Objective To obtain Hangzhou residents' awareness, understanding, demands, and intentions of health examination and explore the factors influencing health examination. Methods Totally 1 183 residents (male: 542, female: 641, aged from15 to 80 years) were investigated by mean of questionnaire which included demographic characteristics, health status, medical behavior and awareness, the subjective reasons of unwilling to take health examination, the intention of choosing an institution, and the data of questionnaire were analyzed using single factor Chi-square test and conditional logistic regression analysis. Results Single factor Chi-square test showed that the factors affecting health examination participation which have statistical significances were as follows, gender (χ2=11.61,P=0.000), age (χ2=9.09, P=0.028), residence registration (χ2=44.16,P=0.000), marital status (χ2=8.96,P=0.03), educational backgroud (χ2=17.33,P=0.000), employment status (χ2=7.97,P=0.005), personal monthly income (χ2=22.82, P=0.000), having any kinds of health insurance (χ2=16.08,P=0.000), and the health examination fees paid by company (χ2=44.78,P=0.000). Conditional logistic regress analysis showed that the related fators which affecting the peoples participating rates of taking health examination are gender (P=0.003, OR=1.782), residence registration (P=0.000, OR=2.208), personal monthly income (P=0.009, OR=1.307), taking any kinds of insurance (P=0.004,OR=2.913)and the company organizing and paying for the healthy examination or not (P=0.000,OR=1.923). Conclusion The participation rates of taking health examination were affected by the factors such as male, younger than 45 years old, divorce, temporary residents, the jobless, low educational diploma and income, not having any insurance, taking the health examinaiton at their own expense and so on.

A novel method was developed for the rapid determination of multi-indicators in corni fructus by means of near infrared (NIR) spectroscopy. Particle swarm optimization (PSO) based least squares support vector machine was investigated to increase the levels of quality control. The calibration models of moisture, extractum, morroniside and loganin were established using the PSO-LS-SVM algorithm. The performance of PSO-LS-SVM models was compared with partial least squares regression (PLSR) and back propagation artificial neural network (BP-ANN). The calibration and validation results of PSO-LS-SVM were superior to both PLS and BP-ANN. For PSO-LS-SVM models, the correlation coefficients (r) of calibrations were all above 0.942. The optimal prediction results were also achieved by PSO-LS-SVM models with the RMSEP (root mean square error of prediction) and RSEP (relative standard errors of prediction) less than 1.176 and 15.5% respectively. The results suggest that PSO-LS-SVM algorithm has a good model performance and high prediction accuracy. NIR has a potential value for rapid determination of multi-indicators in Corni Fructus.

AIM:To study the telomere maintenance mechanism in mesenchymal stem calls(MSCs).METHODS:MSCs were isolated from healthy human bone marrow by their adherence to plastic and then were checked with CD14-FITC,CD45-FITC,CD44-FITC,HLA-DR-FITC,CD34-PE,CD29-PE and CD166-PE.Telomere length and ECTR DNA in MSCs were detected by Southern blotting.The localization of TRF1 and promyelocytic leukemia(PML)in MSCs were detected with immunofluorescence staining.TRAP protocol was performed to detect the telomerase activity in MSCs and MSCs-derived adipocytes.Western blotting and TRAP protocol were applied to measure telomerase activity of MSCs,which were synchronized by serum starvation and aphidicolin treatment.RESULTS:The telomere in length seemed shorter and relatively more homogeneous in MSCs and HeLa cells than that in WI-38-2RA cells.TRF1 did not concide with PML nuclear body in MSCs and HeLa cells while it exclusively did in WI-38-2RA cells.ECTR DNA was negative in MSCs and HeLa cells but positive in WI-38-2RA cells.Telomerase was negative in MSCs but it was positive in MSCs-derived adipocytes detected by TRAP.Moreover,a cell cycle-dependent expression profile of telomerase was found in MSCs when they were synchronized by serum starvation and aphidicolin treatment.Untreated MSCs expressed very low level of telomerase probed by Western blotting with 2C4 mAb,but the telomerase level had significantly increased when these cells were trapped in S phase.CONCLUSION:The telomere of MSCs is maintained by telomerase pathway instead of alternative lengthing of telomere(ALT)and the level of telomerase expression is associated with cell cycle stage.[

Objective To optimize the extraction methods of mitochondrial genome DNA(mtDNA)of Oncomelania hupen-sis. Methods The pyrolysis,protein K variable-temperature digestion and high-concentration potassium acetate purification were applied to optimize the high-concentration-salt precipitation method,and then the optimized method was compared with two common extraction methods,the sucrose density gradient centrifugation method and traditional high-concentration-salt pre-cipitation method. The mtDNA samples were identified by using spectrophotometry,agarose gel electrophoresis and the amplifi-cation products of COX1. The nuclear DNA contamination was tested by the amplification products of ITS. Results The concen-tration and yield of the improved method was significantly higher than those of the traditional method(F=3032.65,10185.00, both P<0.01). The mtDNA samples extracted were essentially free of nuclear DNA and protein,meeting PCR,sequence analy-sis and other molecular biology research requirements. Conclusions The improved high-concentration-salt precipitation meth-od for isolating mtDNA is simple,and it has high yield and low cost. The extracted mtDNA can meet relevant analysis require-ments.