Objective To observe the tissue displacements between the acupoints and non-acupoints using tissue Doppler imaging.Methods Acupuncture bilaterally were performed at Zusanli,Qvchi and corresponding control points 1 cm lateral to them in 30 healthy human subjects.Monitored by the dynamic system for detecting the force of acupuncture needle during the acupuncture process,tissue Doppler imaging was recorded on the condition of keeping the depth of needle insertion and the rotational torque at the same level approximately.Tissue Doppler imaging was also recorded before and after the acupuncture.Results The mean tissue displacements of the aforementioned 2 acupoints were all significantly greater than those of the corresponding non acupoints(P＜0.05).The mean tissue displacements after the acupuncture were all significantly greater than those before the acupuncture(P＜0.001).Conclusions Tissue Doppler imaging provides a new thought and method for the research of the acupoints.

Salmonella enterica serovar Cerro 87, which was isolated from a commercial egg-producing farm, has a phosphorothioated DNA backbone resulting DNA degradation(Dnd) during the pulsed field gel electrophoresis (PFGE) process. In this research, a gene deletion mutant XTG103 was engineered with the entire dnd gene cluster knocked out by double crossover using vector pKOV-kan, and lost Dnd phenotype corre- spondingly. We regulated the DNA phosphorothioation by heterogenous expression of dnd gene cluster with an isopropyl ?-D-1-thiogalactopyranoside (IPTG) inducible promoter PlacZ.

Adult ; Case-Control Studies ; Comet Assay ; DNA Mutational Analysis ; Female ; Humans ; Male ; Micronucleus Tests ; Middle Aged ; Occupational Exposure ; Vincristine

Objective To evaluate the image quality of dynamic three-dimensional contrast enhanced ultrasonography(3D-CEUS) in liver tumors.Methods Twenty-five patients were examed with 2D-CEUS and dynamic 3D-CEUS.Contrast-enhanced computed tomography or magnetic resonance imaging(CECT/MRI) were performed early or late.Two readers reviewed the images of both modalities and graded the images according to diagnostic criteria as follows:tumor blood fusion in arterial phase(unclear-score 0,moderate-score 1,clear-score 2),tumor boundry and relationship with periphery tissues (unclear score 0,moderate-score 1,clear-score 2).The concordance between the two modalities for showing liver tumors was evaluated by Kappa test.Results Twenty-three of 25 danymic 3D-CEUS were displayed successfully versus total objects in CECT/ MRI.Among them,for showing blood fusion,16 lesions scored 2,7 lesions scored 1,2 lesions scored 0 in danymic 3D-CEUS,meanwhile 18 lesions scored 2,7 lesions scored 1,0 lesion scored 0 in CECT/MRI,the Kappa value was 0.65.For showing tumor boundry and relationship with peripherytissues,16 lesions scored 2,7 lesions scored 1,2 lesions scored 0 in danymic 3D-CEUS,meanwhile 21 lesions scored 2,4 lesions scored 1,0 lesion scored 0 in CECT/MRI,the Kappa value was 0.52.Conclusions Danymic 3D-CEUS was shown to be useful for displaying tumor blood fusion,boundry and relationship with periphery tissues in single liver tuomor,the concordance between dynamic CEUS and CECT/MRI for single liver tuomor was fair.For huge liver tumor and multi-tumors,3D-CEUS can not give overall information.

Objective To explore the affecting factors of nursing interns' personal knowledge management (KM) ability and analyze the correlation between their KM ability and clinical practice ability.Methods A total of 345 nursing interns' KM ability and clinical practice ability were investigated by using two scales.Results Affecting factors of nursing interns' KM ability included being a cadre and being interested in nursing specialty or not.Factors affecting nursing interns' clinical practice ability were practice period,academic records,being a cadre,intention to have a nursing job,interest to be a nurse.It also showed significant positive correlation between their KM ability and clinical practice ability (r=0.761); KM ability could explain 59％ of variability of clinical practice ability(R2=0.599).Among the dimensions of KM ability,the highest positive correlation was knowledge management cognition (r=0.741).Conclusions To enhance nursing interns' clinical practice ability,we need cultivate nursing interns' interest to nursing specialty,ability to be a cadre and improve their knowledge management cognition ability.

AIM:To establish the quality standard of Compound Shouwu Granule (Radix Polygoni multiflori,Radix et Rhizoma Thalictri Baicalensis,Herba Rabdosiae Rubescentis,etc.) METHODS:TLC was performed to identify Radix Polygoni Multiflori,Radix et Rhizoma Thalictri Baicalensis,Herba Rabdosiae Rubescentis.HPLC was used to determine 2,3,5,4'-tetrahydroxystilbene-2-0-?-D-glucoside. RESULTS:The study on the quality control showed that the characteristic of identification by TLC was distinct and highly specific.The quantitative evaluation had the linear range of 0.04-0.72 ?g.The average recovery was 99.99% and RSD was 1.3%. CONCLUSION: The method for identification and quantitation was simple,accurate,realizable and reproducible.It can be used effectively for the quality control of Compound Shouwu Granule.

