10.3969/j.issn.1000-3606.2013.06.022

Objective To systematically analyze therapeutic effects of extracorporeal photopheresis (ECP) on refractory steroid-resistant,-dependent or-intolerant graft-versus-host disease (GVHD),to compare remission rates of skin GVHD in detail.Methods Databases were searched for studies concerning remission rates of GVHD after the treatment with ECP,and relevant data were extracted and pooled by using meta-analysis with STATA 12.0 software.Results A total of 22 literatures were reviewed,including 787 patients with refractory GVHD.Meta-analysis showed that the pooled complete remission (CR) rate and overall remission (OR) rate were 57％ (95％ CI:47％-66％) and 79％ (71％-86％) respectively in patients with acute GVHD,27％ (21％-33％) and 63％ (58％-69％) respectively in patients with chronic GVHD,77％ (71％-82％) and 87％ (82％-91％) in patients with acute skin GVHD,as well as 39％ (30％-48％) and 70％ (63％-77％) in patients with chronic skin GVHD.Conclusion ECP is an effective therapy for refractory GVHD,especially for skin GVHD.

Aged, 80 and over ; Female ; Fracture Fixation ; adverse effects ; Humans ; Male ; Postoperative Complications ; etiology ; Radius Fractures ; surgery

Objective To explore the effect of early screening and psychotherapy on post stroke depression. Methods 80 patients with stroke at first onset were assessed with Hamilton Rating Scale for Depression (HAMD-17). Patients with depression were divided into control group (n=21) and intervention group (n=21). Both groups received rehabilitation therapy and the intervention group received psychotherapy in addition. They were assessed with HAMD again 2 months after treatment. All of them were evaluated with modified Barthel index (MBI). Results 52.5% patients had post stroke depression. The score of HAMD-17 was lower in the intervention group than in the control group (P<0.01). There was no significant difference in MBI between 2 groups after treatment (P>0.05). Conclusion Early screening and psychotherapy can facilitate the recovery of patients with depression after stroke.

Adult ; Anti-Bacterial Agents ; adverse effects ; Female ; Humans ; Laryngeal Diseases ; etiology ; microbiology ; Mycosis Fungoides ; etiology ; Prescription Drug Misuse ; Substance-Related Disorders ; complications ; Young Adult

Objective To investigate the mycoplasma pneumoniae(MP) infection of the respiratory tract infection in children,the macrolide-resistant situation and resistance mechanism of MP. Methods The cultured throat swab specimens were obtained from 80 pediatric outpatients with respiratory tract infection from Dec.2008 to Mar.2009 in Beijing friendship hospital.The 23S rRNA gene of throat swab specimens and positive-cultured specimens were amplified using nested-PCR,and the products were further verified by electrophoresis and DNA sepuencing,which were collected from the outpatients.The specimens were divided into 2 groups depending on the findings of the gene sequencing whether they had gene mutation:sensitive and resistance group.The DNA sequence of samples were compared to the sequence of MP reference strain in genbank in order to findout MP drug resistant gene.The differences in macrolids therapy were investigated between 2 groups before the throat swab obtained.The drug resistance rates were compared between outpatients and inpatients. Results Thirty-two throat swab specimens were proved to be MP by direct nested-PCR,and 8 throat speeimens were proved to be MP by isolation and culrures.Total 33 cases(including 1 was positive-culture but nagative-direct PCR) were proved to be MP positive.Sixteen were identical to the M129 standard sequence,and 17 had point mutation in gene of 23S rRNA V region.Ten had A to G mutation at position 2063,3 had A to G mutation at position 2064,2 had A to G mutation at position 2067,1 had G to A mutation at position 2062,1 had A to T mutation at position 2063.There was no significant difference between the sensitive and resistance group in whether had macrolids before the throat swab obtained(P =0.909).And there was no significant difference in MP drug resistance rate between outpatients and inpatients(P =0.459). Conclusions The major mutation were A2063G and A2064G,and A2063T,A2067G,G2062A were newly found mutation points which were possibly related to macrolids resistance.

