Objective To investigate the best experimental scheme of Mycoplasma solid culture combined with liquid culture.Methods A total of 961 samples of urogenital tract excretion were collected from March 2016 to June 2016.Both solid culture and liquid culture were performed for detection of Mycoplasma,false positive broths were picked out after 48 h,20 μl of each one was transformed to solid media for subculture,final results were read after 48 h.The experimental data was analyzed to find an optimal combination culture scheme.Results The positive rate of solid culture was 38.7％ (372/961) for UU and 7.8％ (75/961) for MH.The positive rate of liquid culture was 45.27％ (435/961) for UU and 7.08％ (68/961) for MH.The different rate between both methods was 9.47％ (91/961) for UU (x2 =43.61,P＜0.005),and 3.02％ (29/ 961) for MH (x2 =1.24,P＞ 0.05).The different rate was 53.5 ％ (46/86) between primary solid culture and subculture.The positive rate of total (primary solid culture and subculture) solid culture was 41.9％ (403/961) for UU and 8.6％ (83/961) for M H,which produced higher sensitivity than single (primary solid culture or subculture) solid culture.The different rate between the total solid culture and the liquid method was 6.24 ％ (60/961) for UU (x2 =17.067,P＜0.05),and 3.43 ％ (33/961) for MH (x2=5.94,P＜0.05).Conclusion Perform both solid culture and liquid culture for Mycoplasma,pick out those false positive broths for subculture.Total solid culture could be more reliable as final result,for which could combine specificity of solid culture with sensitivity of liquid culture.

Objective To investigate the application value of 16S rDNA-based detection technique in the rapid screening for and confirmation of gonococcemia. Methods A 41-year-old male patient was hospitalized for recurrent regular fever and chills for 1 month. Several days before the admission, he developed urgent micturition, frequent micturition and pain in urination, anemia with emaciation appearance, slightly pale-looking skin and mucous membranes. No petechia, skin eruption or superficial lymphadenectasis was observed, but routine blood test and urine test results were abnormal. No abnormality was found in stool test or hepatic and renal function. DNA was extracted from the blood of the patient and subjected to PCR for the amplification of 16S rDNA followed by sequence analysis and homology analysis. At the same time, bacterial culture of blood and drug sensitivity test for the bacterial isolate were performed. Results Homology analysis indicated that the amplicon sequence was consistent with the known sequence of 16S rDNA of Neisseria gonorrhoeae in GeneBank, which agreed well with the culture result of peripheral blood. Conclusion The detection of 16s rDNA with PCR from peripheral blood is highly efficient, specific, sensitive, rapid and accurate for the screening for and confirmation of gonococcemia.

Objective To investigate the drug-resistance of Staphylococcus aureus induced by erythromycin in vitro and its changes in growth and susceptibility of antibiotics.Methods Erythromycin in vitro induction was conducted with the S.au-reus reference strain ATCC25923 on the serial of erythromycin agar plates.The growth of S.aureus,and the susceptibility a-gainst other antibiotics was compared after induced to the parent strain.Results Resistance to erythromycin was successful-ly,and themaximum MIC was over 256 mg/L.The Erythromycin-resistant S.aureus grew much slower than the susceptible parent,and the strains didn’t have cross-resistance to other antibiotics.Conclusion S.aureus could be induced resistance in vitro by erythromycin,and this resistance inherited stably.Some phenotype and biochemical characteristic features of the strain were changed after induced.

Objective:To investigate the application value of reactive oxygen species (ROS) and adiponectin (ADPN) in the judgment of liver inflammation in chronic hepatitis B virus infection combined with nonalcoholic fatty liver disease (NAFLD).Methods:A total of 159 cases with NAFLD (21 cases), chronic hepatitis B virus infection (57 cases), and chronic hepatitis B virus infection combined with NAFLD (81 cases) were collected between June 2016 to December 2018, and the visited patients diagnosis were confirmed by histopathological examination of the liver. ROS and ADPN level retained in serum was determined by enzyme-linked immunosorbent assay. Histopathological examination of liver tissue was used as the gold standard to discuss the diagnostic value of the serum in patients with chronic hepatitis B virus infection combined with NAFLD for the occurrence of nonalcoholic steatohepatitis. One-way analysis of variance was used for the comparison among multiple groups, and LSD-t test was used for pairwise comparison between groups. Measurement data for non-normal distributions were expressed as M (P25, P75). Comparisons between groups were performed using the Mann-Whitney U or Kruskal-Wallis H test. Chi-square test was used to compare the count data between groups. Correlation analysis was performed using Spearman correlation analysis. Histopathological grouping of liver tissue was used as the gold standard, and the area under the receiver operating characteristic curve was used to evaluate the diagnostic efficacy of the regression formula.Results:(1) In patients with chronic hepatitis B virus infection combined with NAFLD, the levels of ROS in the non-hepatic steatosis group and the mild hepatic steatosis group were significantly lower than those in the moderate and severe hepatic steatosis group, while the ADPN level in the non-hepatic steatosis group was significantly higher than liver steatosis group, P < 0.05. (2) The results of correlation analysis showed that ROS was significantly correlated with NAS score, change in the degree of fatty liver and lobular inflammation (all P < 0.05).There was a significant negative correlation between ADPN and the change in the degree of fatty liver ( P < 0.05). (3) Logistic regression analysis results showed that the diagnostic formula for chronic hepatitis B virus infection combined with nonalcoholic steatohepatitis was 0.02 × controlled attenuation index + 0.584 × white blood cells/10 9 + 0.587 × ROS-10.982. The area under receiver operating characteristic curve of the subject was = 0.896. The sensitivity, specificity, positive and negative predictive value were 97.1%, 71.2%, 64.2%, and 97.9%. Conclusion:ADPN and ROS have certain reference value in differentiating the change in the degree of fatty liver and inflammation in chronic hepatitis B virus infection combined with NAFLD and the diagnostic formula has higher application value in the diagnosis and exclusion of chronic hepatitis B virus infection combined with nonalcoholic steatohepatitis.