Objective To prove the existence of dry eye in children and to learn the clinical characteristics of them. Design Prospective observational case series. Participants 38 cases(76 eyes) of suspected children of dry eye diagnosed by adult standard and 38 normal children subjects. Methods 38 suspected child patients were studied who were diagnosed clinically with dry eye by adult standard, and were followed up 6 months. The control group consisted of 38 normal children with no significant difference in age. Dry eye examinations including Schirmer test, break-up time(BUT) and fluorescein staining were performed on these two groups. Following items were recorded in 38 suspected children of dry eye, including symptoms and causations. Main Outcome Measures Symptoms, Schirmer test, BUT and fluorescein staining. Results In the 38 suspected patients, frequent blinking was the most common symptom in 21 cases(55.26%), followed by dryness (15 cases, 39.47%), redness (14 cases, 36.84%) and photosensitivity (14 cases, 36.84%). The Schirmer test and BUT were decreased remarkably in the suspected patients compared to those of normal subjects (P=0.0000). In both groups, right eyes were correlated with left eyes in both Schirmer test and BUT. Between these two groups, Schirmer test was nol correlated with BUT. In the suspected patients, the results of BUT and fluorescein staining were improved (P

Objective To approach the influence of erythropoietin on proliferation and differentiation of neural stem cells originated from the hippocampi of adult rats in vitro. Methods Neural stem cells were isolated from the hippocampi of adult rats and cultured in vitro with serum-free incubation and single-cell cloning technique; immunochemistry technique was employed to identify the neural stem cells and neural stem cells-derived progeny. Erythropoietin (EPO) were added to cultures in different concentrations during proliferation and differentiation of neural stem cells, respectively. Results Compared with the control group, EPO supplementation group displayed an average twofold to fourfold increase in Nestin positive cells over a wide range of plating densities during proliferation, whereas showed a striking decrease in Nestin positive cells during differentiation,following an early appearance of Tuj1 or GFAP positive cells. The percentage of Tuj1 positive cells increased significantly to 57% in EPO supplementation group versus 47% in control group (n=12, P

AIM: To establish a HSP90 highly expressing cell line and study the effect of high level of HSP90 on cell stress response. METHODS: The recombined plasimid pSmycHSP, which contains the full length DNA coding for human HSP90?, was introduced into mouse fibroblast cell line NIH-3T3 by electroporation after being subcloned, purified and identified by limited enzyme digestion. Screened by G418, the positive clones were selected and identified by immunofluorescence, immunocytochemistry and Western-blotting. NIH-3T3 cells transfected with empty plasmid served as control, hyperthermia(44 ℃, 20 min, 40 min)was used to simulate oxidative stress. The activity of lactate dehydrogenase (LDH) in supernatant and damage of DNA were detected by automatic biochemistry analyzer and flow cytometer separately to analyze the effect of high-level HSP90 on cell membrane and DNA injuries under stress condition. RESULTS: The rising level of HSP90 was shown by immunofluorescence, immunocytochemistry and Western-blotting in HSP90 overexpressing cell line. There was no difference in the leakage of LDH between HSP90 overexpressing cell line and control, but the damage of DNA was more severe at 44 ℃for 20 min in HSP90 overexpressing cell line than control. Compared with control, the above indices were relieved at 44 ℃ for 40 min in HSP90 overexpressing cell line. CONCLUSION: The NIH-3T3 derived cell line, which stably expressed high level of HSP90, was established. The effect of high-level HSP90 on cells is complex at different intensity of stress, and the protection may be shown at more severe stress circumstance.

AIM: To observe the effect of high temperature on the delayed rectifier K+ channel(I K) in hypothalamus neurons METHODS: The kinetic properties of I K were measured by cell-attached mode of patche-clamp technique RESULTS: The conductance, long-term open constant(? o2 ) and open probability(Po) of I K increased with increase of temperature ( P

AIM: To investigate the effect of Ba 2+ concentration on L-type of Ca 2+ channel in hypothalamic neurons.METHODS: The cell acute isolation technique and cell-attached patch-clamp technique were used. RESULTS : The slope conductance of L-type Ca 2+ channel were 28.6 pS (110 mmol/L) and 19.1 pS (10 mmol/L), and the open probability (NP 0) obviously different with different Ba 2+ concentration as carrier. CONCLUSION: Ba 2+ concentration had the obvious effect on the L-type Ca 2+ channel.

Adaptation, Physiological ; Animals ; Female ; Gene Expression Regulation ; Hot Temperature ; Hypothalamus ; metabolism ; Male ; Rats ; Rats, Sprague-Dawley ; Transcriptome

Objective To make the contrast analysis of concentrated training on clinical skill to college students before probation. Methods The students were devided into traning group (320pers) and contrast group (240pers), the training group undertook concentrated training on clinical skills before probation, while contrast group didn't. To make the contrast analyses on these two groups on medical record writing and to make questionnaire by those clinical teachers. Results The performance of traning group on medical record writing is higher than the contrast group (P<0.01) , and there also were significant difference in physical examination, medical record writing and comprehend capability. Conclusion The performance of clinical skills were elevated by concentrated training,and there were good teaching effects on the clinical teaching by the oncentrated clinical training.

Because of the aggressive threaten of heat stroke and a lack of understanding of the mechanism of action, mammal animal models for experimental heat stroke were well developed. During the past 5 decades, anesthetized mouse, rat, rabbit, dog, baboon and monkey were used as animal model for experimental heat stroke. However, anesthetized mammals models have some limitations, such as neuroprotective effect of anesthetic agents, possible disturbance on injury and recovery of stroke animals by anesthetic agents, difficulty of discussing animal behavior before and after heat stroke, it was also difficult for the models to evaluate cognitive function of animal under hot environment. Considering humanitarian, only awaked and unrestrained mouse heat stroke model was accepted so far. Therefore, we also developed an awaked and unrestrained rat heat stroke model, and found it was helpful to evaluate drug effectiveness for animal behavior and cognitive function under hot environment.
Animals ; Cognition ; Disease Models, Animal ; Heat Stroke ; physiopathology ; Mice ; Rats

Animals ; Blood Pressure ; Heat-Shock Proteins ; metabolism ; Heat-Shock Response ; Hypotension ; metabolism ; Male ; Myocardium ; metabolism ; Nitric Oxide ; metabolism ; Rats ; Rats, Sprague-Dawley

Objective: To establish a new hepatocellular carcinoma cell line EHBC-512 and to analyze its biological property, so as to lay a foundation for future study. Methods: The specimen of liver cancer was resected during operation and was primary cultured. The morphology of cells was observed under optical and electron microscope. The cell cycle was examined with flow cytometry; the chromosome karyotype was also analyzed. The expression of AFP in the culture supernatant was detected by immunofluorescence and chemoluminescence. The cultured cells were implanted into nude mice for observation of tumor formation. Results: The cultured cell line had the character of liver cancer under light microscope, and had rich mitochondria, with obvious nuclear heteromorphism and a chromosome number of 110-120. The doubling time of the cells was 48 h, and the adherence rate was 90%. Immunofluorescence showed that the cells were positive of AFP and CK18 and negative of CEA and CK19. The production of AFP was higher and the content of AFP was higher than 1210 μg/L. The histology of the exografts was similar to that of the primary tumor. Conclusion: We have successfully established a new hepatocellular cell line EHBC-512, which can stably secrete AFP in vitro, providing a novel tool for liver cancer research.