Biology-related disciplines in medical school of comprehensive university are cur-rently confronted with some difficulties, including unclear position and indistinguishable characteris-tics. In this paper, we proposed that biology-related disciplines in medical school of comprehensive university should center on disease research, emphasize medical feature and give full play to its tech-nical advantage thus to provide technical support for basic medicine and clinical medicine. Mean-while, sources of multiple disciplines should be effectively integrated by breaking through the limitation of discipline and administrative system. An interdisciplinary molecular medicine platform was built up for researching and teaching.

MicroRNA ( miRNA) is a family of 19 - 24 nucleotides small non - coding RNA in eukaryotic organisms , which can regulate genes at the post - transcriptional level. miRNA has been shown to be involved in a variety of cellular processes such as development, proliferation, differentiation, apoptosis etc, and is closely related to the oncogene-sis. Recent study indicates that several miRNAs play an important role in initiation and progression of hematological malignancies. miRNA expression profiling has identified signatures associated with diagnoses, prognoses and therapy of hematological malignancies. The review discusses miRNA and its biogenesis, functional mechanisms and potential role in the diagnosis , prognoses and therapy of hematological malignancies.

Normally, pancreatic islet?-cell mass is regulated by?-cell replication, neogenesis, apoptosis and cell size.?-cell mass in diabetic patients is conspicuously less than that in normal persons. Decline of?-cell mass is mainly caused by increased?-cell apoptosis. The change of?-cell mass is clinically becoming more and more important in regard to the progression of diabetes mellitus.

AIM: The effective antisense sequences targeted VEGF mRNA with computer software would be screened and designed, and effect of them on growth K562 cells and protein expression of VEGF were studied with experiments. METHODS: Seven antisense sequences were selected and synthesized, which consisted of 18-20 deoxynucleotide acid and were modified with phosphorothioate, according to principle of low free energy of overall △G 37 Overall. Cell growth was assayed by trypan blue dye exclusion assay and level of VEGF protien in the media was determined by ELISA. RESULTS: Six of seven sequences were capable of inhibing growth of K562 cells and downregulating the VEGF protein expression significantly, compared with Scrambed control group. It was found that there was a close correlation between low level of overall △G 37 and antisense effectiveness ( r =0 887, P

Objective To investigate the efficacy of auricular plaster therapy for post-laparoscopic cholecystectomy nausea and vomiting. Methods Two hundred and forty laparoscopic cholecystectomy patients were randomly allocated to treatment and control groups, 120 cases each. Both groups received routine nursing care. In addition, the treatment group received auricular plaster therapy. The incidence of nausea and vomiting was observed in the two groups.Results The incidence of nausea and vomiting was 46.7% in the treatment group and 60.8% in the control group; there was a statistically significant difference between the two groups (P<0.05).Conclusion Auricular plaster therapy can relieve postoperative nausea and vomiting in laparoscopic cholecystectomy patients.

Cricoid Cartilage ; surgery ; Humans ; Laryngectomy ; methods ; rehabilitation ; Larynx ; Recovery of Function

[Objective]To investigate the effect of atlanto-axial pedicle screw plate and fusion in treatment of upper cervical injuries. [Methods]Fifty-six cases of upper cervical spine injuries were treated with atlanto-axial pedicle screw plate and internal fixation,with total 200 screws,from March 2000 to September 2007.There were 36 males and 14 females,with an average age of 41.52 years(ranged,20~65 years).There were 23 cases of type II odontoid process fracture,12 cases of odontoid process nonunion,15 cases of transverse ligament injury.[Results]Greater occipital nerve pain occurred in 4 cases postoperatively and fully recovered after treatment 1 month later.The lateral cortical bone was penetrated by screws in 2 cases,without spinal cord or vertebral artery injury.Forty-eight cases were followed up for an average of 27.56 months(ranged,25~60 months).Bone fusion was achieved in all cases,without plate or screw broken.X-ray showed complete replacement of atlas and odontoid process of axis fracture.CT showed good position relation between screw and vertebral artery or spinal cord.According to JOA classification,excellent result was achieved in 34 cases,good in 13 cases,fair in 2 cases,poor in 1 case,with the good-to-excellent rate of 94.00%.[Conclusion]Atlanto-axial pedicle screw combined with plate fixation system is effective in treatment of upper cervical injuries.It can significantly improve the biomechanical stability of atlantoaxial joint,with high bone graft fusion rate.

Combined Modality Therapy ; Humans ; Stomach Neoplasms ; therapy

Objective To investigate the expression of brain-type glycogen phosphorylase(GPBB)as a new biomarker in colorec-tal carcinomas,to understand its expression timing in colorectal neoplasia and its relation with the clinical pathological parameters, and to compare it with the P53 gene.Methods 40 specimens of colorectal carcinoma and 18 specimens of colorecpal neoplasia were selected.The expression of GPBB and P53 in colorectal tumor was investigated by self-made specific anti-human GPBB antibody and P53 antibody.Results The positive expression rate of GPBB in colorectal carcinoma was 80%,which was significantly higher than that in colorectal carcinoma,moreover its expression in moderate and severe atypical hyperplasia adenoma and papillary adenoma was earlier than P53 gene expression.Conclusion GPBB is a enzyme expressed in carcinoma and precancerous lesion,therefore it may be taken as early biomarker enzyme for predicting the occurrence of colorectal carcinoma.

Objective: To investigate the changes of hemodynamic parameters by transesophageal doppler echocardiography (TEE) and pulmonary artery catheter (PCA) before and after volume administration in patients receiving off-pump coronary artery bypass (OPCAB) surgery, to analyze the correlation between the two methods, and to discuss the value of TEE in monitoring the preload during OPCAB. Methods: Twenty patients who were to receive OPCAB were enrolled in the present study. TEE was used to determine the mitral valve flow (MVF). Simultaneously, the central vein pressure (CVP), pulmonary capillary wedge pressure (PCWP), pulmonary artery pressure (PAP), mixed venous oxygen saturation (SvO2), cardiac output (CO), cardiac index (CI), and stroke volume (SV) were examined during OPCAB by PCA. The above parameters were recorded before and immediately after volume infusion (5 ml/kg, in 10 minutes). Patients with SV increased by > 10% were taken as effective responders. Pearson correlation analysis was used to examine the relationship of SV with the results of TEE and PAC. Results: There were 14 effective responders after the first volume load, and only 6 after the second infusion. Compared with the baseline values, PCWP of all the patients, and CO, CI and SV of the effective responders increased significantly after volume DOI: 10.3724/SP.J.1008.2009.01393 loading (P<0.05); the values of MVe, MTIe, and VTIe+a also increased significantly after volume loading (P<0.05); and there were no significant changes in MVa, VTIa, MVedt values after volume loading. The results of correlation analysis of SV with other parameters were, MVe (r=0.36, P<0.05), VTImve (r=0.63, P<0.05), VTIe/VTIa (r=0.67, P<0.01), VTIe+a(r=0.77, P<0.01), and PCWP (r=0.35, P<0.01). SvO2 was increased after infusion (P<0.05). Conclusion: The relationship of TEE parameters with SV is variable. There is a significant positive correlation between SV with VTIe and VTIe+a; and VTI can be used to evaluate the preload of left ventricular, which should be further studied to guide clinical medication.