Objective:To establish an HPLC method for the determination of two components and the preservative in compound dextromethorphan hydrobromide syrups. Methods:An Agilent Zobax SB-C18 column(250 mm × 4. 6 mm,5 μm) was used with meth-anesulfonic acid solution (adding 4. 8g methanesulfonic acid and 10ml triethylamine into 750ml water,and adjusting the pH value to 3. 5 by phosphoric acid)-acetonitrile (75∶25) as the mobile phase at the flow rate of 1. 0 ml·min-1 and 280nm as the detection wave-length. Results:The calibration curve was linear within the range of 102-1 025μg·ml-1 for guaifenesin,15-619μg ·ml-1 for dextro-methorphan hydrobromide and 10-407μg·ml-1 for benzoic acid. The average recovery of guaifenesin, dextromethorphan hydrobromide and benzoic acid was 100. 0% (RSD=0. 35%), 100. 1%(RSD=0. 77%)and 100. 8%(RSD=0. 49%), respectively. Conclusion:The method is simple,rapid and accurate,and suitable for the quality assessment of compound dextromethorphan hydrobromide syrups.

Objective To solve the conflict between data security and sharing in hospital so that patients can get their clinical data through result query service system.Methods Advanced secure data-exchange technology was used to realize network isolation.Integrated result query service system adopted the embedded system as hardware and the embedded LINUX operation system as software.Results Through LAN query module,internet query module and the secure data-exchange module,the design of HIS multimedia query system was realized.Conclusion Via the application of HIS integrated query platform,the design of the hospital secure data exchange is proved to be feasible,reliable and practical.

The factors influencing the integration of MOOC and information literacy education in academic libraries were divided into objective factor, subjective factor and social factor according to their connotation and significance. Certain measures for their integration were proposed, such as strengthening network construction, perfecting literacy education systems, and carrying out systemic training.Integration of MOOC and information literacy education in academic libraries can improve information service, promote wide application of MOOC and information literacy ed-ucation in academic libraries.

Objective: To exploration the self-efficacy, self-esteem, and positive/negative affect and the re-lationships among them in Chinese adolescents. Methods: The Rosenberg's Self-Esteem Scale, Generalized Self-Ef-ficacy Scale, and Adolescents Positive/Negative Affect Scale were administrated to 2247 middle school students sampled from 16 schools from 6 districts. Results: Self-esteem tended to increase as grade [(2.91±0.38), (2.93±0.41), (3.01±0.41), (2.98±0.44), (3.04±0.41), (3.17±0.42)]. Boys had higher self-efficacy [(2.74±0.39)vs.(2.68±0.40), P <0.001]. Two senior grades had higher self-efficacy than other 4 grades (P<0.05). Girls had higher positive and negative affect [(2.77±0.67)vs.(2.68±0.67), (2.22±0.70) vs.(2.13±0.68), Ps <0.05]. Junior grade one had the highest positive effect (2.85±0.62), grade one and two in high school had more negative effect than other 4 grades (2.26±0.71, 2.25±0.73).Self-efficacy and self-esteem were positively correlated with positive affect (r=0.24, 0.36), negatively correlated with negative af-fect (r=-0.17, -0.32) ; self-esteem significantly mediated the relationship between self-efficacy and positive/negative affect (Z=13.00, -13.07) . Conclusion: Self-esteem tends to increase as grade, boys have higher self-efficacy than girls, and girls have higher positive and negative affect than boys. Self-esteem mediates the rela-tionship between generalized self-efficacy and positive/negative affect in Chinese adolescents after controlling the effects of gender and grade.

Objective To study the psychological intervention combined with hydrotalcite in treatment of gastroesophageal reflux disease clinical effect.Methods 100 patients with gastroesophageal reflux in our hospital from February 2015 to October 2016 were selected as the research object in this study.They were randomly divided into the control group and the experimental group, each group was divided into two groups.The control group of hydrotalcite in treatment of chewing, oral.The patients in the experimental group received psychological intervention on the basis of hydrotalcite treatment, strengthen the communication with patients, patients understand the inner confusion, eliminate the patient anxiety and discomfort, improve the treatment compliance of patients.Comparative analysis of the experimental group and control group of patients with treatment effect.Results After treatment, 50 patients in the experimental group, the total effective number of cases of clinical symptoms was improved in all cases, the effective rate of treatment was 92%, and the number of effective cases of electronic gastroscope mucosal repair was 47 cases, the effective rate was.In the control group of 50 cases, the total effective number of cases of clinical symptoms was improved in 40 cases, the effective rate of treatment was 80%, and the number of effective cases of electronic gastroscope mucosal repair was 41 cases, the effective rate was about 82%.The effective rate of the experimental group was significantly higher than that of the control group(P<0.05).Conclusion Psychological intervention combined with hydrotalcite in treatment of gastroesophageal reflux disease has good clinical effect, can improve the therapeutic effect in a certain extent, with further clinical promotion and application significance.

