Objective To explore the hypersensitive c‐reactive protein(hs‐CRP) combined N‐brain natriuretic peptide precursor application value in heart failure .Methods 50 cases of heart failure patients of our hospital as research object ,this group of patients as experimental group .In order to make the research more persuasive ,in addition to select 50 cases of healthy people as control group .Experimental group patients to standards as set by the u .s .New York heart association ,according to the grouping of 24 pa‐tients with rank Ⅱ ,Ⅲ level 15 patients ,11 patients with Ⅳ level .Using colloidal gold method of N‐terminal patients plasma brain natriuretic peptide precursor concentration and left ventricular ejection fraction ,the concentrations of plasma brain natriuretic pep‐tide precursor N‐end needs were determined and the concentration of the hospital for five days prior to admission .Measurement by scattering turbidimetry ,and also need the hs‐CRP serum levels .Results Compare two groups of measuring results were found that the experimental group than the control group of N‐the brain natriuretic peptide precursor level obviously higher ,and the left ven‐tricular ejection fraction was lower than the control group .For the hs‐CRP serum levels of the experimental group is significantly higher than the control group ,cardiac function classification as the increasing of the number of this gap is widening .At levels Ⅱ -Ⅳ obvious the differences between the three ,with statistical significance(P<0 .05) .Conclusion hs‐CRP and N‐brain natriuretic peptide precursor concentration can be as an important indicator of heart failure determination ,has united both in clinical can more effectively to diagnosis heart failure .hs‐CRP in combination with N‐terminal brain natriuretic peptide precursor obvious effects of application in heart failure ,is worth popularizing in clinical use .

Objective To perform the performance evaluation on the automatic biochemical analyzer with the national standards (YY/T0654-2008).Methods The stray light,absorbance accuracy,absorbance stability and repeatability,absorbance linearity, sample carrying contamination rate,sampling accuracy and repeatability,repeatability of TP,ALT,BUN,etc.in the ADVIA 2400 automatic chemical analyzer were detected according to the national standard(YY/T0654-2008).Results The stray light detection value of the ADVIA 2400 analyzer was 6.2Abs;the error of absorbance accuracy was 0.002 9 and 0.007 5 on the absorbance of 0.5Abs and 1.0Abs;the absorbance stability was 0.000 52;the coefficient variation(CV)of absorbance repeatability was 0.12%;the absorbance linearity range was 0.000 2-3.305 7;the sample carrying contamination rate was 0.1% The sampling accuracy and repeatability conformed to the requirement;CV of repeatability of TP,ALT and BUN was 0.6%,1.2% and 1.2% respectively. Conclusion The measurements of various items of the ADVIA 2400 automatic biochemical analyzer meet the national standard (YY/T0654-2008).

Infection of Entamoeba histolytica with chytridiaceous fungus Sphaerlta was observed in some specimens obtained from a farmer and stained with iron-haematoxylin. The fungi were found in 78% of tne cysts, mostly immature ones. Within the amoebae this parasite occurred singly, in groups, or in the form of a sporangium. It was located in the cytoplasm, the glycogen mass or the chromatoidal bars.In the same specimen, the parasitic fungus was also found in 18% of E. coli cysts; in 11% of E. nana cysts; while only one of 16 E. hartmanni cysts was parasitized.It is an interesting case of superimposed parasitism so far reported in China as well as a rare rase of several species of amoebae being heavily involved with the same in the scientific literarure.

Objective To study the changes of plasma brain natriuretic peptide(BNP)level in patients with atrial fibrillation(AF),and explore the relationship between plasma level of BNP and paroxysmal AF,persistent AF,rheumatic AF and non-rheumatic AF.Methods In total,158 outpatients and inpatients diagnosed as heart diseases were selected from the Affiliated Hospital of Inner Mongolia Medical College during October 2005 to January 2007,83 with AF and 75 without AF.Their cardiac function Was evaluated according to the classification system of the New York Heart Association(NYHA)in the United States.Plasma level of BNP was measured with bi-monoclonal antibody microparticulate enzyme immunoassay(MEIA).Results Plasma BNP level was significantly higher in AF group[(112±75)ng/L]than that in non-AF group[(39±26)ng/L,P＜0.05]with same ventricular function.No significant difference in plasma level of BNP between the groups with paroxysmal AF[(113±84)ng/L]and with persistent AF[(110±69)ng/L]was found.Plasma level of BNP Was(84±73)ng/L in the group with rheumatic valvular AF,not significantly different from that in non-valvular AF[(117±76)ng/L,P＞0.05].Conclusion Plasma BNP level is higher in AF group than that in non-AF group,but no significant difference in plasma BNP level between patients with paroxysmal AF,persistent AF,rheumatic valvular AF and non-rheumatic AF,under same ventricular function,was found.

Objective To explore the clinical value of prenatal screening by detecting α-fetoprotein(AFP),β-human chorionic gonadotrophin(β-HCG)and free estriol(u-E3)levels in the second trimester of pregnancy.Methods AFP,β-HCG and u-E3 were de-tected in 3 595 second trimester pregnant women by chemiluminescence.The risk value was calculated by combining with the factors of age,pregnant weeks,body weight,etc.Then the high-risk pregnant women were carried out the amniotic fluid cells chromosome karyotype analysis and B-ultrasonic examination.Results Among 3 595 pregnant women,the screening positive rates of Down′s syndrome(DS),Edward′s syndrome(ES)and neural tube defects(NTD)were 3.70%(133/3 595),0.11%(4/3 595)and 1.44%(52/3 595)respectively.In the prenatal screening,there were 189 cases of high risk pregnant women,among them 87 cases were per-formed the prenatal diagnosis of amniotic fluid cells chromosome karyotype analysis and B-ultrasonic examination,6 cases were defi-nitely diagnosed,in which 3 cases were DS,2 cases were NTD and 1 case was ES.Conclusion Prenatal screening is a non-invasive detection method and has the important clinical application value for avoiding the birth of DS,ES and NTD neonates.

