BACKGROUND:Proteomics is a new field of research in the post-genomic era. The research subjects of proteomics include the structure and function of entire proteins in tissues or cells. It analyses the vital changes of pathological and physiological process of diseases by examining proteins.
OBJECTIVE:To summarize the application of proteomics in orthopedic diseases and its prospect.
METHODS:Articles about the application of proteomics in orthopedic diseases and its prospect were retrieved from the Digital Library Al iance of Zhujiang River Delta and PubMed with the key words of“proteomics, orthopedics, review, osteoblasts metabolism, osteoclasts metabolism, intervertebral disc degeneration, osteoporosis, osteoarthritis, serum related orthopedic diseases”in both Chinese and English from January 2001 to September 2013. Reproductive research and atypical reports were excluded.
RESULTS AND CONCLUSION:Proteomics has been applied in the osteoblasts metabolism, osteoclasts metabolism, intervertebral disc degeneration, osteoporosis, osteoarthritis and serum related orthopedic diseases that are the most common il nesses in al types of people. These basic and clinical studies undoubtedly bring great benefit for human health, and proteomics technology wil get a bigger breakthrough in basic medicine and clinical medicine, and it shows us a broad prospect.

Objective To explore the characteristics and trends of adaptive behavior among patients with breast cancer during the treatment periods. Methods Phenomenological research method was used. 15 women accepted modified radical mastectomy in the Provincial Hospital of Anhui Medical University were interviewed during the whole treatment periods. Results 6 adaptive behavior models were found: over-cautiousness, compliance, escape-avoidance, specific social circles, social re-adaptation and dietary behavior change. These six behavior models dynamicly varied during the treatment trajectory. Conclusions Diagnosis and treatment of breast cancer are strong negative life events, the unalterable trauma of body and the periodic shocks by chemotherapy force patients' self-positioning shifting between "wellness"and "illness"and this shift is the main reason that caused changes of behaviors.

Objective Diagnosis of GDM during the early period and treatment in time can improve motherchild prognosis.Methods 805 pregnant women with gestational age of 24～28 weeks were screened by the 50g 1hour glucose challenge test(GET).75g oral glrcose tolerance test(OGTT)were further performed in the abnormal pregnant women,and the normal or abnormal pregnant outcomes were followed.Results The morbidity of pregnant women and neonates were higher in-group of gestational impaired glucose tolerence(GIGT)and geztational diabetes mellittts(GDM)(P＜0.05 or P＜0.01).Conclusion The research suggests that all pregnant women must be screened for detecting diabetes mettitus.The abnormality of glucose tolerance test need to be further treated and monitored.

Hospital medical ethics is the basis of survival,and an important part of management in modern hospitals.Through the implementation of humanistic services,regulated medical behaviors,expeditious communication channels,and satisfying service for patients with all efforts,can modern hospitals establish a good image for themselves and gain fine reputation from patients.

Objective To assess the cost-effectiveness of different modes of the cataract operation. Methods Firstly, 291 cases of cataract inpatient were divided four groups according to operation modes (PHACO and ECCE) and the prices of intraocular lens (IOL) (high and low). Then the comparisons of inpatient cost, operation cost, postoperative VA, postoperative VF and QOL were made among four groups respectively. Finally, the cost-effectiveness of different cataract operation modes was analyzed by comparing the differences of their costs with the VA, VF and QOL improvement. Results There were significant differences in the above indicators among four groups in general (P 0.01). The VA improvement amount of PHACO was higher than that of ECCE whereas the VF and QOL improvement lower than those of ECCE. The price of IOL had no effect on the improvement quantity of VA, VF and QOL. When one unit amount of VA improvement wanted, the cost-effectiveness ratio of inpatient with PHACO and low-price IOL was the lowest. When one unit amount of VF and QOL improvement wanted, the cost-effectiveness ratio of patients with ECCE and high-price IOL was the lowest. When one unit amount of VA and QOL improvement wanted, the operation cost-effectiveness ratio of ECCE with low-price IOL was the lowest. And when one unit amount of VF improvement wanted, the operation cost-effectiveness ratio of ECCE with high-price IOL was the lowest. Conclusions There were cost differences among different cataract operation modes. The cost of PHACO was higher than that of ECCE. The ECCE mode and price of IOL were the main determinants of cost. The effects of improving VA and QOL by ECCE were better and the effect of improving VA by PHACO was better than by ECCE. The homemade or low price IOL had no influence on the cataractous operative effectiveness, but could decrease the operation cost. ECCE with low-price IOL was the optimal cost-effectiveness strategy and operation mode, especially for poor rural and remote areas.

