MAPK(mitogen-activated protein kinase) family,which is conserved throughout high eukaryotes,is implicated in multiple cellular processes including cell growth,migration,proliferation,differentiation,survival and development.Pathogen hijacks hosts' MAPK pathways to facilitate its pathogenesis using diverse strategies.To further explore the mechanism underlying interactions between pathogens and hosts' MAPK pathway,is of benefit to our understanding of nature as well as to our fight against pathogen infection.

Objective To evaluate the efficacy of endovascular interventional minimally invasive techniques in treatment of acute brachial artery injury.Methods A retrospective case series study was designed to analyzed data of 16 cases (11 males and 5 females) sustaining acute brachial artery injury treated from March 2011 to August 2016.Mean age was 47.9 years (range,27-66 years).Left upper extremity vascular injury occurred in 7 cases and right upper extremity vascular injury occurred in 9.There were 13 cases of arterial intima injury and 3 of arterial pseudoaneurysm.All patients underwent endovascular therapy including stent implantation and balloon dilatation.Angiography was used to evaluate the repair of brachial artery and the operation time was recorded.Improvements of distal limb arterial pulse and limb ischemic symptoms were observed,and the patients who were followed up with stent lumen stenosis more than 50％ received endovascular treatment again.Results The procedures was successful in all patients,with operation time of 30-75 minutes (mean,45 minutes).After the insertion of a stent,recovered brachial artery blood flow and disappeared pseudoaneurysm were observed on angiography,without occurrence of stent stenosis or contrast extravasation.Symptoms of local pain and numbness were alleviated,and blood supply of the distal brachial artery was improved after operation.All patients were followed up for 3-36 months(mean,16.7 months).The false aneurysm did not recur.Two cases of stent stenosis more than 50％ had a second balloon dilation procedure and the blood flow returned to normal.Stent patency was found in 14 cases with stent stenosis of less than 50％.During the follow-up period,no ischemic necrosis,displacement of the stent or stent fracture were found.Conclusion For acute brachial artery injury,endovascular management exhibits higher technical success rate and good clinical outcome with a low incidence of complications.

Adolescent ; Adult ; Female ; Histiocytic Necrotizing Lymphadenitis ; diagnosis ; therapy ; Humans ; Lymph Nodes ; Male ; Middle Aged ; Neck ; Young Adult

Objective To investigate the application status of aspirin for secondary prevention among elderly patients with ischemic stroke in some community hospitals in Shanghai,and explore the main causes for inappropriate use of aspirin and the effect of aspirin on the prevention of ischemic stroke recurrence. Methods The cross-sectional investigation was employed,and 223 patients from 10 community hospitals of Shanghai were divided into regularly-received aspirin group(n=98) and irregularly-received aspirin group(n=125).The application status of aspirin was investigated and the relationship between aspirin application and ischemic stroke recurrence was explored. Results Fifty-one cases in irregularly-received aspirin group(40.80%) stopped aspirin use or reduced the dose due to possible adverse effects,which accounted for 22.87% of the total population investigated,and 42 cases in irregularly-received aspirin group(33.60%)were never suggested to use aspirin or only used traditional Chinese medicine.The rate of ischemic stroke recurrence was 30.61%(30 cases) in regularly-received aspirin group,and 49.60%(62 cases) in irregularly-received aspirin group,which existed significant differences between these two group(P

Objective:To investigate the status of humanistic quality in nursing undergraduates interns , to pro-vide a reference for optimization settings humanities courses and improving humanistic quality in nursing students . Methods:A self -designed questionnaire with convenience sampling method was used to investigate 90 nursing students in M hospital .The questionnaires included requirements for clinical teachers , needs for humanities cour-ses, cognitive knowledge on humanities curriculum .Results:The nursing intern students agreed with teachers're-spect for students , profession ability , attention to communication .They wanted to increase the humanities course of interpersonal and communication , nursing aesthetics and rituals , traditional Chinese culture courses .They had a humanistic understanding of the concept of learning is to help develop good mental state and healthy personality , enhance the sense of community , strengthen exchanges and cooperation with the person's ability .Conclusion:The nursing intern students have recognized the importance of humanities courses .Increasing humanities curriculum and using a combination of diverse teaching and assessment methods were proposed to promote their humanities quality of ability.

It is very important to train junior doctors'clinical skills,especially surgical skills.Operation protocol presentations before each surgical procedure provide good opportunities for them to learn these skills,and at same time,this presentation ensures a better surgical outcome as well.Each presentation is prepared under the direction of a senior doctor.A protocol is composed of the patient medical history,physical examination,accessory check up reports,diagnosis and surgical procedure.It is not only a good method for the young doctors to learn but also for their teachers to teach each student individually.

The nutritive composition from tissues of Perinereisaibuhitensis was analyzed. The result indicated that there was 67. 43% amino acids and 14. 24% fatty acids in the tissues, a-mong them the EPA comprises 6. 5343% of the total fatty acids and the glutamic acid comprises 10. 288% of the whole tissues. In the meanwhile, the tissues also contains high content of trace elements, such as zinc, iodine, selenium as well as fibrinolytic enzymes and so on, showing a prospective application value in the future.

Active fractions were obtained from the c(?)amworm homogenate by reverse-phase concentration, gel filtration and affinity column chromatography. The MTS/PMS assay was used in the screening of antimicrobial peptide in vitro. The MTS/PMS assay and ELISAAFP method were combined to evaluate the effects of antimicrobial peptide on the proliferation and ?-fetoprotein secretion of HepG2 cells as well as on the proliferation of normal mouse osteoblast MC3T3-E1. The results showed that the purified antimicrobial peptide from clamworm Murphysa sanguinea was a constitutive basic protein with MW 8.1 kDa and pI 8.6, which showed inhibition effects on the proliferation and ?-fetoprotein secretion of HepG2 cells at different levels. These effects have a positive correlation with the concentrations of antimicrobial peptide. No obvious inhibition and damage effects were observed on the normal osteoblast.

Objective To evaluate the safety and efficacy of homemade patent ductus arteriosus (PDA) closure device for patients with very large PDA. Methods A total of 15 patients (12 females) with very large PDA, underwent transcatheter occlusion with homemade PDA devices. The median age was 21?8 (16-46) years old. The median diameter of the PDA at its narrowest point was 16?3 (13-22) mm. Aortic angiography and measurement of pulmonary pressure were performed before and after the closure of PDA. Repeat echocardiography was performed on each patient at one week, one month and six months after the procedure. Results Fifteen patients underwent transcatheter closure using a device of 23?4 (18-32)mm in diameter, and all of them showed a trace to small shunt by aortic angiography 30 minutes after the procedure. Thirteen patients was successfully occluded with a peak systolic pulmonary pressure decreased from 113?21 mm?Hg to 70?29 mm?Hg (P

Objective To explore the status quo of the career planning of the second child nursery under the two-child fertility policy, and to provide reference for carrying out the targeted management intervention. Method From April to May of 2017, 592 nurses of the second child were surveyed using the general data questionnaire and the career planning scale. Results The nurses' career planning score (34.81 ± 3.59) was different, and the career planning of the two nurses with different age, job title, title, establishment, department and income and fertility was different and statistically significant. Nurses with plan to a second child are with less desire to further study and promotion than those without plan to a second child (all P<0.001) promotion to open more than two children before the low policy (P <0.05). Conclusion Nurses' career planning for births is on a middle level. Nursing managers should pay attention to the management of nurses' career planning , create a career development environment , provide humanistic care , strengthen self-awareness and career self-development of nurses who have two births , so as to improve the level of their career planning to promote the nursing team development.