Objective To optimize the culture conditions of four vaccine candidates of severe acute respiratory symptom coronavirus 2(SARS-CoV-2) Omicron variants BA.1,BA.1.1,BA.2 and BA.5 in Vero cells.Methods The harvest time(24,48,72 and 96 h) and MOI(0.01,0.001,0.0001 and 0.000 01) of four Omicron variants cultured in Vero cells were optimized by using cytopathic effect(CPE),viral nucleic acid copy number and viral titer as evaluation indexes.Results The optimum harvest time of the four Omicron variants BA.1,BA.1.1,BA.2 and BA.5 in Vero cells was 72 h,and the optimum MOI was 0.001～0.000 01,0.001～0.000 01,0.01～0.000 01 and 0.01～0.000 01,respectively.Conclusion The culture conditions of four Omicron variants in Vero cells were optimized,which laid a foundation of the development of SARS-CoV-2 Omicron variant inactivated vaccine based on Vero cells.

Objective To investigate the effect of empowerment education on self-management in patients with diabetes mellitus. Methods A total of 128 patients with diabetes mellitus who met the criteria of WHO in 1999 were selected and divided into the control group(62 cases)and the observation group(66 cases). The control group accepted the traditional nursing methods, and the observation group accepted the empowerment education model. FBG, PBG, HbA1c and Diabetes Management Self-efficacy Scale (C-DMSES) were compared between two groups of patients after 3 months and 6 months. Results After the intervention with the empowerment education model, the patient′s FBG, PBG, HBA1c of the control group were (7.69±4.21) mmol/L, (11.78±3.21) mmol/L, (7.95±1.46)%three months after discharge, and (7.21 ± 3.19) mmol/L, (10.48 ± 4.65) mmol/L, (9.15 ± 2.36)% six months after discharge; while in the observation group, FBG, PBG, HBA1c were (6.23 ± 3.59) mmol/L,(9.32 ± 4.06) mmol/L, (6.69 ± 1.10)%three months after discharge, and (6.15 ± 1.89) mmol/L,(7.33 ± 2.10) mmol/L,(6.02 ± 2.17)%. The differences between two groups were significant (t=2.012-5.196, P<0.05). C-DMSES scores of the control group were 103.48 ± 18.41, 134.38 ± 13.01 on admission and six months after discharge, while in the observation they were 102.83 ± 10.91,157.64 ± 19.56. The differences between two groups were significant (t=3.151, P<0.05). Conclusions Empowerment education model can improve the ability of self-management of diabetes patients, improve self-efficacy to improve their own ability to control blood glucose.

Bronchopulmonary dysplasia is one of the most common chronic respiratory diseases in premature infants, especially in very low birth weight infants.Lung immaturity, inflammatory injury, oxidative stress and abnormal repair after injury are the important factors.Leukotriene is an inflammatory mediator of 5-lipoxygenase pathway and participates in the occurrence of bronchopulmonary dysplasia.Montelukast, as a leukotriene receptor antagonist, may play a role in the treatment of bronchopulmonary dysplasia through anti-inflammation, anti-oxidation and anti-fibrosis.This article will review the potential mechanism and related clinical researches of montelukast which is used in treating bronchopulmonary dysplasia.

OBJECTIVE:To observe the cerebral protective effects of dexmedetomidine in brain aneurysm surgery. METH-ODS:48 patients with intracranial aneurysm were randomly divided into observation group and control group,with 24 cases in each group. Observation group was given dexmedetomidine 1 μg/kg,pump time 10 min,at rate of 0.5 μg/kg;control group re-ceived constant volume of normal saline. Hemodynamic changes,the levels of S100β protein and neuron specific enolase (NSE) were compared between 2 groups. RESULTS:The heart rate and mean arterial blood pressure were more stable in the observation group after medication,with statistical significance(P<0.05). The serum level of S100β protein at the end of operation(T5),6 h after operation(T6),12 h after operation(T7),24 h after operation(T8)were(1.52±0.35)μg/L,(1.69±0.33)μg/L,(1.72±0.42)μg/L,(2.08±0.57)μg/L;the serum level of NSE were(10.9±1.2)ng/L,(13.5±1.5)ng/L,(16.8±2.8)ng/L and(19.7±4.3)ng/L in observation group;those were all lower than in control group,with statistical significance(P<0.05). CONCLUSIONS:Dexme-detomidine for cerebral aneurysm operation is helpful for hemodynamics stability,and result in lower S100β protein and NSE. So it is important for cerebral protection.

