ObjectiveTo survey the usage of prophylactic antimicrobial agents in type Ⅰ incision surgical operation.MethodsThe data of medication of 140 patients with type Ⅰ incision operation including thyroid surgery,breast surgery and repairing hernia surgery were investigated and statistically analyzed.ResultsThe preventative antibiotics usage in type Ⅰ incision operations was 100％.The frequency ranking of the perioperative antibiotics application was cephamycins ( 116 cases),cephalosporins ( 18 cases) and aminoglycosides ( 8 cases ).127 patients received antibacterials before operation.The total time for medication at least 1 day,up to 4 days.The antibacterials used in single kind and two kinds were 136 cases and 4 cases.ConclusionThe prophylactic use of antimicrobials in patients undergoing type Ⅰ incision operations in this hospital was irrational in the rate of antimicrobials use,choice of drug categories and medication duration,etc.Antibiotic prophylaxis in type Ⅰ incision perioperative should be further strengthened to supervise and management so as to promote rational use.

The preliminary results of inactivation of model parvovirus M13Mp 18 in plasma by long-waveUVA irradiation combined with psoralen derivatives were described.At 8-MOP concetration of300?g/ml plasma and UVA intensity of 11.5mW/cm~2,the UVA irradiation for 30～120 min couldresult in virus inactivation of 10~(5～9) infectious dose/ml.Quenchers were used to reduce the damage ofUVA to proteins in order to improve the clotting factor recovery after irradiation.2mmol/Lglutathione,or 2mmol/L glutathione with 2 mmol/L mannitol could significantly improve the Frecovery after irradiation.

Acupuncture Points ; Adult ; Diarrhea ; therapy ; Female ; Ginger ; chemistry ; Humans ; Irritable Bowel Syndrome ; therapy ; Male ; Middle Aged ; Moxibustion

Objective To explore the clinical process and prognosis of middle and intermediate uveitis in children and adults.Methods A total of 121 patients with intermediate uveitis admitted to our hospital from January 2012 to December 2014 were enrolled in the study.Among them,there were 67 cases with monocular involvement and 54 cases with binocular involvement;59 cases were children and 62 cases were adults.All subjects were treated with personalized regimen according to the severity of the disease.The treatment effects and prognosis were compared between patients with different ocular involvement,children and adults.Results The total effective rate of treatment in patients with monocular involvement,children,patients with binocular involvement and adults were 92.5％,93.2％,92.6％ and 91.9％,respectively (all P ＞ 0.05).There was no significant difference in acute phase protein and visual acuity between the patients with ocular involvement and children,adults before treatment (all P ＞ 0.05),and there was no significant difference in the indexes between two groups after treatment (all P ＞ 0.05).The acute phase protein in each group after treatment was decreased,and the visual acuity was obviously increased,there were statistical differences before and after treatment (all P ＜ 0.05).Conclusion Individualized treatment regimen for patients with intermediate uveitis can improve the therapeutic effect,control inflammation and improve visual acuity.The curative effect is not significantly related to age and degree of ocular involvement.

Objective To investigate the effect of melatonin on serum malondialdehyde (MDA), NF-κB, tumor necrosis factor-α(TNF-α) andβ2-microglobulin (β2-MG) levels in secondary brain damage following intracerebral hemorrhage patients.Methods 90 patients secondary brain damage following intracerebral hemorrhage were divided into experimental group and control group, 45 cases in each group.The control group were given conventional treatment after surgical removal of hematoma, and the experimental group, on the basis of control group, were given melatonin capsule (6 mg melatonin) , orally, once a day, for 2 weeks treatment.The levels of MDA, NF-κB, TNF-αand β2-MG in patients’ serum were detected.Results The MDA, NF-κB, TNF-αand β2-MG levels after treatment significantly decreased compared with before treatment in two groups (all P<0.05), and the above indexes in experimental group significantly decreased compared with control group ( P <0.05 ).Conclusion Melatonin can significantly reduce the levels of MDA, NF-κB, TNF-αand β2-MG levels in brain injury patients with cerebral hemorrhage, and repair brain damage, which has a guiding significance for clinical use.

Objective To study the effect of caveolin-1 on renal injury and the expression of tight junction proteins in MRL/lpr mice kidney.Methods The mice were divided into 4 groups:5 mice in the normal control group (BALB/c mice);the MRL/lpr lupus mice (n=18) were randomly divided into the MRL/lpr group in which 6 mice were included;the negative control group in which 6 mice were included;the caveolin1 transfection group in which 6 mice were included.The changes of urine protein,the levels of urea (BUN) and creatinine (Cr) were detected.The expressions of claudin-5,occludin,ZO-1 and caveolin-1 protein were determined by western bloting.Analysis of variance was used to determine statistical significant differences between the two groups.A significance level of 0.05 was considered as signigicant.Results Compared with the control group,24 h urine protein [(2 894±437) mg,(412±72) mg],BUN [(8.7±1.5) mmol/L,(6.9±0.4) mmol/L],Cr [(106±22) μmol/L,(85±4) μmol/L] were significantly increased,level of caveolin-1 protein increased (265±17,61±6),the level of occludin (114±12,190±12),claudin-5 (60±5,80±6) and ZO-1 (98±11,206±15) protein decreased in the MRL/lpr group (P＜0.05).After caveolin-1 transfection,the levels of urinary protein [(1 253±249) mg,(2 894±437) mg],BUN [(6.5±1.3) mmol/L,(8.7±1.5) mmol/L],Cr [(78±17) μmol/L,(106±22)μmol/L] were significantly decreased,and the levels of occludin (218±16,114±12),claudin-5 (87±6,60±5)ZO-1 (313±17,98±11) were increased (P＜0.05).Conclusion The expression of caveolin-1 protein in the renal tissues of lupus nephritis increases.Caveolin-1 can reduce the expression of tight junction proteins and contribute to progres-sion of lupus nephritis.

