OBJECTIVE:To observe the clinical efficacy and safety of recombinant human interferon α2b (rhIFN α2b) combined with bozhi glycopeptides or thymopentin in the treatment of chronic hepatitis B (CHB).METHODS:Ninety HBeAg-positive CHB patients were selected from our hospital during Jan.2014-Jan.2015 and then randomly divided into group A,B,C,with 30 cases in each group.Group A was given rhINF α2b for injection (Pseudomonas) 5 million IU subcutaneously,qod;group B was additionally given Bozhi glycopeptides injection 4 mL added into 5％ Glucose injection 250 mL,ivgtt,qd,on the basis of group A;group C was additionally given Thymopentin for injection 2 mg added into 5％ Glucose injection 250 mL,ivgtt,qd,on the basis of group A.Three groups were treated for 24 weeks.The rate of ALT recovering to normal,negative rate of HBeAg,transformation rate of HBeAg/anti-HBeAg serum,negative rate of HBV-DNA and the decrease of HBsAg and HBV-DNA were compared among 2 groups after 4,8,12,24 weeks of treatment.The occurrence of ADR was recorded.RESULTS:After 4,8,12 weeks of treatment,there was no statistical significance in the rate of ALT recovering to normal,negative rate of HBeAg,transformation rate of HBeAg and the decrease of HBsAg among 3 groups (P＞0.05).After 4 weeks,negative rate of HBV-DNA in group B,C were significantly higher than group A;the decrease of HBV-DNA in group C were more significant than group A and B,with statistical significance (P＜0.05).After 8,12 weeks of treatment,the negative rate of HBV-DNA and the decrease of HBV-DNA in group B,C were significantly higher than group A,with statistical significance (P＜0.05);but there was no statistical signifi cance between group B and C (P＞0.05).After 24 weeks of treatment,there was no statistical significance in the rate of ALT recovering to normal,transformation rate of HBeAg,the decrease of HBsAg and negative rate of HBsAg among 3 groups (P＞0.05).The negative rate of HBsAg,negative rate of HBV-DNA and the decrease of HBV-DNA in group B,C were significantly higher than group A,with statistical significance (P＜0.05);there was no statistical significance between group B and C (P＞0.05).There was no statistical significance in the incidence of ADR among 3 groups(P＞0.05).CONCLUSIONS:rhIFN α2bcombined with bozhi glycopeptides or thymopentin shows good inhibitory effect on CHB,therapeutic efficacies of them are similar in the rate of ALT recovering to normal,but transformation rate of HBeAg,the decrease of HBsAg and negative rate of HBeAg.

OBJECTIVE: To explore the effects of serum containing Yiqi Xiaoji Recipe (YQXJR), a compound traditional Chinese herbal medicine for benefiting qi and removing stagnation, on cell line NKN-28 of gastric cancer. METHODS: The experimental SD rats were taken as the provider of the animal serum, and the serum was inactivated before the experiment. The serum was divided into high-dose, medium-dose, low-dose and blank serum groups based on whether the rats were given YQXJR and administration dosage. The inhibition rate was regarded as the observational index. RESULTS: The four groups of serum all had inhibitory effect on the growth of NKN-28 cells depending on the drug concentration. And there were significant differences among the experimental groups. High-dose, medium-dose and low-dose concentrations of serum all could inhibit the growth of NKN-28 cells with positive relations with the concentration and function time. CONCLUSION: YQXJR serum can inhibit the growth of NKN-28 cells depending on the drug concentration and function time.

ABCG2 is a member of the ATP-binding cassette (ABC) transporter family.The overexpression of ABCG2 is identified as one of the important mechanisms that limiting cellular accumulation of various compounds.With regard to its broad substrate spectrum including various anticancer drugs and environmental carcinogens,the function of ABCG2 is associated with multidrug resistance (MDR) and tumor development.ABCG2 as a target site to reverse MDR has been widely concered.

ObjectiveUnderstanding demand conditions for medical practice of the current medical schools nursing undergraduate and practical status of education,providing realistic and reliable basis for the reform of the nursing practice,and inquirying the practical measures of the teaching reform.MethodsAnalysing the 118 questionnaires issued by nursing students participated in medical practice and investigating the purpose of the nursing students wanting to achieve,the teaching form approved,the deficiencies proposed and overall satisfaction of teaching.ResultsThere is a considerable gap between nursing students' satisfaction,the strong demand,great interest of medical practice and current situation.ConclusionWe should concerne about the interns' strong demand for medical practice,pay attention to the teaching content,methods,objectives and organizational arrangements for many aspects of the construction and improvement.