OBJECTIVE:To provide reference for improving the data integrity management system of pharmaceutical produc-tion enterprises. METHODS:According to related reports in US Food and Drug Administration(FDA)and China Food and Drug Administration(CFDA),the source of data integrity problems was analyzed,its reasons were summarized and solutions were put forward. RESULTS&CONCLUSIONS:The reasons why there were data integrity problems in enterprises can be attributed to 3 as-pects(staff,hardware/software system and quality management),and the 3 aspects showed large gap with current standards. It is suggested that enterprises evaluate the existing system by adopting the gap analysis,establish a data integrity management project team,strengthen personnel training,upgrade hardware/software system to ensure its safety,stability and effectiveness;and opti-mize the quality management system by developing good ducument specification,special regulation system of data integrity. In addi-tion,the enterprise should establish quality culture,pay attention to industry and regulatory trends in real time to guarantee the data integrity effectively.

Objective: To establish a method for the determination of amidozon and amantadine hydrochloride in compound amantadine hydrochloride tablets by HPLC-ELSD.Methods: The analytical column was an Agilent Eclipse Plus C18 column (250 mm×4.6 mm, 5 μm).The mobile phase consisted of 0.1% trifluoroacetic acid solution-acetonitrile (85∶15) and the flow rate was 0.7 ml · min-1.The column temperature was room temperature.Both evaporation temperature and atomization temperature were 40 ℃.The flow rate of carrier gas (nitrogen) was 1.8 L · min-1 and the injection volume was 10 μl.Results: The peak area and logarithm of injection volume of ampicrine showed a good linear relationship within the range of 606.72-3 033.60 μg · ml-1 (r=0.999 7), and that of amantadine hydrochloride showed a good linear relationship within the range of 400.96-2 004.80 μg·ml-1 (r=0.999 4).The average recovery was 99.5% and 99.3%, and RSDs was 0.35% and 0.40%(n=9), respectively.Conclusion: The method is simple, rapid, accurate and reproducible, and suitable for the determination of aminopyrine and amantadine hydrochloride in compound amantadine aminopyrine tablets.

AIM: To establish the quality standard for Xiangpu Ganmao Capsule (Herba Moslae, Cortex Magnoliale Officinalis, Radix Scutellariae). METHODS: Herba Moslae, Cortex Magnoliae Officinalis and Radix Scutellariale of Xiangpu Ganmao Capsule were identified by TLC. Thymol, magnolol and honokiol of capsule were determined by HPLC together. RESULTS: The characteristic identification by TLC was distict and highly specific. The quantitative evaluation of thymol had the linear range of 0.32-3.2 ?g, The average recovery was 99.44% and RSD was 1.3. The quantitative evaluation of magnolol had the linear range of 0.20-2.0 ?g. The average recovery was 99.78% and RSD was 1.58. The quantitative evaluation of honokiol had the linear range of 0.49-(4.89 ?g.) The average recovery was 99.41% and RSD was 0.51. CONCLUSION: The method is reliable, accurate and specific. It can be used for quality control of Xiangpu Ganmao Capsule.

Catheter Ablation ; Humans ; Thyroid Nodule ; surgery

Apurinic/apyrimidinic endonuclease/redox factor 1(APE/Ref 1) is a multifunctional protein that is responsible for repairing DNA damage,it also infleunce reduction oxidation (redox) reaction and modulate the DNA binding activity of transcription factors,it is important to cell survival.Recently, much data concerning APE1/Ref 1 and information on APE1/Ref 1 in a wide variety of functions and systems have rapid growth,including its gene,function,distribution and relations with some diseases,especially nervous system diseases and tumors.