Objective:To express the recombinant protein of the hp1188 gene of Helicobacter pylori NCTC 11637 in order to lay a foundation for the study on adhesion. Methods:The hp1188 gene was amplified from NCTC 11637 chromosomal DNA by PCR.The PCR product was inserted into the expression vector pQE-30.The recombinant vector pQE30-hp1188 was identified by DNA sequencing and transformed to E.coli DH5? for expression under IPTG induction.The recombinant protein was purified by Ni~(2+)-NTA agarose and identified by SDS-PAGE and western blot. Results:The sequence of hp1188 gene amplified was about 810 bp with a 98% sequence homology with hp1188 genes in GenBank. SDS-PAGE determined that the molecular weight of its expression products was 30 600 Dalton. The expression products of E.coli DH5? lyzate was about 50% of total soluble proteins,and the pellet also had expressed. Its purity was over 90% after purified with Ni~(2+)-NTA agarose and Western blot showed that the recombinant protein possesses fine binding capacity with the rabbit anti-H.pylori. Conclusion:hp1188 gene was successfully cloned and highly expressed in E.coli DH5?.

The main pathology of wet age-related macular degeneration (AMD) is the development of choroidal neovascularization( CNV),and the critical role of VEGF in the pathogenesis of CNV is well established.Ranibizumab is a fragment of a humanized and monoclonal antibody.It can binds to VEGF,thereby,inhibiting the angiogenesis.Some clinical trials have proved the clinical efficacy of ranibizumab via intravitreal injection for wet AMD.On the other hand,ranibizumab is well tolerated for wet AMD patients after administration and is not associated with a clinically significant risk of ocular or systemic adverse events.The large-scale clinical trials showed that its principal ocular adverse event occurred in ＜4％ of ranibizumab-treated patients,including the transient elevation of intraocular pressure and mild ocular inflammation.It appears unlikely that application of ranibizumab does not increase the risk of vascular event significantly.Less-frequent injections on an as-needed schedule based on monthly monitoring may have the most optimal risk/benefit ratio.This article summarized the recent clinical trials of ranibizumab and offered high-quality evidences for the safety evaluation of ranibizumab treating wet AMD.

Age-related macular degeneration(AMD)causes severe vision loss due to the development of choroidal neovascularization(CNV).The critical role of VEGF in the pathogenesis of CNV is well understood and known.Ranibizumab is a fragment of a humanized monoelonal antibody.It plays an inhibitory role on CNV and reduces vascular permeability by binding to VEGF.Some clinical trials and literature have proved the clinical efficacy of ranibizumab via intravitreal injection for wet AMD.But the systematized administration is vital important.Some high-quality researches showed that the optimal timing for ranibizumab treating wet AMD is the first 3 months.It is recommended that ranibizumab is intravitreally injected monthly in the initiation for least 3 months.Sequent managing regimen should be made depended on the image of optical coherence topography and fundus fluorescine angiography and visual acuity change.Individuated strategy or combined method with PDT are beneficial to the active lesion in the consecutive treatment of ranibizumab for CNV and may be a good choice in order to decrease the injection times.This article systematically searched and summarized the relative literature and offered the high-quality evidences for the clinical evaluation of ranibizumab treating wet AMD.

This study was aimed to explore the tic disorders ( TD ) pathogenesis induced by chronic rhinitis . A total of 400 TD children were all investigated with self-made TD general questionnaire , Yale Global Tic Severity Scale ( YGTSS ) , TD diagnostic criteria by DSM-IV , chronic rhinitis diagnostic criteria by Zhu-Fu-Tang Textbook of Pediatrics ( 7th edition ) . A total of 258 TD children accompanied with chronic rhinitis were screened . Levels of peripheral blood T lymphocyte subsets , immunoglobulins , IL-6 , TNF-α and other related immunological indexes were detected to analyze pathogenesis. The results showed that the nose movement symptom scores of TD children with rhinitis were higher than the score of TD children without rhinitis . The score of arm-lifting and hand movement in TD children with rhinitis was lower than the score in the TD children without rhinitis . And the YGTSS score of TD children with rhinitis was higher than TD children without rhinitis. Compared with TD children without rhinitis, the level of peripheral blood T lymphocyte subsets in TD children with rhinitis was obviously reduced ( P < 0 . 05 ) . Compared with TD children without rhinitis , the level of immunoglobulins in TD children with rhinitis was slightly reduced . The level of IL-6 in TD children with rhinitis tended to increase , but the level of TNF-α tended to reduce . It was concluded that chronic rhinitis is one of the factors to induce TD .