Objective To investigate the clinical value of D5F3 and Survivin in patients with non-small cell lung cancer. Methods 100 patients with non - small cell lung cancer admitted in our hospital from January 2016 to June 2017 were examined by immunohistochemical method, to analyze the clinical value of D5F3 and Survivin antibody in non - small cell lung cancer (NSCLC). Results The coincidence rate of D5F3 was (20％), (++) was 33.33％, and the coincidence rate was (87.50％), and the difference was statistically significant (P<0.05). The positive rate of Survivin protein expression was correlated with the differentiation degree of non-small cell lung cancer, TNM stage and lymph node metastasis (P<0.05). Conclusion D5F3 has high accuracy, sensitivity and specificity, and has important clinical diagnostic value; The detection of Survivin expression is helpful for clinical differentiation, clinical staging and lymph node metastasis in patients with non-small cell lung cancer.

Objective To establish the quality control standard for Anzhongtongbian capsules. Methods Radix Et Rhizoma Rhei, Radix Paeoniae Alba and Cortex magnoliae officinalis in Anzhongtongbian capsules were identified by TLC method. The content of emodin was determined by HPLC. Results The Radix Et Rhizoma Rhei, Radix Paeoniae Alba and Cortex magnoliae officinalis in Anzhongtongbian capsules could be identified by TLC. The linear range of emodin was 0.08～0.24 mg (r =0.9999). The average recovery was 98.86% and RSD=1.11% (n =5). Conclusion This text method is simple and dependable, the exclusive is strong, good in reappearance, and available for the quality control of the preparation.

Pediatric blood purification requires reliable access to the circulation.Central venous catheters play an important role in the delivery of pediatric blood purification.A central venous noncuffed,nontunneled catheter is the best choice for short-term(less than 3 weeks) blood purification.A cuffed,tunneled catheter is preferable to long term(more than 3 weeks) blood purification.However,there are many complications associated with central venous catheters,such as catheter-induced thrombosis,catheter-related infection,and central vein stenosis.This article reviews the prevention and treatment of complications most frequently occurring with central venous catheters.

Using calmodulin dependent cyclic neucleotide phosphodiesterase and dansyl-labeled calmodulin,honokiol's action on calmodulin is studied. It is found that honokiol inhibits the activity of cyclic neucleotide phosphodiesterase induced by stimulus of calmodulin. With the increasing of calmodulin concentration, the IC50 value was increased. In the presence of Ca2+, honokiol reduces the fluorescence intensity of dansyl-calmodulin, and makes the spectrumpeaked red. The results indicate that honokiol can combine with calmodulin in the presence of Ca2+ and antagonise the effect caused by calmodulin's stimulation upon the phosphodiesterase. In addition , honokiol may exert its effect on the basal activity of calmodulin dependent cyclic neucleotide phosphodiesterase.

Objective To analyze the effect of calcitonin combined with alfacalcidol on clinical outcome,lumbar vertebrae bone mineral density and safety in patients undergoing internal fixation of humeral shaft fracture.Methods 80 patients with humeral shaft fracture treated with internal fixation in Xunwu county people's hospital from June 2010 to June 2016 were selected,and randomly divided into control group and observation group,40 cases in each groups.The control group was given calcium carbonate D3 tablets and functional exercise therapy,the observation group treated calcitonin combined with alfacalcidol on the basis of control group,analysis and comparison the clinical effects,lumbar spine BMD and safety.Results Compared with before treatment,serum calcium level of two groups increased significantly at treatment 2 weeks and 3 weeks(P<0.05);The control group BMD of lumbar spine was significantly increased in treatment 12 weeks(P<0.05),the observation group BMD of lumbar spine was significantly increased in treatment 4 weeks and 12 weeks(P<0.05).Compared with control group,the level of serum calcium in observation group was higher at treatment 2 weeks and 3 weeks (P<0.05),BMD of lumbar spine was higher at treatment 4 weeks and 12 weeks(P<0.05);The total adverse reaction rate of observation group was 10%,the difference was not statistically significant to control group 15%.Conclusion The combination of calcitonin and alfacalcidol treatment of internal fixation of humeral shaft fractures in patients with significant results, can improve serum calcium levels in patients with and improve bone mineral density, and less adverse reactions.