Objective:To establish a method for the uncertainty evaluation of the determination of benzoic acid. Methods: The content of benzoic acid was determined by acid-base titration. By constructed mathematics model, the source of the measurement uncer-tainty was analyzed, and the uncertainty components were quantized and combined. Results:The expanded uncertainty of benzoic acid was 0. 36% and the results were expressed as(99. 99 ± 0. 36%,k=2). Conclusion:The mathematics model is reasonable and relia-ble,and can be used in the uncertainty evaluation of content measurement of benzoic acid.

AIM:To analyze the clinical significance of axial length, diopter and retinal nerve fiber layer thickness in hyperopia children with anisometropia amblyopia. ·METHODS: From January 2015 to January 2017 in our hospital for treatment, 103 cases, all unilateral, were diagnosed as hyperopia anisometropia amblyopia. The eyes with amblyopia were as experimental group ( 103 eyes), another normal eye as control group (103 eyes). We took the detection with axial length, refraction, foveal thickness, corrected visual acuity, diopter and the average thickness of retinal nerve fiber layer. ·RESULTS: Differences in axial length and diopter and corrected visual acuity were statistically significant between the two groups (P<0. 05). The two groups had no significant difference in retinal nerve fiber layer thickness (P>0. 05). There was statistical significance difference on the foveal thickness (P<0. 05). There was a negative correction correlation between the visual acuity, axial length with foveal and peripapillary nerve fiber layer thickness, but no significant difference in correlation ( P>0. 05 ). The positive correlation between diopter with nerve fiber layer thickness of foveal and around the optic disc were no statistically significant difference (P>0. 05). ·CONCLUSION:Retinal thickness of the fovea in the eye with hyperopic anisometropia amblyopia were thicker than those in normal eyes;the nerve fiber layer of around the optic disc was not significantly different between the amblyopic eyes and contralateral eyes. The refraction and axial length had no significant correlation with optic nerve fiber layer and macular foveal thickness.

Objective To explore the effect of seminar applied in instruction of Introduction of Nursing Science for advanced vocational nursing students.Methods A total of 246 advanced vocational nursing students admitted in 2013 from class 1 to class 5 were named as the control group,who received traditional teaching method.Meanwhile a total of 252 advanced vocational nursing students admitted in 2013 from class 6 to 10 were set as the experimental group,and were given seminar teaching.We adopted California Critical Thinking Dispositions Inventory Scale,Academic Self-efficacy Scale and an anonymous questionnaire for teaching satisfaction.Theoretical examination was used to be final evaluation.Results The scores of theoretical examination in the experimental group were higher than that of the control group.The scores of critical thinking ability and academic self-efficacy of the experimental group were higher than those of the control group,with a significant difference.The result of the anonymous questionnaire showed that the experimental group showed a higher satisfaction degree with seminar teaching method.Conclusions Seminar teaching method applied in Introduction of Nursing Science is feasible and effective,which is better than traditional method in improving critical thinking ability,academic self-efficacy and teaching satisfaction.

Accidents, Occupational ; Adult ; Female ; Humans ; Male ; Phosgene ; analogs & derivatives ; poisoning

The author had carried out the analysis on the sports value of competitive sports for the disabled from the bioethical view , based on the following points:the individual value in the disabled competitive sports;core val-ue of competitive sports for the disabled ethics;the meaning of life and the value of competitive sports for the disa-bled;humanistic rational values of the instrumental rationality;competitive sports for the disabled and links to the real life, and so forth.

ObjectiveTo observe the effect of toosendanin on proliferation and apoptosis of human hepatoma cell line HepG2 cells,and to study the mechanism.MethodsHuman hepatocellular carcinoma HepG2 cells were randomly divided into control group(group A) does not add any medication,the observation group(group B) joined TSN 80mmol/ml,cultured cells for 24,48,72 hours.HepG2 cells proliferation inhibition rate was measured by MTT; Hepatocellular carcinoma HepG2 cell cycle and apoptosis rate was deteded by flow cytometry.ResultsTECAN determination revealed two groups of hepatocellular carcinoma cell proliferation had statictical significance ( x2 =5.33,P ＜0.05 ) ;flow cytometry revealed two groups S,G0/G1,M/G2 phase of the cell rate ( t =6.31,6.26,6.56,all P ＜0.05 ),apoptosis rate had significant difference ( x2 =6.15,P ＜ 0.05 ).ConclusionToosendanin could obviously inhibit the proliferation of HepG2 cells,and induce apoptosis of tumor cells.