Objective To compare the anti-inflammatory effects between methotrexate(MTX) and Yangqixue Qufengshi Recipe(YQXQFSR) on mice arthritis induced by collagen II.Methods The model of rheumatoid arthritis was induced by using collagen II in C_(57)BL/6?DBA/1 mice.The rats with arthritis were treated with MTX or YQXQFSR respectively.The indexes such as the onset day of collagen induced arthritis(CIA),the level of CII-reactive antibodies and the pathological scores of CIA were assessed.Results The onset day of CIA and the level of CII-reactive antibodies were not influenced in the treatment of mice arthritis with MTX and YQXQFSR respectively.But MTX could alleviate the damage of cartilage [(1.60?1.51) vs(3.56?1.33) scores,P

Objective To analyze the present situation and reasons of short-term unplanned extubation after peripherally inserted central venous catheters (PICC)surgery in cancer patients. Methods A total of 2 458 cancer patients underwent PICC surgery were collected,and patients experienced short-term unplanned extubation were screened out to analyze the general information, catheter situation and reasons of unplanned extubation.Results Totally 2 431 cases (98.90%, 2431 /2458)were successfully followed-up and 103 patients (4.24%,103 /2431)experienced short-term unplanned extubation.Among them,the proportion of patients received primary PICC,pa-tients over 65 years old and patients with catheter located in median cubital vein were 96.12%(99 /103),59.22% (61 /103)and 46.60% (48 /103)respectively.The incidence of unplanned extubation were related to age,vascular choose and whether the primary PICC.Subjective will (61.17%,63 /103)of patients was the main reason for short-term unplanned extubation and the objective reasons were catheter prolapse (10.67%,11 /103),local thrombosis (7.77%,8 /103) and puncture site exudation /phlebitis(6.80%,7 /103),catheter-related infections (5.83%,6 /103)and so on.Conclusion Effective maintenance during the entire process after PICC such as adequate doctor-patient communication,positive patient education,strengthening outside hospital PICC maintenance training as well as establishment of good follow up system can reduce the inci-dence rate of short-term unplanned extubation.

Objective To analyze the present situation and reasons of short-term unplanned extubation after peripherally inserted central venous catheters (PICC)surgery in cancer patients. Methods A total of 2 458 cancer patients underwent PICC surgery were collected,and patients experienced short-term unplanned extubation were screened out to analyze the general information, catheter situation and reasons of unplanned extubation.Results Totally 2 431 cases (98.90%, 2431 /2458)were successfully followed-up and 103 patients (4.24%,103 /2431)experienced short-term unplanned extubation.Among them,the proportion of patients received primary PICC,pa-tients over 65 years old and patients with catheter located in median cubital vein were 96.12%(99 /103),59.22% (61 /103)and 46.60% (48 /103)respectively.The incidence of unplanned extubation were related to age,vascular choose and whether the primary PICC.Subjective will (61.17%,63 /103)of patients was the main reason for short-term unplanned extubation and the objective reasons were catheter prolapse (10.67%,11 /103),local thrombosis (7.77%,8 /103) and puncture site exudation /phlebitis(6.80%,7 /103),catheter-related infections (5.83%,6 /103)and so on.Conclusion Effective maintenance during the entire process after PICC such as adequate doctor-patient communication,positive patient education,strengthening outside hospital PICC maintenance training as well as establishment of good follow up system can reduce the inci-dence rate of short-term unplanned extubation.

Objective To explore the value of plasma C-peptide levels in early prediction of type 2 diabetes mellitus with peripheral sensory neuropathy.Methods The vibration perception threshold,pain,temperature sensation,touch-pressure sensation,ankle reflex was detected in 500 eases of type 2 diabetes mellitus,and the patients were divided into 4 groups according to peripheral sensory nerve test results:normal group (159 cases),mildly abnormal group (120 cases),moderately abnormal group (121 cases) and severely abnormal group (100 cases).Fasting and 2-hour postprandial C-peptide levels were determined and analysed with peripheral sensory nerve changes.The receiver-operating characteristic (ROC) curve was used to find the best critical point for diagnosis of diabetic peripheral sensory neuropathy.Results The fasting C-peptide among 4 groups had no significant difference (F =1.632,P ＞0.05).Two-hour postprandial C-peptide from normal group to mildly abnormal group and then moderately abnormal group gradually increased [(1.110 ± 0.526),(1.324 ± 0.490),(1.573 ± 0.716) μ g/L],while 2-hour postprandial C-peptide in severely abnormal group was significantly decreased and lower than that in normal group,and there were significant differences (P＜ 0.05).The max Youden Index was 0.366 when 2-hour postprandial C-peptide was 1.173 μ g/L.Conclusions The fasting C-peptide might be not related to early diabetic peripheral sensory neuropathy,but 2-hour postprandial C-peptide might be closely related to early diabetic peripheral sensory neuropathy.It is helpful to detect the early diabetic peripheral sensory neuropathy if we can take a dynamical observation of 2-hour postprandial C-peptide.