DOTA and its derivatives are most widely uscd novel bifunctional chelators.They have not only mature synthesis route,but also good coordination and chelating ability.Therefore,DOTA metal complexes of DOTA-peptide conjugates are increasingly used as MRI contrast agents,radionuclide targeted imaging agents and therapeutic radiopharmaceuticals in the biomedical field.This review covers the bifunctional derivatives of DOTA,the synthesis of DOTA-peptide conjugates,and the applications of DOTA-peptide conjugate metal complexes.

Objective To investigate the influence of carbon monoxide(CO) on the content of atrial natriuretic peptide(ANP) in ventricular tissues of rats.Methods The left and right ventricular tissues of 12 Wistar rats were divided into control group(n=12) and hemin group(n=12,the final concentration of hemin was 10~(-4) mol/L),respectively.The ventricular tissues were successly incubated in(37 ℃) thermostatic waterbath with Krebs solution as supernatant for 4 hours.After incubation of the ventricular tissues,radio-immunity method was applied to determine the content of ANP in the supernatant.Results Compared with the content of ANP in the supernatant in control group,it was significantly decreased both in left [(9.72?3.59) vs(52.05?31.65) ng/(g?wet tissue),P

Objective An inductively coupled plasma mass spectrometry (ICP-MS) method was established for the determination of inorganic elements in stems and leaves of Scutellaria baicalensis. The inorganic elements in the extracts were analyzed and evaluated. Methods ICP-MS was applied to determine 23 kinds of inorganic elements in samples digested by microwave in stems and leaves of S. baicalensis from eight regions, and the results were analyzed by principal component analysis (PCA) and correlation analysis. Results There were no differences in the types of inorganic elements in stems and leaves of S. baicalensis from different regions, but the content of them varied greatly. Among them, Fe, Zn, Cu, Mn, Cr, Co, Ni, Sr, B, and Ni were essential elements of the human body. The content of Al and Fe was the highest, and the content of B, Ti, Mn, Sr, and Ba was higher than others. The total content of heavy metals and harmful elements of samples were up to the mustard, except S10. The contents of Cd in S2, S10 and S22-S30 exceeded standards. Thirty-five batches stems and leaves of S. baicalensis were divided into eight groups, which were analyzed by thePCA with the contents of 23 elements as variates. These regions which have different geographies and climates might lead to the differences in the contents of inorganic elements in the samples. Four factors (F1-F4) were selected to make a comprehensive evaluation. The evaluation function was F = 0.444 2 F1 + 0.166 6 F2 + 0.129 1 F3 + 0.056 4 F4. The results showed that the scores of samples from Shanxi and Shaanxi were higher, which indicated that the qualities of above samples were better than those of other samples. Conclusion In this study, an accurate and efficient method for the analysis and determination of inorganic elements in stems and leaves of S. baicalensis from different regions was established, which provided a scientific reference for the quality control, safety evaluation, and comprehensive utilization of S. baicalensis resource.

Authors reviewed the present study situation of acupuncture-moxibustion theropy on osteoarthritis, and the possible principle according to the universal one have known of acupuncture and its function on other aspects especially on arthritis, so as to explore direction for further research work of clinical and principle study.

Humans ; MicroRNAs ; Occupational Medicine

Objective To analyze the clinical and immunological features of 45 pediatric patients with lupus nephritis (LN). Methods Forty-five LN patients were included in this study. Clinical, pathological data and immunological parameters were retrospectively analyzed. Results Forty-five LN patients had 6 males and 39 females, with the mean onset age of (10.9 ± 2.8) years. Acute nephritis was the most common type, accounting for 42.2%. Nephrotic syndrome accounted for 31.1%. Renal biopsy showed class II (17.8%), III (4.4%), IV (48.9%), V (2.2%), V+III (6.7%)and V+IV (13.3%)in 42 cases. The remis-sion rate reached 91.1%in the early therapeutic stage, and 15.0%patients recurred after 24-month follow-up. Conclusions The clinical manifestations of LN children are diverse. The renal pathology is complex. The clinical manifestations in part of the chil-dren are not consistent with renal pathology.