Objective To investigate the impact of the different mode of delivery on postpartum lactation.Methods 1 678 pregnant women in our hospital were divided into cesarean section group(798 cases) and natural delivery group(880 cases).Open milk after delivery,breast fullness,the number of neonatal sucking and post-natal self-assessment milk water were compared between the two groups of pregnant women,and the impact of the mode of delivery on postpartum lactation was analyzed.Results Whether or not began to lactation (721/77cases),and the time of labor to beginning to lactation [(33.45 ± 9.68) h],and whether or not having breast fullness feeling (512/286 cases),and the time of labor to breast fullness feeling[(57.12 ± 13.90) h] in cesarean section group had significant difference with those of spontaneus labor group [(845/35 cases),(30.12 ± 10.25) h,(690/190 cases),(52.98 ±15.09) h] (x2 =21.613,t =2.452,x2 =41.813,t =2.818,all P ＜ 0.05).There was no significant difference in selfrating milk score [(2.02 ± 0.45) points vs (2.45 ± 0.51) points],and newborn sucking times 4 ～ 6d postpartum [(5.21 ±1.21) times vs (5.89± 1.82)times](t =2.012,2.532,all P ＜0.05).Conclusion Efficiency of the breast-feeding was affected by the impact of the mode of delivery.Rationalized breastfeeding guidance was necessary to strengthen for pregnant women by cesarean section.

Dextrocardia is formed by the disruption of the normal left-right (L-R) asymmetry of the thoracoabdominal organs.Asymmetric expression of Nodal,Pitx2,Cited2 have affected L-R asymmetry of vertebrate significantly,and generated dextrocardia,mesocardia,ectopia cordis,heterotaxy and so on.Dextrocardia is often accompanied by congenital cardiac malformation.Therefore,it is so difficult to be treated that it has poor prognosis.This paper reveiws the molecular mechanism and it's regulation of dextrocardia for proceeding the genetic diagnosis and early intervention of dextrocardia sequentially in order to improve the survival rate and life quality of patients.

Objective To evaluate the clinical effects and safety when adopt smoothing liver and invigorating spleen treatment to cure diarrhea-predominant irritable bowel syndrome (D-IBS) characterized by liver stagnation with spleen deficiency.Methods 71 patients met the inclusion criteria of D-IBS were randomly divided into the treatment group(36 cases)and control group(35 cases),the treatment group used Shugan Jianpi therapy,traditional Chinese medicine Tongxieyao Fang and additive Shenlingbaishu Decoction; the control group taking Lactobacillus Complex Capsules treatment.Two groups of the time of taking medicine for 4 weeks.Observation and analysis of comprehensive effects and D-IBS symptom score before and after treatment.Results There was no statistical significance in the two groups before treatment,symptom total score difference,after the treatment group and the control group of symptom score were (4.83 ± 3.30),(7.00 ± 3.96),with a significant difference between the two groups (t =2.51,P ＜ 0.05) ; the total effective rate of treatment group was 91.67％,which was higher than 68.57 ％ in the control group(x2 =5.98,P ＜ 0.05).Conclusion The adoption of the smoothing liver and invigorating spleen treatment characterized by the use of Tong Xie Yao Fang and additive ShenLingBaiZhu decoction to cure D-IBS patients with liver stagnation and spleen deficiency has a relatively satisfactory clinical curative effect.

The influence of refined konjac meal(RKM) on the absorption of dietary zinc, iron, and calcium in rats and human subjects was studied. RKM containing 75-80% glucomannan was prepared and refined from tubers of Amor-phophallus konjac K. KochMale and female SD rats aged 6 weeks were equally divided by randomized blocking into 5 groups of 10 (5M, 5F). The control group was fed on a basai diet, and the other four test groups were also fed on a basal diet but substituting 0.5%, 1%, 5%, or 10% of the RKM respectively for corn-starch. The feeding experiment lasted six weeks during which balance studies of the tested minerals were carried out at the end of every two weeks. At the end of the second week the absorption of zinc was significantly lower in the 5% and 10% RKM groups than that of the control group, but from the week on, the digestibility lowering effect of RKM disappered, perhaps on account of adaptation. As for iron or calcium, no digestibility lowering effect of RKM was observed during the whole experimental period. No reduction in serum levels of these three minerals was observed.Twelve young adult subjects (6M, 6F) participated in a 9-day feeding trial. For the first three days (control period), they were taken ordinary meals without RKM, but during the subsequent 6 days (test period), RKM was added to the ordinary meals in 3-gram increments every two days (i.e beginning with 3g per day for two days, then 6g per day for the consecutive two days and finally 9g per day for the last two days). Mineral analyses were conducted on the subjects' food and stool samples which were collected and weighed separately on the third and ninth day. Results obtaiud indicated that consuming a therapeutic dosage of RKM in association with meals did not influence the absorption of dietary zinc, iron or calcium.