Objective To observe the effects of dexmedetomidine on serum TNF-αand IL-6 in neurosurgical patients during perioperative period. Methods 60 cases patients with intracranial aneurysm treated in our hospital in January 2014 to August 2014 were selected as the research objects, and they were randomly divided into two groups with 30 cases in each group.Before induction, the observation group adopted 1μg/kg dexmedetomidine with 10 min infusion, then adopted 0.4μg/( kg? h) infusion of dexmedetomidine, and control group received saline infusion.Serum TNF-αand IL-6 levels in two groups were compared at different time.Results After application of dexmedetomidine, the related indexes of hemodynamics in observation group, mean arterial pressure (MAP) and heart rate (HR) were more stable (P<0.05).Serum levels of TNF-αat the time of after induction, skin incision operation, 1h operation in observation group were (1.32 ±0.35), (1.42 ±0.54), (1.52 ±0.67), (1.57 ±0.63) μg/L, and were lower than those of control group, respectively (P<0.05).Serum levels of IL-6 at the time of after induction, skin incision operation, 1h operation in observation group were (69.64 ±13.54), (72.43 ±16.75), (76.32 ±16.93), (77.42 ±15.75) μg/L and were lower than those of control group, respectively (P<0.05).Conclusion The hemodynamics after application of dexmedetomidine in patients with neurosurgery operation is more stable, and the serum TNF-αand IL-6 are lower, so it contributes to the anti-inflammatory and has important clinical value.

Myopia is one of the three main school public health problems in China, the prevalence of which has been showing a rising and low-aging tendency in the past few decades. High myopia can cause several vision -threatening problems such as degenerative retinal conditions, cataracts, retinal detachment, and choroidal neovascularization. Its related complications are the leading causes of visual impairment and blindness, adversely impacting the afflicted individual's quality of life. Therefore, the prevention and control of myopia have become a common concern of family, school and health department. The purpose of this review is to summarize the progression of a variety of intervention methods used to prevent and control childhood myopia in recent years.

Objective: To investigate the correlation between the quality and climatic factors for Gentiana officinalis H. Smith from different producing area in Gansu province, China by grey correlation degree analysis combined with CRITIC-AHP weight ed evaluation. Methods: Contents of the total secoiridoid glycosides, total alcohol extracts and the metallic elements Ca, Fe and Zn were determined for the medicinal herb G. officinalis samples from 10 producing areas in Gansu, and their quality was estimated by the CRITIC-AHP weighted evaluation. The relationship between the quality and related climatic factors was then investigated by the grey correlation degree analysis. The climatic factors included the altitude, annual mean temperature, monthly mean temperature of Janu ary and July, annual mean relative humidity and the annual mean rainfall precipitation. Results : The content of the determined com ponents showed variation in the G. officinalis samples from the 10 different producing area. The altitude, annual mean temperature, monthly mean temperature of January and July, annual mean relative humidity and the annual mean rainfall all showed certain influ ence on the G. officinalis quality, and the biggest influence was the monthly mean temperature of July. Conclusion: The present re sults of the grey correlation degree analysis identifies the main climatic factors influencing the G. officinalis quality, which provides an experimental basis for the proper area selection and planting standardization for the G. officinalis production.

Gentianae Macrophyllae Radix is a Chinese medicinal material with unique efficacy and rich resources, which is widely distributed in northwest China. Gentianae Macrophyllae Radix contains a variety of chemical components, including iridoids, lignans, flavonoids, triterpenes, alkaloids, and other components, with anti-inflammatory and analgesic, liver protection, anti-virus, anti-tumor, immunosuppression, antihypertensive and other activities. Because of its various chemical components and wide range of pharmacological activities, it can be used as a kind of medicinal plant with great development and utilization value. With the great increase in the demand for Gentianae Macrophyllae Radix resources, the wild Gentianae Macrophyllae Radix resources are extremely shrinking. There are many medicinal sources of Gentiana Macrophylla Radix, and the medicinal parts are different, resulting in mixed sources of Gentianae Macrophyllae Radix. Not only the medicinal ingredients are unstable, the market chaos is also very serious, and the quality standard needs to be improved urgently. Based on the analysis of the present situation of Gentianae Macrophyllae Radix resources, chemical composition and pharmacological action, combined with the concept of quality markers, the quality markers of Gentianae Macrophyllae Radix were predicted and analyzed from the aspects of chemical composition and traditional medicinal properties, traditional efficacy, clinical efficacy, different compatibility and so on, in order to provide reference for the establishment of quality evaluation system of Gentianae Macrophyllae Radix.