Objective To investigate the relationship between serum uric acid level and the severity of pregnancy induced hypertension (PIH) in pregnant women with hypertension.MethodsFrom January 2016 to July 2016 in our hospital for treatment of 100 cases of patients with pregnancy induced hypertension, as PIH group, divided into severe hypertension group (33 cases), moderate PIH group (33 cases) and mild PIH group (34 cases), then select 100 cases of normal pregnant women normal pregnancy group.The serum uric acid levels were measured and the results were analyzed.ResultsThe average serum uric acid levels in PIH group (359.75+89.23) mol/L was significantly higher than the control group (188.24+54.54) mol/L in PIH group, the average level of serum uric acid in severe, moderate and mild degree is decreasing, respectively (449.32+81.11), (352.89+76.41), (288.76+59.58) mol/L, a statistically significant difference between the groups (P<0.05).ConclusionThe detection of serum uric acid level in pregnant women can effectively determine the degree of pregnancy induced hypertension in pregnant.

Adult ; Aged ; Carcinoma ; Humans ; Middle Aged ; Nasopharyngeal Neoplasms ; diagnosis ; pathology ; therapy ; Neoplasm Recurrence, Local ; therapy ; Salvage Therapy ; Young Adult

Objective To study the radiosensitization of histone deaeetylases inhibitor(HDACI) panobinistat in prostate cancer cells in vitro,as well as the possible mechanisms.Methods IC20 of two prostate cancer cell lines(LNCaP and PC-3)was determined using MTI assay.Cells received a single dose irradiation of 0,2,4,6,or 8 Gy using 6 MV X-ray for radiosensitivity experiment,but only 2 Gy for western blot and flow cytometry.Radiosensitization of panobinostat was investigated with clonogenic assay,and sensitizing enhancement ratio(SER)was calculated with single-hit multi-target model.Western blot was used to compare γH2AX expression.Flowcytomctry was used to detect the cell cycle distribution.Results IC20 of LNCaP and PC-3 was 2.5 and 10.0 μmol/L,respectively.SER of panobinostat at IC20 was 1.37(D0 ratio)and 1.11(Dq ratio)for LNCaP cells,and 1.78(D0 ratio)and 1.17(Dq ratio)for PC-3 cells.Expression of γH2AX gradually decreased in the 2 Gy irradiation-alone cells standing for the DSB repair,while γH2AX expression was persistent in the combination group.Irradiation triggered a G2/M arrest 6-12 hours after irradiation in LNCaP and PC-3 cells.G2/M arrest was observed when cells were treated with panobinostat for 24 hours,however,no significant change concerning cell cycle distribution was showed when cells received further irradiation.Conclusions Panobinostat Call radiosensitize prostate cancer cells,which may be related with increased DNA DSB,inhibition of DSB repair and attenuation of cell cycle modulation after irradiation.

Objective To investigate the similarities and differences in research hot pots between International Journal of Pediatrics and Pediatrics in the last five years.Methods The key words with high frequent occurrence were extracted from Wanfang database,and the Mesh with high frequent occurrence were enrolled in this study.Results The similarities of the hot pots between the two journals were asthma,neonatal diseases and neoplasm.The differences lied in that the hot pots of International Journal of Pediatrics included congenital heart diseases,allergic diseases and renal diseases,whereas the hot pots of Pediatrics were obesity,child health care and attention deficit disorder with hyperactivity.Conclusion By employing co-words cluster analysis,the research focuses of the two journals are found out,and its differences are compared.

Objective To investigate the relationship between childhood obesity,asthma and lung function.Method Based on body weight and whether with asthma the 45 children were divided into normal control group,asthma group,obesity and asthma group,15 cases each.Each child was measured leptin levels and pulmonary function was measured with a spirometer.Each asthmatic children was surveyed by Asthma Control Test (C-ACT) questions.Results Asthmatic children have varying degrees harm of lung function.Obesity and asthma group compared with asthma group of children is more decreased in lung function.Children with asthma compared with normal control group of children is more higher in serum leptin concentration.Obesity and asthma children is highest in serum leptin concentrations.Asthmatic children C-ACT average score than obesity and asthma score,the difference was statistically significant (P ＜ 0.01).Conclusion Lung function and leptin abnormalities are exist in children with asthma.Also in the case of asthma,the abnormal levels of lung function and leptin in obese children compared with normal-weight children are more apparent,C-ACT score are more lower,and asthma is more severe.Weight control in obese children with asthma is favorable measures to control asthma attacks.