Animals ; Dental Cements ; chemistry ; Glass ; chemistry ; Humans ; Materials Testing ; Post and Core Technique ; instrumentation ; Quartz ; chemistry ; Root Canal Therapy ; Tooth Fractures ; prevention & control

Humans ; Mouth ; Photography

Color ; Dental Materials ; Dental Restoration, Permanent

Objective: To investigate the aberrant methylation of CpG island in 5′ promoter region of p16 gene in the pancreatic juice and its value in diagnosis of patients with pancreatic cancer. Methods: Pure pancreatic juice(PPJ) was collected from the pancreatic duct by a nasopancreatic tube put under endoscopic retrograde cholangiopancreatography (ERCP). Cytological examination was performed by H-E staining in pure pancreatic juice. Aberrant p16 methylation was detected using the methylation-specific PCR (MSP) in the PPJ. Results: The sensitivity, specificity, positive predictive value, negative predictive value and accuracy cytological examination in diagnosis of pancreatic cancer were 40%, 100%, 100%, 45.4% and 60.0%, respectively. The DNA sequences were successfully extracted from the PPJ of 30 patients with pancreatic diseases and were subjected to MSP. Seven(35%) of the 20 cases with pancreatic cancer showed aberrant methylation of p16 gene. No aberrant methylation was detected in the pancreatic juice samples of patients with chronic pancreatitis and mucinous cystoadenocarcinoma of pancreas. When cytological examination combined with p16 methylation detection, the sensitivity, specificity, positive predictive value, negative predictive value and accuracy for diagnosis of pancreatic cancer were 55%, 100%, 100%, 52.6% and 70%, respectively. Conclusion: Pancreatic juice collected by nasopancreatic drainage during ERCP can be used for molecular analysis. Detection of aberrant methylation of p16 gene in pancreatic juice combined with cytological examination is a better method for diagnosis of pancreatic cancer.

Objective: To observe the change of cilia movement in rabbit sinusitis model before and after sinusitis surgery. Methods: Forty New Zealand White rabbits were divided into 3 groups: normal control (35 sides), sinusitis group (50 sides), and post-surgery sinusitis group (40 sides). The experimental maxillary sinusitis model was made by bacteria injection and ostia closure. Rabbits with sinusitis were treated by opening and enlarging ostia of maxillary sinus. Activated charcoal powder and 99mTc-SC methods were used to observe the mucociliary transport (MCT) in maxillary sinus. Results: 99mTc-SC method satisfactorily reveal the direction of clia movement. Activated charcoal powder showed that the order of MCT velocity in 3 groups was: normal control group> post-operation group>pre-operation group (all P<0.01). Conclusion: Activated charcoal powder is an effective method in estimating the function of maxillary sinus cilia. The function of cilia of post-operative regenerated mucosa is lower than that of normal mucosa cilia.

Objective: Finding the essence and regular pattern of the construction of male urine collection devices, and setting up its basic concepts. Methods:Selecting typical construction examples, then analyzing them singly and as a whole. Results:Setting up concepts such as construction module, construction factor, construction factor unit, construction index, construction array etc., and giving out the table of construction factor units. Conclusion:This research has provided mode, methods, means for understanding and further analyzing, studying male urine collection devices.

ObjectiveTo analyzes the influence of psychological factors on the rehabilitation of the professional ennui (PE) of teachers in Institutions of Higher Learning (TIHL).MethodsMaslach Burnout Inventory-ES and the Symptom Checklist 90 (SCL-90) were used to investigate the subjects of TIHL. ResultsThe scores of PE showed distinct positive correlation with every factor of SCL-90 (P<0.01). ConclusionPE of TIHL is correlative with SCL-90, which seems that improvement of psychology may improve the PE of TIHL.

OBJECTIVE: The aim of this study was to investigate the association of JAK1 polymorphisms with allergic rhinitis in China Han population. METHOD: A total of 450 patients with AR and 615 healthy subjects as control were genotyped for the presence of three single nucleotide polymorphisms using polymerase chain reaction restriction fragment length polymorphism (PGR-RFLP) analysis of DNA extracted from blood samples. RESULT: All control subjects were in Hardy-Weinberg equilibrium, but high frequencies of JAK1 the homozygous rs310241 CC genotype were observed in AR patients compared to controls (P < 0.05). The results also revealed that there was no association between the rest of two investigated SNPs and AR. CONCLUSION Our results suggested that JAK1 gene rs310241 CC genotype was associated with patients with AR.
Asian Continental Ancestry Group ; genetics ; China ; Genetic Predisposition to Disease ; Genotype ; Humans ; Janus Kinase 1 ; genetics ; Polymerase Chain Reaction ; Polymorphism, Restriction Fragment Length ; Polymorphism, Single Nucleotide ; Rhinitis, Allergic ; genetics

OBJECTIVE: To determine whether chronic alcoholism alters the expression levels of Vasoactive intestinal polypeptide (VIP) and its receptor (VIPR1) in the cochlea of chronic alcoholism rats. METHOD: We measured their expression levels in 30 SD rats, in which we created models of different degrees of chronic alcoholism. We investigated the presence of the mRNA of VIP in the cochlea of chronic alcoholism rats and controls by reverse transcription-polymerase chain reaction (RT-PCR) method. We investigated the presence of proteins of VIPR1 in poisoned rats and controls by western blot. We also evaluated the local distribution of VIP cells by immunohistochemistry. RESULT: We found that the levels of VIP and VIPR1 were downregulated in the chronic alcoholism groups compared to the controls group. The differences in some expression levels were significant different between chronic alcoholism rats and control rats. Moreover, at different degrees of alcohol poisoning in rats, the contents of VIP and VIPR1 differed. Decreased levels of VIP and VIPR1 were detected in the deep chronic alcoholism group compared to the group with low-degree poisoning (P < 0.05). In spiral ganglion cell plasm the expression of VIP and VIPR1 had no significant difference in three groups (P > 0.05). CONCLUSION These results suggest that VIP and VIPR1 play an important role in the auditory function in rats with chronic alcoholism. Chronic alcoholism may cause a peptide hormone secretion imbalance in the auditory system, eventually leading to hearing loss.
Alcoholism ; metabolism ; Animals ; Cochlea ; metabolism ; Disease Models, Animal ; Down-Regulation ; RNA, Messenger ; Rats ; Rats, Sprague-Dawley ; Receptors, Vasoactive Intestinal Polypeptide, Type I ; metabolism ; Spiral Ganglion ; Vasoactive Intestinal Peptide ; metabolism