We studied the changes in canine leukocytic glucocorticoid receptors during endotoxin shock. Blood samples for analysis were collected and leukocytes were isolated Just prior to, 2 and 6 hours after endotoxin administration. Employing ~sH-Dexamethasone (~3H-Dex) as a ligand, we studied ~3H-Dex specific binding of the leukocytes in dogs and their changes during endotoxin shock. Results from two groups (anesthetized and conscious) showed that the specific binding of the leukocytes decreased significantly in both groups 2 hours after endotoxin administration, but they tended to return to normal level by 6 hours. We also studied the changes of GCRS after cndotoxin injection by Scatchard analysis in conscious dogs. The apparent binding capacity (Ro) seems to unchange but the apparent equilibrium dissociation const ant (Kd) increased significantly 6 hours after endotoxin injection, suggesting that the affinity of GCRs decreased. It may be suggested that perturbations in glucocorticoid hormone action at the receptor level might bc involved in the pathogenesis of cndotoxin shock.

Systematic observations were performed on the affinity constant, (Ka), and maximal binding capactiy (MBC) of brain nuclear receptor to T3 and T3 levels in brain at various stages over the whole life span of rats, with 1 day, 2,6,12 and 18 months of age for representing the infancy, childhood, youth, adult and aging stages, respectively. The results might be summarized as the followings: 1) Ka and MBC of nuclear receptor to T3 in cerebra were double of those in cerebel!a, but both did not alter significantly with age and sex; 2) the brain nuclear endogenous T3 levels were high in childhood and gradually decreased thereafter, and elevated slightly in aging; and 3) the occupancy of nuclear receptor by T3 in cerebra and cerebella of rats generally tended to decrease with age but increase slightly when aging.

The two methods of preparing rat liver microsomes were compared. The microsome yield by ultracentrifugation was far grater than that by Ca2+ aggregation(P 0.05) . The results showed that the ig treatment of TMPP at the doses of 50, 100 and 300 mg/kg, respectively, once daily for 10d, had no significant effects on the liver weight, microsome yield, cytoch-rome P-450, Cytochrome b5 and NADPH Cyt C reductase. It was indicated that TMPP metabolized by liver had no ability to induce or inhibit the cytochrome P-450 enzymes in rats. Pretreatment with phenobarbital (75mg/kg?d-1, for 3d ) , a mainly P-450b inducer, significantly decreased the plasma concentrations of TMPP in rats. Therefore, it is suggested that the metabolism of TMPP in rats is probably related to the cytochrome P-450b of liver.

ObjectiveTo investigate the efficacy and safety of intratympanic methylprednisolone perfusion treatment of refractory sudden deafness.MethodsSixty-eight cases of refractory sudden deafness were recruited and divided by random digits table method into intratympanic-treatment group(34 cases)treating with intratympanic methylprednisolone perfusion and control group (34 cases)accepting methylprednisolone intravenous infusion treatment.The curative effect,adverse reaction of two groups were compared and the factors that effected intratympanic perfusion treatment were analyzed.ResultsEight cases of intratympanic-treatment group were cured,twelve cases were markedly effective,nine cases were effective and five cases were ineffective; while seven cases of control group were cured,five were markedly effective,seven were effective and fifteen were ineffective.The total efficiency of intratympanic-treatment group compared with that of control group showed significant difference[ 85.3％(29/34) vs.55.9％ (19/34),P ＜ 0.01 ].Improvement value of impaired frequency examined by pure tone audiometry(PTA) of intratympanic-treatment group was higher than that of control group [ ( 18.0 ± 16.2) dB vs.(8.2 ± 9.8) dB,P ＜ 0.05 ].In intratympanic-treatment group,improvement value of impaired frequency of patients whose age was lower than 50 and disease course was shorter than one month was higher than that of patients whose age was higher than 50 and disease course was longer than one month(P ＜ 0.01 ).The complication rate of intratympanic-treatment group was 8.8％(3/34),and no case had complications such as further decline in hearing.ConclusionThe intratympanic methylprednisolone perfusion treatment is safe and effective for refractory sudden deafness,and deserves further improvement and widely promotion.