Objective; To evaluate the effects of gingival shade guide in visual shade-matching in vitro models. Methods; 42 tabs of Shofu NCC shade guide were adopted for color measuring. Each tab was fixed in the middle of Gingiva-Indicator, Guray(1,2,3,4) respectively and located in a black box. The measurements were made using the Shadepilot instrument. The color parameters ( CIE1976L * a * b * ) of cervical, body, incisal area of each tab were recorded. The color difference A E value in the same position of each tab was calculated between non-gingival shade guide group and Gumyl ,2,3,4 groups with Shadepilot software. Results; The AE value in the same position of 42 tabs between non-gingival shade guide group and 4 gingival shade guide groups were; cervical (2.93±0.79)NBS,body(0.51±0.27)NBS and incisal(0.61 ±0.29)NBS. In the cervical, body and incisal area, there was no statistically difference among Gumyl ,2,3,4 groups on affecting shade of tabs(P >0. 05). Conclusion; The gingival color has an effect on the cervical shade of tabs, and it can be recognized by human eyes, while gingival color has little effect on the shade of body and incisal area.

Angiogenesis Inhibitors ; therapeutic use ; Antineoplastic Combined Chemotherapy Protocols ; therapeutic use ; Carcinoma, Hepatocellular ; blood supply ; metabolism ; pathology ; therapy ; Chemoembolization, Therapeutic ; methods ; Endostatins ; therapeutic use ; Humans ; Liver Neoplasms ; blood supply ; metabolism ; pathology ; therapy ; Microvessels ; pathology ; Neovascularization, Pathologic ; pathology ; Vascular Endothelial Growth Factors ; metabolism

Objective This study aimed to assess the effects of active and passive smoking on chronic kidney disease (CKD) in patients with type 2 diabetes mellitus (T2DM).Methods Seven hundred and five patients with T2DM were recruited in the study and were divided into three groups based on smoking status as active smokers,passive smokers and non-smokers.Twenty-four hour urinary albumin excretion (24hUAE) was measured,and estimate glomerular filtration rate (eGFR) was calculated with age and blood creatinine levels.Results (1) The proportion of CKD in T2DM in the present study was 31.63％ (223/705) with 28.6％ (22/77),30.0％ (15/50) and 29.6％ (73/247) for non-smokers,passive smokers and active smokers in men,and 29.9％ (40/134),35.9％ (66/184) and 7/13 for non-smokers,passive smokers and active smokers in women,respectively.In comparison with non-smokers,a higher risk of CKD was found in both passive and active smokers (OR =1.07 and OR =1.05 in men ; OR =1.31 and OR =2.74 in women,respectively).(2) Compared with non-smokers,passive smokers had a significant higher risk for albuminuria in women (OR =2.02,P =0.016).(3) After adjusting for gender,age,duration of T2DM,BMI,systolic blood pressure,glycosylated hemoglobin A1C and lipids,there was a significant decrease in eGFR between active and never smokers (P =0.018)or passive smokers (P =0.000) in women.No differences could be found in eGFR between each smoking statues in men.Conclusions Smoking exposure alone confers a high risk for CKD in patients with T2DM.Our results highlight an importance in implementation of a smoke-free environment for patients with T2DM.

BACKGROUND:Orthotopic lung transplantation model in a rat is the key to investigate the chronic rejection after lung transplantation. However, the precise surgical technique and difficult operation limit the application of the model. OBJECTIVE:To improve the process of anesthesia and lung transplantation, and to establish a rapid, safe and reversible rat lung transplantation model. METHODS:A total of 42 rats were used to establish the model, including 21 donor models and 21 receptor models. The donor lung was excised by median sternotomy with dissection of the left lung and implantation of cuffs (intravenous catheters cut into 1.5 mm sections). The left lung was implanted in the recipient by lateral thoracotomy using the cuffs for anastomoses. The duration of surgery and success rate of transplantation were recorded and calculated. RESULTS AND CONCLUSION:The survival rate of rats after lung transplantation was 100%. The time of left donor lung extraction was (35.3±5.1) minutes in average. The time of placing cuff in donor lung was (12.5±4.6) minutes in average. The surgical procedure time of recipient was (50.2±3.3) minutes. The time of arteriovenous and bronchus casing anastomosis was (27.7±6.2) minutes. After pulmonary artery and vein blood flow was disparked, the whole lung turned red rapidly, blood perfusion was sufficient, venous returned unimpeded;after mechanical ventilation resumed, al graft lungs expanded wel . This improved anesthesia and lung transplantation technique in rats can provide a valid, reliable and reproducible animal model for studying immune responses and rejection in lung transplantation.