This study was aimed to observe the effect of Xiao-Chai-Hu Decoction (XCHD) with different doses of Bupleurum on body temperature and content of IL-1β, IL-6 and TNF-α in serum among fever rat models induced by lipopolysaccharide (LPS) in order to and investigate its action mechanism. A total of 50 rats were randomly divid-ed into 5 groups, including the normal control group, fever model group, XCHD group (high-dose), XCHD group (middle-dose), and XCHD group (low-dose). The fever rat model was established by intraperitoneal injection of LPS (20 μg/kg). At the same time, all rats were given corresponding XCHD or saline solution by gavage. The body tem-perature of rats (Ti) was measured every 30 min for 240 min after the injection of LPS. Change values of body tem-perature △T(△T=Ti-T0) were calculated. After the last measurement of body temperature, blood was taken for the de-tection of IL-1β, IL-6 and TNF-α level in serum with ELISA. The results showed that the body temperature of XCHD group (high-dose) was obviously decreased at the time point of 3 h, 3.5 h, 4 h (P< 0.01, or P< 0.05). This temperature reducing effect at the time point of 3.5 h and 4 h was better than the XCHD group (middle-dose) and XCHD group (low-dose, P< 0.01, or P< 0.05). Compared with the fever model group, all three XCHD groups can obviously reduce the IL-1β level (P< 0.05). Among them, the XCHD group (high-dose) had obvious effect (P<0.01). Compared with the fever model group, the XCHD group (high-dose) can obviously reduce contents of IL-6 and TNF-α in serum (P< 0.05). It was concluded that XCHD can reduce fever induced by LPS in rats and decrease contents of IL-1β, IL-6 and TNF-α in serum. This effect has a positive dose-effect relationship with the dose of Bu-pleurum in a certain range.

Dipeptidyl peptidase-4 (DPP-4) inhibitors, a new kind of oral hypoglycemic drugs, can significantly lower blood glu-cose levels in the patients with type 2 diabetes without common side effects such as body weight gain, hypoglycemia and gastrointestinal disturbances. Therefore, DPP-4 inhibitors play an increasingly important role in the treatment of type 2 diabetes. In this review, the re-search progress in the action mechanism, pharmacokinetics and clinical applications of DPP-4 inhibitors was summarized in order to provide reference for the clinical application.

Objective To study the skills and safety of breast conserving surgery in radical mastectomy.Methods A total of one hundred and seventy-eight patients with early breast cancer were randomly divided into two groups: 93 cases in the breast conserving surgery group and 85 cases in the radical group,according to the surgical method.The patients in the breast conserving surgery group received stageⅠbreast conserving surgery after modified radical mastectomy for breast cancer,while the patients in the radical group adopted modified radical mastectomy.Then the two groups were compared in terms of postoperative complications,breast appearance evaluation,follow-up on quality of life and tumor recurrence.Results There were not statistically significant differences in postoperative complication rate between the two groups(30.11% vs.23.53%, χ2=0.976,P=0.323);the rate of excellent breast appearance in the conserving surgery group was 86.02%,significantly higher than that of the radical group(38.82%,χ2=42.675,P<0.001);the scores of physiological status,psychologic status,social function and environmental circumstance in the conserving surgery group were significantly higher than those of the radical group,the differences were statistically significant(P<0.05);the difference on tumor recurrence rate of the two groups was not statistically significant(χ2=0.009,P>0.05).Conclusion After radical mastectomy,stageⅠbreast conserving surgery could help patients maintain their breast appearance and improve their life quality,without any increase in the rate of postoperative complications and long-term local morbidity.It is a safe and reliable surgical method.

Objective To compare the long-term outcome between breast-conserving therapy with mastectomy therapy for early stage invasive breast cancer through a Meta analysis of the randomized controlled trials published worldwide.Methods Cochrane systematic evaluation was used to search through Cochrane libraries of clinical comparative trials,PubMed,Embase,Cancer Lit,and so on.The quality of literatures was independently evaluated and cross-checked by two evaluators,indicators for assessment including death number at the end of follow-up,locoregional and total recurrence.The results were analyzed with RevMan 4.2.2 software.Results Six articles were involved in the Meta analysis with total 3 933 patients.No statistical difference was found in the death at the end of follow-up between breast-conserving therapy group and mastectomy therapy group(OR=1.05,95% CI=0.93-1.19,P=0.45).Locoregional and total recurrence rate of breast-conserving therapy group were statistical higher than those in mastectomy therapy group(OR=1.64,95% CI:1.10-2.44,P=0.01;OR=1.42,95% CI:1.22-1.64,P

Adult ; Female ; Humans ; Male ; Middle Aged ; Occupational Exposure ; analysis ; Pneumothorax ; complications ; etiology ; Silicon Dioxide ; analysis ; Silicosis ; complications ; etiology