Objective: To investigate the effects of morphine,tramadol and lornoxicam on the secretion of inflammatory mediators by rats'peripheral blood mononuclear cells(PBMC) at their analgesic concentrations. Methods: Mononuclear cells were isolated from peripheral blood of male SD rats by the Ficoll-Hypaque method,and treated with Morphine(50 ng/ml),Tramadol(500 ng/ml) or Lornoxicam(300(ng/ml)) respectively with or without stimulation by Lipopolysaccharide(LPS) 2 ?g/ml.After incubation for 24 h,concentrations of TNF-?,IL-6 and IL-10 were determined in the cell culture supernatants by ELISA. Results: Levels of TNF-?,IL-6 and IL-10 were reduced significantly in morphine groups with or without LPS;Tramadol reduced levels of these inflammatory mediators significantly in the groups stimulated with LPS,while it had no effects on the cells cultured in normal conditions;Lornoxicam reduced IL-6 and increased IL-10 levels in either groups. Conclusion: Morphine nonselectively suppressed mediator secretions either under inflammatory or physiological conditions;Tramadol reduced inflammatory mediator secretion in LPS stimulating group,thus could benefit patients with potential sepsis;Lornoxicam significantly reduced proinflammatory cytokine IL-6 secretion and increased anti-inflammatory cytokine IL-10 secretion,and may be a choice for postoperative hyperinflammation.

Objective To explore the effect of mon-suturing peritoneum in perforating purulent infection of incisional wound of appendicitis. Methods907 patients suffered purulent perforating and gangrenous appendicitis were divided randomly into two groups:the non-suturing group of 355 patients and the control group of 552 patients.Of the non-suturing group,peritoneum was not sutured when his/her abdominal cavity was closed.It was just on the contrary to the control group.Then examined whether eoncotic rythrogenic or tender incision occurred,or whether purulent secretion flowed outside from the operative incision.These items below were also been recorded carefully:the average number of days when the temperature was abnormal,the grades of healing of incision and the average hospital stay. ResultsIn the non-suturing group,21(5.9%)patients suffered postoperative infection,the number of days of abnormal temperature was(3.8 ± 1)d on average,and the average hospital stay was(7.5 ± 1)d;in the control group,119(21.6%)patients suffered postoperative infection,the number of days of abnormal temperature was(4.3 ± 1)d on average,and the average hospital stay was(12.9 ± 1)d.First rate healing of incision in the non-suturing group was far higher than that in the control group(P＜0.05). ConclusionNon-suturing peritoneum could help internal drainage peritoneum,which was an efficient way to guard against the postoperative infection.And non-suturing was also of great significance to reduce the average number of days of abnormal temperature and hospital stay.Meanwhile,the probability of ankylenteron and incisional hernia was not increased.

Methods A cluster random sampling method was used to select left-behind students in Grade 3 to 6 in 5 primary schools in the southern rural area of Ningxia Hui Autonomous Region. Their general information were collected, their social anxiety and loneliness were evaluated by the Child Social Anxiety Scale ( SASC ) and Children's Loneliness Scale ( CLS ). The multivariate linear regression model was used to analyze the influencing factors for the social anxiety of left-behind children.

BACKGROUND:The essence of cell differentiation is a selectively intra-cellular gene expression,which results in specific proteinic synthesis and causes changes in biochemistry,structure and function.Thus,original proteomics and a single protein analysis can not meet the requirement in study.Proteomics technology provides a powerful tool due to the large scale,systemical study of protein transformation and interaction,which can be used for exploring molecular mechanism of bone mesenchymal stem cells(BMSCs)during directional differentiation.OBJECTIVE:To introduce proteomics,to summarize the research of proteomics in directional differentiation of BMSCs,and to forecast the development of proteomics research methods.METHODS:To search articles highly related with BMSCs,cell differentiation,and proteomics published on CNKI (www.cnki.net/index.htm),Sciencedirect(http://www.Sciencedirect.com),I.S.I(http://www.isiwebofknowledge.com)were searched,and the key achievements were included in the analysis.RESULTS AND CONCLUSION:A total of 29 documents were reviewed,and the experiences in the application of proteomics technology in the directional differentiation of BMSCs were summarized.With the innovation and development in methodology and technology,proteomics will become a powerful tool for us to study the potential mechanisms of BMSCs directional differentiation.

