1.Comparing inhibition effects of four antihistamines on chemokines and cytokines expression of cultured nasal polyps in vitro
Journal of Third Military Medical University 2003;0(24):-
Objective To investigate the inhibition effects of different antihistamines on the expressions of chemokines and cytokines released by nasal polyps in vitro.Methods The fresh nasal polyps resected under endoscope from the patients admitted to our hospital were cultured with mizolastine,cetirizine,loratadine,fexofenadine,dexamethasone,or ciclosporin A respectively for 24 h,with the addition of histamine and arachidonic acid.The total RNA of polyps was obtained and purified with tripure isolation reagent.The mRNA expression levels of monocyte chemoattractant protein-1(MCP-1),monocyte chemoattractant protein-3(MCP-3),regulated on activation,normal T cell expressed and secreted(RANTES),eotaxin were analyzed by RT-PCR.The concentrations of interleukin(IL)-4,IL-5 and tumor necrosis factor-?(TNF-?)in cultured supernatant was detected by ELISA.Results The MCP-1 mRNA expression in nasal polyps treated by mizolastine was weaker than those by loratadine and fexofenadine;the MCP-3 mRNA expression by mizolastine was weaker than those by cetirizine and loratadine;the RANTES mRNA expression by mizolastine was weaker than those by loratadine and fexofenadine;the eotaxin mRNA expression by mizolastine was weaker than that by cetirizine(all P
2.c-Myc promoted migration and invasion of malignant melanoma B16-F10 cells through regulating PIK3 R3
Jianing YANG ; Fei DENG ; Ning PAN
Chinese Journal of Immunology 2017;33(1):66-71
Objective:To investigate the effect and the related mechanism of c-Myc on the proliferation,invasion and migration ability of malignant melanoma B16-F10 cells. Methods:We detected the expression of PIK3R3 in malignant melanoma and normal tis-sues. Efficiency of gene silencing of c-Myc and PIK3R3 was determined by qPCR and Western blot. We detected the proliferate ability of B16-F10 cells after silencing c-Myc and PIK3R3 using EdU assay. We detected the migration and invasion ability of B16-F10 cells after silencing c-Myc and PIK3R3 using wound healing assays and Transwell matrigel invasion assays. The expression of miR-199a after silencing c-Myc and PIK3R3 using qPCR. The expression of c-Myc and PIK3R3 was detected by qPCR after transfecting miR-199a mimics or miR-199a inhibitor. Dual-luciferase reporter assay system was used to detect miR-199a regulating c-Myc and PIK3R3. Results:Compared normal skin tissue,expression of PIK3R3 was significantly increased in malignant melanoma tissue;after silencing c-Myc and PIK3R3 gene,the proliferation,invasion and metastasis of melanoma cell line B16-F10 were significantly reduced;expression of miR-199a upregulated after silencing c-Myc and PIK3R3 genes, expression of c-Myc and PIK3R3 decreased after transfecting miR-199a mimics, expression of c-Myc and PIK3R3 upregulated after transfecting miR-199a inhibitor, dual luciferase reporter system test results revealed miR-199a can directly regulate c-Myc and PIK3R3 transcription activity. Conclusion: miR199a regulated the expression of c-Myc,then promote proliferation,migration and invasion in malignant melanoma cells.
3.Therapeutic Observation of Acupuncture plus Auricular Point Electroacupuncture for Post-stroke Depression and Its Effect on the Quality of Life
Fei WANG ; Wei PAN ; Yunfang LI
Shanghai Journal of Acupuncture and Moxibustion 2016;35(9):1033-1035
Objective To evaluate the clinical efficacy of acupuncture plus auricular point electroacupuncture and its effect on the quality of life (QOL).Method Ninety-eight patients with post-stroke depression were randomized into a treatment group and a control group, 49 cases in each group. The treatment group was intervened by acupuncture plus auricular point electroacupuncture, while the control group was by oral administration of Fluoxetine hydrochloride. The Hamilton Depression Scale (HAMD), Side-effect Rating Scale (SERS), and the World Health Organization Quality of Life Questionnaire-Brief Version (WHOQOL-BREF) were observed before and after intervention, and the clinical efficacies were also compared.Result The total effective rate was 89.1% in the treatment group versus 87.0% in the control group, and the between-group difference was statistically insignificant (P>0.05). Respectively after 2-week, 4-week, and 8-week treatment, the HAMD scores were significantly different from that before intervention in the treatment group (P<0.05). Respectively after 2-week, 4-week, and 8-week treatment, the HAMD scores were significantly different from that before intervention in the control group (P<0.05). There were no significant differences in comparing the HAMD scores between the two groups after 2-week, 4-week, and 8-week treatment (P>0.05). The SERS scores in the treatment group were significantly different from that in the control group after 4-week and 8-week treatment (P<0.05). In the treatment group, the SERS score after 8-week treatment was significantly different from that after 4-week treatment (P<0.05). After treatment, the scores of general health domain 1 and 4, physical health domain, psychological health domain, and environment domain from WHOQOL-BREF were significantly changed in both groups (P<0.05). After intervention, the physical health domain score of the treatment group was significantly different from that of the control group (P<0.05).Conclusion Acupuncture plus auricular point electroacupuncture is an effective approach in treating post-stroke depression, and it can improve the quality of life of the patients.