ObjectiveTo evaluate the expression of interleukin-6 (IL-6) and interleukin-17 (IL-17) in patients with chronic rhinosinusitis (CRS),and analyze the effects of IL-6 and IL-17 on the occurrence and development of CRS.MethodsSelected 200 CRS patients who planned to receive operation treatment,including 36 cases with type Ⅰ,139 cases with type Ⅱ and 25 cases with type Ⅲ,and they were chosen as CRS group and divided into nasal polyps group ( 109 cases) who had nasal polyps and no nasal polyps group (91 cases) who didn't have nasal polyps.And 50 healthy subjects were chosen as control group.The plasma IL-6 and IL-17 concentration were detected by double-antibody sandwich ELISA method.ResultsThe plasma IL-6 and IL-17 levels of CRS group were (13.23 ±5.94),(9.24 ±4.15) ng/L respectively,significantly higher than those of control group[ (2.26 ± 1.01 ),( 1.58 ± 0.71 ) ng/L] (P ＜ 0.05 ),there was no significant difference between nasal polyps group and no nasal polyps group (P ＞ 0.05 ).The plasma IL-6 levels in type Ⅰ, Ⅱ and Ⅲ were(4.54 ±2.04),(12.90 ±5.80),(27.42 ± 12.32) ng/L respectively,and IL-17 levels were (3.17 ± 1.42),(9.01 ± 4.05),( 19.15 ± 8.61 ) ng/L,there were statistical differences among different clinical types (P ＜ 0.05).The plasma IL-6 and IL-17 level of CRS patients had significantly positive correlation (r =0.519,P ＜ 0.05).ConclusionsThe IL-6 and IL-17 expression are closely relatcd to the occurrence and development of CRS.

Objective To determine the content of L-carnitine injection with HPLC method. Methods Content L-carritine was measured by Diamonsil C18 (200 mm × 4.6 mm,5 μm) column,heptane sodium-phosphate Results L-carnitine in the range of 200-2000 ng had a good linear relationship (r=0.9998),the average recovery rate was 97.88%,RSD 1.07%. Conclusion This method was rapid,accurate,and can be used for determination of Lcarnitine by HPLC.

OBJECTIVE To study the drug resistance,drug-resistant genes and disinfectant-resistant genes of clinical MRSA isolates.METHODS The sensitivity to penicillin and other 15 antibacterial agents was detected in 56 strains of MRSA by K-B paper disk diffusion.The mecA gene of the ?-lactamase and disinfectant-resistant genes qac(A/B)were detected by PCR.RESULTS All of the 56 strains of MRSA were sensitive to vancomycin and teicoplanin.Drug resistant rate of sulfamethoxzole/trimethoprim,nitrofurantoin,rifampin,tetracycline,levofloxacin,clindamycin and azithromycin were 17.9%,23.2%,82.1%,87.5%,89.3%,92.9% and 96.4%,respectively.All of the 56 strains were resistant to erythromycin,gentamicin,amikacin,penicillin,ampicillin/sulbactam,cefoxitin and ampicillin.In all 56 strains of MRSA,54(96.4%)MRSA isolates were mecA positive and 31(55.4%)MRSA isolates were qac(A/B)positive.CONCLUSIONS Clinically isolated MRSAs is multi-drug-resistant and the qac(A/B)positive rate is very high.

Objective:To study the influence of mycophenolic acid(MPA) on survival prolongation of heart allografts with tolerogenic dendritic cells(DCs ) in rat s. Methods:The influence of MPA on CTLA-4Ig-gene transferred donor DCs was observed in vitro morphologically and functionally. The influence of MPA on the survival time of the heart allografts was observed in a Blab/C→C57 Cervical Heterotopic Heart transplantation model with recipients treated by CTLA-4Ig-gene transferred donor DCs. Results:The maturation and aging of DC were delayed and the stimulating ability of DC to recipient splenic cells was impaired significantly with the adding of MPA . Survival time of renal allograft s in CTLA-4Ig-gene transferred DC group was prolonged significantly and further prolonged with the adding of MPA. The allografts functioned well even after 45 d postoperatively in recipient t reated with MPA preconditioned DC. Conclusion:MPA preconditioning impairs the ability of antigen presentation of CTLA-4Ig-gene transferred donor DCs and enhances it s efficacy of survival prolongation of heart allografts in mice.

Objective To study the effect of transurethral vaporization of the prostate (TUVP) and transurethral resection of the prostate(TURP) on benign prostatic hyperplasia(BPH).Methods 218 cases of BPH were treated with TUVP and TURP.Results Followed up for 3 months,the I-PSS and postvoid residual urine de- creased.The quality of life score(QOL) and urine flow rate(Omax) increased significantly after TUVP and TURP. There were no serious complications occurred,and the general results were satisfactory.Conclusion The united us- age of TUVP and TURP is easier to grasp,less invasive to the patients,and the procedure is safe and effective for the treatment of BPH.