Objective To apply early comprehensive rescue including embolism and microsurgery for trea- ting acute ruptured intracranial aneurysm in order to lower mortality and disability.Methods 120 patients with rup- tured intracranial aneurysm were treated by early comprehensive rescue.An cases were given support treatment,e- mergency rescue and DSA after hospitalization.Embolism and microsurgery were done within 72 hours and releasing bleeding CSF were done after surgery to prevent complications.Results Good recovery was over 80%(excellent in 95 ases,light disability in 18 cases,heavy disability in 4cases,and death in 3 cases).2 cases relapsed during hospi- talization.5 cases were coma induced by serious cerebral vesospasm.Conclusion Early comprehensive treatment including embolism and microsurgery complication prevention may reduce rebleeding,serious vasospasm and hydro- cephalus,1ower mortality and improve prognosis.

Objective To study the skills and safety of breast conserving surgery in radical mastectomy.Methods A total of one hundred and seventy-eight patients with early breast cancer were randomly divided into two groups: 93 cases in the breast conserving surgery group and 85 cases in the radical group,according to the surgical method.The patients in the breast conserving surgery group received stageⅠbreast conserving surgery after modified radical mastectomy for breast cancer,while the patients in the radical group adopted modified radical mastectomy.Then the two groups were compared in terms of postoperative complications,breast appearance evaluation,follow-up on quality of life and tumor recurrence.Results There were not statistically significant differences in postoperative complication rate between the two groups(30.11% vs.23.53%, χ2=0.976,P=0.323);the rate of excellent breast appearance in the conserving surgery group was 86.02%,significantly higher than that of the radical group(38.82%,χ2=42.675,P<0.001);the scores of physiological status,psychologic status,social function and environmental circumstance in the conserving surgery group were significantly higher than those of the radical group,the differences were statistically significant(P<0.05);the difference on tumor recurrence rate of the two groups was not statistically significant(χ2=0.009,P>0.05).Conclusion After radical mastectomy,stageⅠbreast conserving surgery could help patients maintain their breast appearance and improve their life quality,without any increase in the rate of postoperative complications and long-term local morbidity.It is a safe and reliable surgical method.

OBJECTIVE:To test and verify the function of Composite Radix Sophora Flavescentis injection in immunologic enhancement.METHODS:A total of 121 patients with lung cancer were randomly assigned to 2 groups:the treatment group(n=61)received common chemotherapy for 6wk plan in combination with Composite Radix Sophora Flavescentis injection(20mL,iv gtt)qd for the first 10 to 14 days,while the control group(n=60)received common chemotherapy alone for 6wk.The indexes of immunity in both groups were detected before and after treatment.RESULTS:The index of immunity in the treatment group increased significantly as compared with before treatment(P

30 mm Hg) was diagnosed with Doppler echocardiography in 28 patients with SSc.Twenty patients have isolated PHT,while 8 patients were of secondary PHT which was due to severe pulmonary fibrosis.The levels of albumin, ? globulin ,IgA,IgG and CRP in serum of patients with PHT were significantly higher than those without PHT ( P

A spinal cord injury at a level above T1 often results in a partial or complete loss of the hand function. Transcutaneous functional electrical stimulation is a method to stimulate the handicapped limb muscle by low frequency impulsive current to generate muscle contraction. The immediate effect can replace or correct the lost function to promote function reconstruction through adjusting high-level nerve center and improve daily activity. Functional electrical stimulation provides a efficient therapy for the rehabilitation of patients with spinal injury, and brings a hope to improve the quality of life of paraplegina patients. However, transcutaneous functional electrical stimulation requires assistance to place the equipment on the patients and technical support. Both patients and their families often have too high expectations from this therapy, and become disappointed since the results are not satisfactory. Therefore, we should continue to carry out studies on the parameters and equipments of functional electrical stimulation, and renovate the stimulator to make it more convenient and efficient.

Objective:To compare the clinical and imaging characteristics between central nervous system vasculitis(CNSV)and mitochondrial encephalomyopathy(ME),so as to analyze the differential diagnosis of the two disorders.Methods:Clinical data on seven patients with CNSV and five with ME were retrospectively analyzed.The clinical manifestations,laboratory parameters,imaging features and histological characteristics were compared to screen for the evidence of their differential diagnosis.Results:The MRI results of both CNSV patients and ME patients(MELAS type)showed a multi-lesion pattern.The symptoms of CNSV patients included headache,limbs weakness,and erythrocyte sedimentation rate(ESR)increase.The symptoms of MELAS patients included epilepsy and increased serum lactic acid.The electroencephalographic manifestations of both diseases were abnormal:CNSV patients mainly had diffused lesions accompanied with limited alterations;ME patients had evidence of epileptic discharge,which was consistent with the clinical symptoms.Conclusion:Clinical manifestations of CNSV and ME patients are more valuable than imaging findings in the diagnosis of the two diseases.CNSV is characterized by vascular disorders and inflammatory reactions;ME is characterized by abnormal energy metabolism and severe damage of gray matter.The final diagnosis should depend on laboratory and histological examinations.