4.Distribution and content of transforming growth factor-β1 and vascular endothelial growth factor in each layer of concentrated growth factors
Fei CHEN ; Shaoxia PAN ; Hailan FENG
Journal of Peking University(Health Sciences) 2016;48(5):860-865
Objective:To investigate the distribution and content of transforming growth factor-β1 (TGF-β1)and vascular endothelial growth factor (VEGF)in concentrated growth factors (CGF)gel, and to clarify the difference among different layers of CGF.Methods:Venous blood samples were col-lected from 6 healthy volunteers to prepare CGF.The distribution,integrated optical density (IOD)and average optical density (AOD)of TGF-β1 and VEGF in CGF gel and red blood cell (RBC)layer were measured using immunohistochemistry.The concentrations of TGF-β1 and VEGF in the supernatant se-rum at baseline and the CGF releasate after 1 day were evaluated with enzyme-linked immunosorbent as-says.Results:Abundant TGF-β1 and VEGF were concentrated in CGF gel.However,only a little could be found in polykaryocytes and sporadic platelets in RBC layer.Platelets and leukocytes were concentra-ted in between the two layers with high expression of TGF-β1.The concentrations of TGF-β1 and VEGF in the CGF releasate(55 236.78 ±3 686.34),(610.99 ±148.81)ng/L were significantly higher than those in the supernatant serum (20 710.20 ±4 523.14),(335.20 ±51.69 )ng/L (P <0.001 ). Conclusion:CGF contains high quantities of TGF-β1 that can promote new bone formation and tissue healing.We suggest that CGF gel should be used right after being prepared.Supernatant serum and the area between CGF gel and RBC layer could also be mixed with bone substitute materials.
5.The expression of CD1a and E-cadherin in condyloma accuminatum tissues and the ultramicrostructure of Langerhans cell
Yongzheng PAN ; Fei WANG ; Dan LUO
Chinese Journal of Immunology 1985;0(02):-
Objective:To investigate the changes in number,morphology and function of Langerhans cells and the molecular mechanisms in the tissues of condyloma accuminatum.Methods:CD1a,E-cadherin were measured by streptavidin peroxidase method and reverse transcriptase PCR in 26 CA lesions and 10 normal controls.Ultramicrostructure of LC was observed by transmission electronic microscope(TEM).Results:In CA tissues,the number of CD1a+ LC decreased(10.66?11.71,P
6.Rapid diagnosis of acute dyspnea using the combined test of NT-pro-BNP and left ventricular ejection fraction
Xufeng YANG ; Aihua FEI ; Shuming PAN
Clinical Medicine of China 2010;26(9):933-937
Objective To investigate the value of N-termind pro-B-type natriuretic peptide(NT-pro-BNP)in differential diagnosis of dyspnea in emergency department, and to investigate the rapid diagnosis cutoff of dyspnea due to acute congestive heart failure. Methods Ninety. one cases of dyspnea in emergency department recruited from January to June ,2008 were divided into two groups: acute cardiac dyspnea group and none acute cardiac dyspnea group. To evaluate the value of different parameters in differential diagnosis of dyspnea in emergency department and analysis the area under the receiver-operating characteristic of different parameters for the diagnosis of acute cardiac dyspnea. To achieve the best cutoff of different parameters for the diagnosis of dyspnea due to acute congestive heart failure finally. Results Among two groups, NT-pro-BNP (acute cardiac dyspnea vs. none acute cardiac dyspnea,6203.50 ng/L vs. 1410.00 ng/L,P < 0.01), Troponin Ⅰ (acute cardiac dyspnea vs. none acute cardiac dyspnea,0.12 μg/L vs. 0.03 μg/L,P <0.01) ,left ventricular ejection fraction(acute cardiac dyspnea vs. none acute cardiac dyspnea,46.25% vs. 65.60%, P < 0.01), left atrial diameter (acute cardiac dyspnea vs. none acute cardiac dyspnea,42.75 mm vs. 36.00 mm,P <0.01) had significant difference. NT-pro-BNP at cutoff of ≥3715 ng/L was highly sensitive and specific for the diagnosis of acute cardiac