Objective:To analyze the clinical application of navigation bronchoscopy system, and improve the accuracy during routine use.Methods:Through discussing ENB, System composition, clinical application and environment, the paper presents the clinical application standard and working environment standard of the navigation bronchoscopy system.Results:Electromagnetic navigation bronchoscopy(ENB) combined with the advantages of electromagnetic technology, virtual bronchoscopy and 3D CT reconstruction, which can perform the accurate localization of peripheral pulmonary lesion and mediastinal lymph node to acquire tissue samples for pathological diagnosis. ENB-guided localization can be used in interventional therapy(local injection or radioactive seed implantation).Conclusion:Navigation bronchoscopy system shows the merit of minimally invasive technique and medical visualization. It is a great progress in interventional pulmonology history.

Objective:To have a research and application on management system of cold chain facilities temperature control in the hospital and real-time monitor the temperature and humidity changes of cold-chain facilities.Methods: Based on RFID radio frequency, Internet of things and computer technology to realize the construction of the system hardware and software platform, through commissioning and case analysis for system improvement, the system was popularized and applied comprehensively.Results: After one year’s trial run, it showed that the system could accurately and effectively monitor the abnormal temperature and humidity of the hospital’s cold chain facilities and give the acousto-optic hint and remote alarm in time, effectively improving the management level of cold chain temperature control.Conclusion: The system could guarantee the fine management of the cold chain facilities temperature control, provide safe and traceable historical data, and put forward a new solution for building the quality and safety of cold chain facilities temperature control system.

Objective In order to investigate and evaluate the heating-ventilating-air conditioning (HVAC) systems in the public areas and therefore provide the support for the hygiene management. Methods According to Hygiene Criterion of HVAC System, 10 HVAC systems in 10 star grade hotels in Nanchang were selected and PM10 and the total count of aerobic bacterial in the air condition systems, the collected dust weight and the total count of aerobic bacterial in the dust in the inner surface of ventilation vessels, the legionella bacteria in cooling tower water were determined. Results The HVAC systems in the 10 hotels, 90.1% for the PM10 and 73.3% for the total count of aerobic bacterial in the air condition systems, 8.9% for the collected dust weight and 86.7% for the total count of aerobic bacterial in the dust in the inner surface of ventilation vessels were over the standard limits, the legionella bacteria were detected in two of the investigated HVAC systems cooling tower water. Conclusion The hygienic problems are existed in the ventilated systems in the air conditioners in the pubic places.The health supervision and monitoring must go on enhancing.

Annexin A5 is a member of Ca 2+-dependent phospholipid binding proteins family .It exists in histocytes . And because of its unique structure , Annexin A5 plays an important role in many critical functions such as cell in-side/outside anticoagulation and pro-apoptosis .Moreover , Annexin A5 has certain relevance with many diseases such as systemic lupus erythematosus ,tumour and other diseases .

Objective To investigate the feasibility and clinical effect of hip joint arthrodesis with the application of Kirschner wire and reconstruction plate in the treatment of advanced tuberculosis in total hip joint.Methods A total of 30 patients with advanced tuberculosis of the hip in our hospital from March 2014 to April 2016 were divided as Kirschner wire group and reconstruction plate according to different surgical methods,with 15 cases in each group.The operation time,bleeding volume during operation (intraoperative and postoperative),fusion time,postoperative complications of two groups were observed and compared.Results Patients of two groups reached bony fusion.Compared with reconstruction plate group,Kirschner wire group had less blood loss,shorter operative time,longer fusion time,the differences were statistically significant (P ＜ 0.05).Conclusion Both Kirschner wire fixation and reconstruction plate arthrodesis are effective for patients with advanced tuberculosis of total hip joint,but the clinical efficacy of Kirschner wire fixation in hip arthrodesis was significantly better than the reconstruction plate.