The Cooperative medical system,like the insurance,has the adverse selection,this article points out the concept of adverse selection,and raises some efficient way to control the adverse selection.

AIM:Neural stem cells(NSCs)and their supporting factors are related to nerve regeneration after central nerve injury, but there are few evidences for the changes of NSCs and their derived supporting factors.This study explores the change of NSCs and gene expression of stem cell-derived neural stem/progenitor cell supporting factor(SDNSF)in the injured spinal cord tissues of rats,and investigates the relation between the NSCs and expressions of SDNSF. METHODS:The experiment was carried out in the laboratory of Nantong University Neuroscience Institute between September 2005 and March 2006.①A total of 27 SD rats,cleaning grade,were offered by the Animal Experimental Center of Nantong University.Twelve rats used for immunohistochemistry assay were divided into sham operation group and spinal cord injury group,while other 15 rats used for RT-PCR detection were assigned into sham operation group and spinal cord injury group.All the disposals were in agreement with the ethical standard for animals.②The spinal cord contusion model of rats was established according to Allen's falling strike method.Laminectomy was conducted without strike in the sham operation group.The quantity and morphology of NSCs,gila cells and SDNSF-positive cells were observed by immunochemistry to detect expressions of nestin,glial fibrillary acidic protein(GFAP)and SDNSF in the injured spinal cord on days 8 and 16 after injury.The expression of SDNSF mRNA in the injured spinal cord was studied by semi-quantitative RT-PCR on days 4,8,12,16 after injury. RESULTS:①There were nestin-positive cells about central canal of spinal cord in the sham operation group.The nestin-positive cells showed erupted roots,migrated peripherally and proliferation,some GFAP-positive cells emerged and the SDNSF-positive cells were observed,which were similar to neuron on morphology on day 8 after injury.The quantity of nestin-positive cells decreased obviously,there were a number of GFAP-pesitive cells and few SDNSF-positive cells were showed on day 16 after injury.②RT-PCR revealed that SDNSF mRNA expressed in normal rate,and the expression was up-regulated on day 4 after injury,peaked on day 8,and decreased to the normal level on day 16. CONCLUSION:①There are some NSCs about the central canal of spinal cord,which proliferate and soon may differentiate to gila cell after injury in normal rats.②SDNSF is expressed in the normal spinal cord.The expression of SDNSF mRNA in the spinal cord varies with injured time,accompanying the proliferation and differentiation of NSCs. There are intimate relations between NSCs and expression of SDNSF in the repair of spinal cord injury.

Pancreatitis is a common complication of endoscopic retrograde cholangiopancreatography( ERCP). Patient selection and risk factors assessment are critical elements for preventing post-ERCP pancreatitis(PEP). Pancreatic stent plays a role in prevention of PEP,but with limitations. Rectal administration of NSAIDs is an effective way to reduce the incidence of PEP. Effect of other drugs for preventing PEP is unclear. This article reviewed the advances in study on PEP.

Objective Toinvestigatetheriskfactorsforthedevelopmentofpost-endoscopicretrogradecholangiopancreatographypancrea-titisandhyperamylasemia(PEPH)afterendoscopicretrogradecholangiopancreatography(ERCP).Methods Theclinicaldataof233pa-tients who underwent therapeutic ERCP from January 2012 to December 2014 in our hospital and met the inclusion criteria were analyzed ret-rospectively.Basic information including age,sex,drinking history,single stone,triglyceride,and nationality was extracted,and detailed information on intraoperative procedure,intraoperative diagnosis,and complications for ERCP was carefully recorded.Chi-square test was applied for comparison of categorical data between groups,and Logistic regression analysis were applied to determine the risk factors for the developmentofPEPH.Results TheincidenceofPEPHwas26.6%(62/233).Univariateanalysisshowedthatfemalesex(χ2=3.320, P=0.045),drinking history (χ2 =6.108,P=0.010),common bile duct stenosis (χ2 =5.767,P=0.012),single stone (χ2 =9.121, P=0.020),juxta-ampullary duodenal diverticula (χ2 =28.170,P<0.001),and abnormal triglyceride (χ2 =54.717,P<0.001) might be the risk factors for PEPH,and these factors differed significantly between the two groups.Multivariate analysis showed that female sex (OR=1.616,P=0.025)drinking history (OR=2.360,P=0.010),common bile duct stenosis (OR=2.163,P=0.012),juxta-ampullary duodenal diverticula (OR=5.502,P<0.001),and abnormal triglyceride (OR=12.924,P<0.001)were independent risk fac-torsforPEPH.Conclusion Femalesex,drinkinghistory,commonbileductstenosis,juxta-ampullaryduodenaldiverticula,andabnor-mal triglyceride are independent risk factors for PEPH,and targeted preventive measures for such high-risk populations can effectively re-duce the incidence of PEPH.