dyspnea. Receiver-operating characteristic analyses demonstrated that NT-pro-BNP and left ventricular ejection fraction was the best diagnostic indices of acute cardiac dyspnea. The area under the receiver-operating characteristic curve of Nt-pro-BNP was 0.828 ± 0.045 (P < 0.01),and left ventricular ejection fraction was 0.829 ± 0.049 (P < 0.01). Correlation analysis showed that NT-pro-BNP was correlated with left ventricular ejection fraction (P < 0.01). The combined test of NT-pro-BNP and left ventricular ejection fraction was performed. Specificity increased to 96.50%, total consistent rate increased to 83.50% ,positive predictive value increased to 91.30%, positive likelihood ratio 17.60, faulse diagnostic rate decreased to 3.50%. Conclusions NT-pro-BNP examination in emergency department was helpful to rapid differential diagnosis of dyspnea. It helped to differentiate the patients with acute congestive heart failure and none acute congestive heart failure causes of dyspnea.
7.Effect of combination of paclitaxel and resveratrol on human gastric carcinoma cell strain MGC803 in vitro
Hongming PAN ; Hongxin FEI ; Xiaoyi HUANG
China Journal of Traditional Chinese Medicine and Pharmacy 2006;0(09):-
Objective: To evaluate effect of combination of PA and RES on human gastric carcinoma cell MGC803 in vitro.Methods :The effects of PA and RES were measured by MTT assay.Morphous of cell was observed by light microscope.Flow cytometry was used for MGC803 cell cycle analysis.Results: PA significantly inhibited the growth of MGC803 cell in a dose and time dependent way(P
8.Management of nosocomial consumables in supermarket style based on PowerBuilder
Jun FEI ; Xiaodong PAN ; Meijuan JIN
Chinese Medical Equipment Journal 2003;0(12):-
Objective To realize management of nosocomial consumables in supermarket style by developing bar code input and output modules.Methods Based on No.1 Military Medical Project and Consumables Management System,source codes were extracted and bar code input and output modules were added into original system.Results With the bar code modules,the actual work flow of this system was simplified to be three steps: consumables input by scanning bar codes;scan statistics;consumables output by scanning bar codes.Conclusion A lot of disadvantages in original workflow are removed such as overmuch procedures in workflow,frequent occurrence of errors,high labor repetition,low work efficiency,etc.work efficiency and accuracy are greatly improved and supermarket style management of nosocomial consumables is realized.
9.Management system for war readiness material in hospital based on PowerBuilder
Jun FEI ; Xiaodong PAN ; Yuanlin JIANG
Chinese Medical Equipment Journal 1989;0(01):-
Objective To develop a set of software to manage war readiness material, such as the medical equipment, the medical material of medicine(consumables), the instrument, etc. Methods Based on the platform of PowerBuilder, management modules for equipment, consumables, medicine and others material were designed. Results Material data could be modified, added, reduced and queried in the corresponding module. Conclusion Changing the mode of manual management, the management level is raised, the waste is reduced and equipment can be repaired promptly.
10.Design and Implementation of Management System for Hospital Combat Readiness Based on PowerBuilder
Jun FEI ; Yuanlin JIANG ; Xiaodong PAN
Chinese Medical Equipment Journal 2003;0(10):-
Objective To develop a software to turn the management of combat readiness into information -based management in computer. Methods Based on the Platform of Pb10.0, management modules were designed and database was developed. Results In the corresponding module, operations such as modifying, inquiry or increase could be performed. Conclusion The management level is raised, the waste of commodity is reduced and the equipment in good condition is ensured.