Antibodies, Monoclonal ; therapeutic use ; Antibodies, Monoclonal, Humanized ; Antineoplastic Agents ; therapeutic use ; Antineoplastic Combined Chemotherapy Protocols ; therapeutic use ; Breast Neoplasms ; drug therapy ; metabolism ; pathology ; Carcinoma, Ductal, Breast ; drug therapy ; metabolism ; pathology ; Female ; Humans ; Receptor, ErbB-2 ; metabolism ; Trastuzumab

Adult ; Body Mass Index ; Child ; Humans ; Insulin Resistance ; Obesity ; complications ; Sleep Apnea, Obstructive ; etiology ; physiopathology

Hospital infection has become a global public health problem. The strain of anti-disinfectant, same as the antibiotic-resistant strain, will lead to the failure of the disinfection of the hospital, which will cause hospital infection. The bacterial resistance to disinfectants and resistance mechanism is not clear. Most of the studies focused on the cell research, the molecular research, non-physiological mechanism of resistance, in recent years, the selection pressure, outflow pump were studied. Currently, disinfectants abuse, improper handling and inadequate amount of disinfectant were considered as the main cause of the resistance development.

Objective To investigate and analyze nasal carriage of bacteria in medical staff and antibiotic susceptibility. Methods All study participants underwent swabbing of the anterior 1.5 cm of the nasal vestibule of both nares with a sterile swab,and did drug sensitivity test Apr.-Oct.,2007. Results In 41.08% of all samples (125/299),136 pathogenetic bacteria were isolated. Klebsiella pneumoniae,Staphylococcus aureus and Enterobacter aerogenes were the prominent bacteria,of which showed the resistance for more than three kinds of antibiotics were 35,accounting for 25.73% of all isolates,10 strains of Methicillin-resistant Staphylococcus (MRS) were detected. Conclusion The nasal carriage of bacteria and antibiotics resistance in medical staff in Tianjin should be paid attention to,for it may an important factor in the control of infection in hospitals.

Objective To investigate the effects of sulfur dioxide(SO2) pollution in different concentrations on activity of SOD,levels of GSH and MDA in heart and lung tissue of exercised rats,in order to elucidate the toxicological mechanism of SO2 pollution.Methods Forty-eight SD rats were randomly divided into eight groups:a rest group(RG),an exercise group(EG),a low pollution exercise group(LEG),a low pollution rest group(LRG),a moderate pollution exercise group(MEG),a moderate pollution rest group(MRG),a high pollution exercise group(HEG) and a high pollution rest group(HRG).All groups,except RG and EG,were exposed to SO2 with different concentrations(5 mg/m3,10 mg/m3,15 mg/m3),meanwhile exercised rats run in a motor-driven wheel at a speed of 8 m/min,2 h/d for 10 days.Rats were sacrificed at 24 h after treatment and the anti-oxidative enzymes activity and lipid peroxidation(LPO) levels were measured.Results Compared with RG,SOD activity of lung tissue significantly decreased in HEG,MEG,LEG(P

Aim To investigate the effects of breviscapine(Bre)on Na+,K+-ATPase activity of proximal tubule(PT) and its renal protection in early diabetic rats. Methods The rats were divided into three groups: diabetic model (DM group), Bre treatment (Bre group) and normal control (NC group). Rats were administrated with Bre(20 mg?kg-1?d-1, ip) in Bre group, with normal saline(ip) in DM and NC groups for four weeks after diabetes induction with streptozotocin (STZ 65 mg?kg-1, ip) in Bre and DM groups. The urine and blood samples were collected from two intra-ureteral cannulas and the heart, respectively, under anesthesia four weeks after diabetes induction. After one of the renal arteries being perfused, the renal cortex was incubated and PT segments were microdissected freehand under microscope before the Na+,K+-ATPase activity in the segments were assessed by liquid scintillation counter. The blood glucose, levels of creatinine in serum and in urine were assayed. The microalbumin , ?2-microglobulin(?2-MG) of urine and endogenous digitalis-like substance (EDLS) of serum were measured respectively by radioimmunoassay. Results The PT Na+,K+-ATPase activity in NC group was(959.11?117.35) pmolPi?mm-1?h-1, and that in DM group was significant higher for (1893.53?383.90) pmolPi?mm-1?h-1 than it(P