METHODS: SD rats ( n =42 ) were randomly divided into model group and treatment group.Bleomycin at dose of 5 mg/kg was intratracheally injected to establish a rat PF model.The rats in treatment group were given normal saline (NS, 0.5 mL/d), VPA (300 mg· kg-1· d-1) or dexamethasone (DEX, 0.6 mg· kg-1· d-1 ) via intraperitoneal injection from the 14th day to the 28th day after modeling.The rats in model group were sacrificed on 0 d, 14 day and 28 d after modeling .The rats in treatment group were killed at 14th day after treatment.The effects of VPA on PF were evaluated by HE and Masson staining.The hydroxyproline ( HYP) content in the rat lung tissues was de-tected, and the expression ofα-smooth muscle actin (α-SMA) and E-cadherin was determined by Western blotting.RE-SULTS:HE staining showed that the alveolar structure and interstitial morphology in VPA group were better than those in NS group and DEX group.The level of collagen in VPA group was significantly lower than that in DEX group and NS group by determining the HYP content and Masson staining.VPA reduced the expression ofα-SMA, a mesenchymal marker pro-tein of PF, while increased the expression of epithelial marker protein E-cadherin.CONCLUSION:VPA reduces collagen content and distribution, and up-regulates the expression of the epithelial marker protein E-cadherin, while down-regulates mesenchymal marker proteinα-SMA, thereby preventing the rat lung tissues from bleomycin-induced PF.

Objective To explore the efficacy of shugan -jieyu capsule and escitalopram in treating post -stroke depression (PSD).Methods 80 cases with PSD were randomly divided into the study group(administered with shugan -jieyu capsule and escitalopram)and the control group(administered only with escitalopram),40 cases in each group.Hamilt Depression Rating Scale(HAMD),Modified Einberg -Scandinavian Stroke Scale(MESSS)and Treatment Emergent Symptoms Scale(TESS)were used to assess treatment effect before treatment and at the weekend of 2nd,4th,8th after treatment.Results The scores of HAMD and MESSS in the study group were much lower than those in the control group.The scores of HAMD at 4th weekend and 8th weekend in the study group were (16.92 ± 3.75),(8.60 ±3.25),those in the control group were (19.27 ±4.11),(11.13 ±3.72)(t2w =-2.671,t4w =-3.239,P <0.05).The scores of MESSS at 4th weekend and 8th weekend in the study group were (20.06 ±3.87), (12.47 ±3.97),which in the control group were (21.68 ±3.12),(15.09 ±3.20)(t2w =-2.061,t4w =-3.250, P <0.05),while the effective rate in the study group(80.0%)was significantly higher than that in the control group (45.0%)(χ2 =5.227,P <0.05).Conclusion The efficacy of shugan -jieyu capsule combined with escitalopram for PSD is superior to use escitalopram alone.