Full text of literatures on randomized clinical trials (RCTs) of intracoronary infusion of autologous bone marrow-derived stem cells (BMSCs) transplantation in patients with acute myocardial infarction (AMI) published at home and abroad were systematically retrieved and reviewed from PubMed database and the China Full Text Journal database to assess its efficacy and safety. Fifteen RCTs were included in a recta-analysis with RevMan version 5. 0 software after strict and standard screening. Results showed that global left ventricular ejection fraction (LVEF) in the patients with BMSCs transplantation improved significantly ( P < 0. 01 ), but no obvious change in their left ventricular end-diastolic volume (LVESV) was observed. Occurrence of end-stage cardiovascular events also decreases significantly ( OR =0. 51, 95% CI =0. 36-0. 72) in patients with AMI.

Objective To observe the histopathological features of early skin lesions and serum inflammatory mediators changes in the diabetes mellitus(DM) rats.Methods A total of 50 Wistar rats at 8 to 10 weeks of age were divided into normal control group(C group,n=25) and diabetic group(DC group,n=25).The rats of DC group were given a high-glucose and high-fat diet for 2 weeks to induce insulin resistance and then an intraperitoneal injection of 35 mg/kg streptozotocin.All 25 rats were identified to be diabetic models with their fasting blood glucose over ≥7.0 mmol/L.Four rats of C group or DC group were killed to take the skin tissues in the 4th,8th and 12th weekend.In the 16th week,the heart blood samples of the left 13 rats of C group and 10 rats of DC group were collected to measure TNF-?,IL-6 and C-RP levels,and the skin samples were also taken for pathological observation and full-thickness and dermal thickness measurement.Results In the 16th week,the TNF-?,IL-6 and C-RP levels of DC group were significantly higher than the normal group(P

Objective To investigate the effects of rosiglitazone on serum levels of tumor necrosis factor-? (TNF-?),interleukin-6 (IL-6),C-reactive protein (C-RP) and superoxide dismutase (SOD),and early skin changes in the diabetic rats.Methods The rats were divided into normal control group (C group,n=13),diabetic control group (DC group,n=13),diabetes and insulin treatment group (DI group,n=13),diabetes and rosiglitazone treatment group (DR group,n=13).The diabetic rat models were established with high-fat diet for 2 weeks followed by intraperitoneal injection of 35 mg/kg streptozotocin injection,the rats were idenfied as diabetic when their fasting blood glucose (FBG) over 7.0 mmol/L.The living rats were given corresponding treatment,subcutaneous injection of 1 to 2 U/d insulin,intragastric injection of 5 mg/(kg?d) rosiglitazone,or sterile water.Heart blood samples of rats from each group were collected to measure TNF-?,IL-6,C-RP and SOD levels in the 16th weekend.Also the skin was taken for pathological observation and full-thickness as well as dermal thickness measurements.Results The serum levels of IL-6,TNF-? and C-RP in diabetic group were significantly higher than those in normal group (P

Objective:To prepare DEET-ethylcellulose microsphere(DEET-EC) and observe its properties to retard volatilization of DEET. Methods: DEET-EC was prepared with solvent evaporation method. DEET was dissolved in CH 2Cl 2 with EC served as dispersed phase and 1% PVA water solution as continuous phase. The dispersed phase was added into the continuous phase with stirring rate at 1 000 r/min. The stirring rate was changed into 600 r/min after 30 min, and was kept until CH 2Cl 2 was entirely volatilized. After being watered, precipitated and lyophilized for 12 h, DEET-EC was derived, and the shape was observed with electron microscope. The particle size distribution was detected in 500 microspheres with optical microscope. HPLC method was established to determine the embedding ratio and loaded ratio of DEET-EC microsphere. Chromatograph conditions: Diamonsil ODS column (150 mm?4.6 mm), CH 3OH∶H 2O=65∶35 as mobile phase at 1 ml/min, the detected wavelength 210 nm. Results: The DEET-EC was egg-white and had spherical shape. Almost 90% of the MS distributed in 30-70 ?m, while ( ar ) and ( v ) were 49.6 ?m and 51.2 ?m, respectively. The loading ratio was 18.7% and the embedding ratio was 56.1%(n=6). Conclusion: The solvent evaporation method is convenient and simple to prepare DEET-EC microsphere.