Objective To evaluate the effect of laparoscopic operation and laparotomy on immune func -tion in divesting ovarian benign teratoma , and to provide further theoretical evidence for clinical practice .Meth-ods 60 patients with ovarian benign teratoma were selected .30 were conducted by laparoscopic operation ( lapa-roscopic group ) and 30 were conducted by laparotomy ( laparotomy group ) .Operation conditions were compared . White blood cell (WBC) and NEU counts, levels of IL-6,TNF-αand Th17 cells in the 2 groups before surgery, 3 days and 7 days after surgery were detected .Results Surgical time and intraoperative blood loss were signifi-cantly shorter and lower in laparoscopic group than in laparotomy group .Compared to those before surgery , WBC and NEU counts, levels of IL-6, TNF-αand Th17 cells in the two groups increased three days after surgery , and all of these observation targets were much higher in laparotomy group than in laparoscopic group (P＜0.05).There were downtrends of all the observation targets in two groups 7 days after surgery .However , all the observation tar-gets were still significantly higher in laparotomy group than in laparoscopic group ( P＜0.05) .Conclusion For o-varian benign teratoma , laparoscopic operation had less influence on immune function of patients compared with lap -arotomy, which is better for patients'recovery.