Objective To establish the method for quality standard of Fangzhi Biyan Tablet. Methods Radix Angelicae Dahuricae, Radix Paeoniae Alba, Radix Saposhnikiviae and Radix Glycyrrhizae in the tablet were identified by TLC. The content of Imperatorin in this medicine was determined by HPLC. The Agilent Eclipse XDB-C18 (4.6 mm?250 mm, 5 ?m) was used, mobile phase was methanol-0.1%phosphoric acid solution (65∶35), flow rate was 1.0 mL/min, detection wavelength was 248 nm. Results The TLC spots developed were fairly and simply identical. HPLC was accurate and reproducible. Imperatorin show a good liner relationship at range of 0.076～0.684 ?g, the recovery was 98.63% (RSD=1.52%). Conclusion These methods can be used to control the quality of Fangzhi Biyan Tablet effectively.

OBJECTIVE: To observe the effects of Spleen- strengthening decoction on GLP- 1 in patients with type II diabetes. METHODS: A randomized double- blind placebo- controlled test was conducted, and through observations for 8 wks, the changes of the two patient groups in plasma glucose, HbA1c, plasma GLP- 1 and Glucagon as well as TCM symptom score were measured for comparison. RESULTS: Before administration of Spleen- strengthening decoction, the two groups showed no significant difference in all of the indexes. After administration, the treatment group recorded lower indexes in fasting plasma glucose, HbA1c and plasma Glucagon, but higher index in fasting plasma GLP- 1, with no significant difference seen in other indexes. CONCLUSIONS: Spleen- strengthening decoction can improve glucose metabolism through heightening fasting plasma GLP- 1, and lowering both Glucagon and fasting plasma glucose.

Granular cell tumor of the breast (GCTB) is a rare tumor which stems from Schwann cells.It is a largely benign tumor,but in the literature extremely infrequent cases can exhibit malignant characteristics.It tends to a particular problem as its characteristics are similar with breast carcinoma macroscopically,clinically,and radiologically.Typically,GCTB is benign and solitary lesion,yet including atypical GCTB and malignant GCTB,they can co-localize with breast malignancies multicentricity.The histopathological and immunohistochemical detection is the gold standard for diagnosis of GCTB up to now.And local expanded resection is the main treatment method at present.

Objective To observe the effects of insulin-like growth factor-2 (IGF2) in the course of mouse embryon-ic stem cells induced to differentiate into islet-like cells. Methods Mouse ES cells were induced to differentiate into islet-like cells in vitro. The expression of islet specific markers was tested by RT-PCR and immunofluorescence assay. RT-PCR/RFLP was used to test the imprinted genes IGF2 parental expression in cells at different stages. Results Islet specific mark-ers were expressed in differentiated cells, such as insulin, glucagon and C-peptide. PCR-RFLP showed that imprinted genes IGF2 derived from embryonic stem cells were biallelic expression and loss of imprinting. Conclusion Gene imprinting sta-tus of IGF2 was changed in differentiated cells in vitro.

Purpose To analyze the clinical pathological characteristics and pathological diagnosis and prognosis of spindle cell carci-noma of the breast. Methods Three cases of spindle cell carcinoma of the breast were studied by morphological and immunohisto-chemical EnVision techniques. Results The females were 48, 63 and 71 years old. The tumors located in the right breast with 4. 0 cm × 3. 0 cm × 3. 0 cm, 3. 0 cm × 2. 0 cm × 2. 0 cm and 3. 5 cm × 2. 8 cm × 2. 3 cm in size and showed cystic lesion. The neoplasm was composed of bland spindle cells and mimicking fibromatosis. Immunohistochemical staining showed that spindle cells were positive for CK(AE1/AE3), CK(34βE12), CK14, CK5/6, p63 and vimentin, negative for ER, PR and c-erbB-2. Ki-67 was positive in 20%, 25% and 20% of the cells. Conclusion Spindle cell carcinoma of the breast is a rare subtype of the metaplastic carcinoma which tend to show cystic changes. It is important to make a definite diagnosis which combine histopathologic features and immunophe-notyping.