OBJECTIVE: To describe and analyze the clinical characteristics of nasopharyngeal teratoma in infants and improve the levels of diagnosis and treatment to reduce misdiagnosis. METHOD: The clinical data of 11 cases of nasopharyngeal teratoma in infants were reviewed retrospectively and summarized the clinical features. After the preoperative examination of nasopharyngeal endoscopy and imaging (CT or MRI), all patients were under general anesthesia of nasopharyngeal teratoma surgical resection. Of them, 8 cases were used radiofrequency ablation, 3 cases used conventional power systems. RESULT: Eleven cases with neoformation were completely removed, the operation time was 5-15 min, blood loss was 1-5 ml, patients had no significant nasal obstruction, bleeding, eating nasopharyngeal regurgitation, breathing difficulties or other complications. No recurrence was found after 10 months to 9 years followed up. CONCLUSION The nasopharyngeal mass must be kept in mind in the differential diagnosis of breathing difficulties in infants, especially when it exists together with upper airway obstruction symptoms; The low-temperature radiofrequency ablation with endoscopic had the advantages of short time, precise control, less bleeding, clear operative field, little injury and pain, low recurrence, providing a new clinical treatments.
Catheter Ablation ; Child ; Child, Preschool ; Endoscopy ; Humans ; Infant ; Nasal Obstruction ; Nasopharyngeal Neoplasms ; surgery ; Nasopharynx ; pathology ; Neoplasm Recurrence, Local ; Retrospective Studies ; Teratoma ; surgery

OBJECTIVE: To analyze clinical features with benign paroxysmal positional vertigo (BPPV) and discuss the attentions in the canalith repositioning procedures. METHOD: A total of 76 male and female patients aged 80 and over with BPPV (elderly group) and 76 patients aged 60-65 years old with BPPV (older group) was retrospectively analyzed. RESULT: (1)Semicircular canal condition: in elderly group, posterior semicircular canal was involved in 72 cases, whereas the horizontal semicircular 4 cases. In older group, posterior semicircular canal was involved in 70 cases, whereas the horizontal semicircular and multiple canals in 5 cases and 1 case respectively. (2) Precipitating factors: precipitating factors of elderly were variety. Its closely related with emotion, infection, seasonal alternation surgery, and trauma. There were significant differences between the two groups (P<0. 05). (3) The symptoms of undergoing treatment and post-treatment: the duration of dizziness and carebaria were significant difference after canalith repositioning procedure treatment between two groups (P<0. 05), the duration of balance disturbance and symptoms of vegetative nerve functional disturbance like nausea and sweating were significant difference (P<0. 05). (4) Treatment and outcomes: the remission, partial remission rate were 34. 2 %, 81. 6% re- spectively, after the first or second time of repositioning treatment. The efficacy of repositioning treating at the first time was significantly different between two groups (P<0. 05). It was poor efficacy in elderly group. There is no difference in efficacy for repositioning treatment at the second or third time (P>0. 05). (5) The elderly always accompanied with other medical condition and had risk factors of cerebrovascular disease. The efficacy was not associated with the complication(P>0.05). However, it was most likely to overtreatment caused by emphasizing other medical conditions treatment. BPPV was easy to ignore and misdiagnose, meanwhile, delayed the diagnosis and increased the medical costs. (6) Many elderly were accompanied cervical spondylosis, lumbar spondylosis body stiffness and fear of vertigo which increased the difficulty of repositioning treatment. (7) Recurrence: we followed up 2 years after treatment. In older group, 11 patients (14. 5%)were relapsed. In elderly group, 29 patients (38. 2%) relapsed. There were significant differences between the two groups (P<0. 05). CONCLUSION There are various precipitating factors in elderly patients with BPPV, the most frequent precipitating factors were related to psychological factor and overfatigue. The symptoms of the patients attack BPPV was always mask with other diseases, but do not impact on the efficacy of Canalith repositioning at the first time; Even the efficacy of repositioning is poor at the first time, it's effective and safety after multiple treatments of repositioning; It prolonged the symptoms including carenaria, dizziness and nausea after treatment.
Aged ; Benign Paroxysmal Positional Vertigo ; complications ; diagnosis ; psychology ; therapy ; Dizziness ; Emotions ; Female ; Humans ; Male ; Masks ; Patient Positioning ; adverse effects ; methods ; Recurrence ; Retrospective Studies ; Semicircular Canals ; Spondylosis ; Vertigo