目的探讨保留胃管对重型颅脑损伤气管切开患者气管套管拔管后切口闭合及肺部感染的影响。方法64例重症颅脑损伤气管切开患者,按是否保留原有胃管分成实验组(保留原有胃管)和对照组(不保留原有胃管),对两组患者气管套管拔出后切口闭合时间、肺部再次感染等情况进行观察。结果实验组切口闭合时间(2.26±1.94)d较对照组(3.83±3.82)d缩短(P<0.05);实验组肺部再次感染率(14.70%)少于对照组(40%,P<0.05)。结论重型颅脑损伤气管切开患者选择在拔气管套管时保留原有胃管可使切口早期愈合,减少肺部再次感染。

Objective To investigate the effect of acceptance and commitment therapy intervention on post-traumatic growth of patients with concomitant brain injury in group context. Methods From January 2016 to January 2017, 40 patients with recovery from craniocerebral injury such as car accidents and work-related injuries were set as the control group, and regular health education and guidance were conducted twice a week for 6 times. From February to February 2018, 42 patients with recovery from craniocerebral injury such as car accidents and work-related injuries were set as observation groups. The observation group was given a 3-week group acceptance and commitment therapy intervention on the basis of the control group. Before and after the intervention, the simplified Chinese version of the post-traumatic growth rating scale was used for evaluation. Results After intervention, the total scores of life perception, personal strength, new possibility, relationship with others, self-transformation and post-traumatic growth were (18.68 ± 3.86), (11.35 ± 2.67), (10.49 ± 2.48), (14.35 ± 2.95), (10.49 ± 2.32), (65.36 ± 14.28) points in the observation group, and were (13.23±3.10), (9.46±2.41), (6.82±1.72), (11.81±2.06), (7.91 ± 1.89), (49.23 ± 11.18) points the control group, the difference between the two groups were statistically significant (t=7.325-8.289, both P<0.05). Conclusions Group acceptance and commitment therapy intervention can improve the post-traumatic growth level of patients with recovery period of craniocerebral injury, and provide a reliable basis for clinical medical staff to promote positive psychological adjustment of patients with recovery period of craniocerebral injury.

Objective To investigate the effect of SP600125 on the proliferation, cell cycle, apoptosis and invasion of human cervical cancer HeLa cells. Methods CCK-8 method was used to detect the proliferation of HeLa cells treated with different concentrations of SP600125 at different time points. The 20 μmol/L of SP600125 was determined for subsequent experiments. Cell proliferation ability was detected using plate clone formation assay; nuclear morphology was observed by DAPI staining; cell cycle and apoptosis were measured by flow cytometry; cell migration and invasion were detected by cell scratch and Transwell methods; the mRNA and protein levels of p53, Mad2L1 and CDC20 were measured by qRT-PCR and Western blot after SP600125 treatment at different time points. Results Compared with control group (0.1%DMSO), cells proliferative activity were reduced by 10, 20, 30, 40 and 50 μmol/L SP600125 treatment for 24h. Compared with control group, the rate of apoptosis was significantly increased in SP600125 treatment groups, and the cell proportion in G₂/M phase increased (P < 0.001), while the cell proportion in G₀/G₁ phases cells was reduced after SP600125 treatment for 24h and 48h (P < 0.001), and the clonal number, migration and invasion ability of HeLa cells also decreased significantly (P < 0.001). qRT-PCR and Western blot results showed a significant decrease in Mad2L1 mRNA and protein expression (P < 0.05) and a significant increase in p53 and CDC20 mRNA and protein expression (P < 0.01). Conclusion SP600125 can induce cell cycle arrest of cervical cancer HeLa cells in G₂/M phase by upregulating p53 and CDC20 and downregulating Mad2L1 expression, and promote cell apoptosis to inhibit cell proliferation, migration and invasion.