Objective To explore the clinical manifestation,physiological index changes and consequences of food poisoning caused by Salmonella weltevreden(S.weltevreden).Methods Bacteria culture and isolation were proceeded with stool specimens from patients who got food poisoning in a university in Chengdu on May 25,2016.Blood routine examination,coagulation function test,C-reactive protein(CRP) and D-Dimer tests were also proceeded with whole blood or plasma samples from food poisoning patients.Results A total of 69 patients were identified food poisoning caused by S.weltevreden,and S.weltevreden was isolated from all of their stools specimens.The common clinical manifestation of these patients were high fever,stomachache and diarrhea with watery stool.91.3% of patients had a blood routine white blood cells(WBC) increasing in different degrees,20.3% of patients had a WBC increasing in stool specimens.The CRP level of all patients were abnormally increased,and the procalcitonin(PCT) level,D-Dimer level and coagulation function were also abnormal.Conclusion The clinical symptoms of food poisoning caused by S.weltevreden are seriously,even physiological function of some patients are changed tremendously or life-threateningly.We should pay attention to the dangers of S.weltevreden to human life in addition to strengthen food safety.

Objective · To clarify effect of adipose stem cells derived conditioned medium (ASCs-CM) on fibrogenesis of dermal fibroblasts costimulated by transforming growthfactor-β1 (TGF-β1), and explore the possible paracrine pathway of ASCs in regulating the dermal tissue rehabilitation.Methods · Co-stimulated by TGF-β1, dermal fibroblasts were treated with different concentrations of ASCs-CM. Variouscellular events including cell proliferation, apoptosis, and mRNA and (or) protein levels of α-smooth muscle actin (α-SMA), type Ⅰ collagen (COL-1) , type Ⅲ collagen (COL-3), hepatocyte growth factor (HGF), basic fibroblast growth factor (FGF-2) were explored. Furthermore, the effect of HGF antibody on apoptosis of fibroblasts caused by ASCs-CM and TGF-β1 were also observed. Results · ASCs-CM inhibited fibroblasts proliferation caused by TGF-β1 and lead to cell apoptosis. α-SMA expression in fibroblasts was attenuated by 10% ASCs-CM +TGF-β1. It was demonstrated that 100% ASCs-CM with TGF-β1 had promoted collagens (especially COL-3) expression in fibroblasts, and increased HGF mRNA level as well. HGF antibody inhibited fibroblasts apoptosis produced by 100% ASCs-CM and TGF-β1. Conclusion · ASCs may have an effect on fibrogenesis of dermal fibroblasts co-stimulated by TGF-β1 through a paracrine way.High concentration of ASCs-CM not only increases collagen production and secretion, but also inhibits fibroblasts proliferation and accelerates apoptosis.

Objective: To help medical device registration applicant understand the content of clinical evaluation, guided on the clinical evaluation method, and also putted forward several suggestions of the improvement scheme for our country registered clinical assessment and review of the work. Methods:Described the Global Harmonization Task Force (GHTF) medical device registration guidance for clinical evaluation, combined with the status quo of supervision in China, and made some specific scheme on China's medical device of clinical evaluation work. Results: Introduced the contents of the clinical evaluation and the relationships between the factors, in order to help the medical device registration applicant and Chinese regulators to do clinical evaluation work of scientific and reasonable. Conclusion:The definition of medical device registration clinical evaluation should clear the clinical evaluation, and to develop guidance for clinical evaluation of the corresponding.

Objective: To observe the application values of 64-slice spiral CT angiography (CTA) in diagnosing persistent trigeminal artery in an effort to improve the understanding of this vascular variation. Methods: The imaging data of 5 patients with persistent trigeminal artery in 2734 patients who performed 64-slice CTA of craniocervical arteries were analyzed retrospectively. The incidence, origin, running, and blood supply from posterior circulation of persistent trigeminal artery, and its complicating other vascular malformations were analyzed. Results: Circled digit oneThe incidence of persistent trigeminal artery was 0.18% (5/2734). Three of them were on the right side and 2 were on the left side. Circled digit twoAll the trigeminal arteries originated from the cavernous segment of internal carotid artery. Four patients were lateral type running and one (20%) was internal type running. According to Saltzman's classification about the blood supply in posterior circulation: one patient was Saltzman type I , one was type II, and three were type III. Circled digit threeThree patients had basilar artery and bilateral vertebral artery hypoplasia at the point of proximal anastomosis, one had complete atrophy of basilar artery at the point of proximal anastomosis, one complicated with aneurysm of the persistent trigeminal artery, and one complicated with fenestrated middle cerebral artery on the contralateral side. Conclusions: 64-slice CTA can clearly, rapidly, noninvasively, and accurately display the persistent trigeminal artery and its running. Understanding of this abnormal vessel in the sellar or suprasellar regions before surgery and interventional treatment will help develop a reasonable surgical and interventional regimen to avoid the risks caused by improper management.

Based on the extensive experience in circuit serving for military medical equipment,problems in managing military medical equipment are discussed and solutions are proposed so as to strengthen management standardization of medical equipment in basic unit army and enhance the support ability of medical equipment.

OBJECTIVE:To study the effects and mechanism of rapamycin on invasion and metastasis of cervical cancer HeLa cell. METHODS:HeLa cells were divided into control group and rapamycin low-dose,medium-dose and high-dose groups (10, 30,100 nmol/L). After treated for 48 h,cell viability was measured by MTT assay,and inhibitory rate was calculated;migration and invasion of cell was tested by Transwell assay. The expression of matrix metalloproteinase 2(MMP-2),MMP-9,Vimentin and E-cadherin,and phosphorylation of protein kinase B (Akt),mammalian target of rapamycin (mTOR) were detected by Western blot. RESULTS:Compared with control group,the inhibition rate of cell viability was increased in rapamycin groups(P<0.01);the number of invasion and metastasis cells decreased(P<0.01);the expression of MMP-2,MMP-9 and Vimentin were decreased (P<0.01 or P<0.05);the expression of E-cadherin was enhanced(P<0.01 or P<0.05);the phosphorylation of Akt and mTOR were reduced (P<0.01). CONCLUSIONS:Rapamycin could inhibit invasion and metastasis of HeLa cell via Akt/mTOR signal pathway.

Objective:To analyze the clinical materials of the patient with Van Wyk and Grumbach syndrome (VWGS),and to explore the pathogenesis,clinical characteristics,diagnosis,differential diagnosis and treatment method.Methods:The clinical data of a patient with VWGS was analyzed retrospectively.Results:The patient presented autoimmune thyroiditis, sever long-standing hypothyroidism, bilateral ovarian cysts, hyperprolactinaemia and a secondary pituitary adenoma. After thyroxine replacement treatment, the thyroid function and prolactin (PRL)level recovered to the normal levels and the ovarian cyst was shrunk within 1 month. Conclusion:The clinical manifestation of VWGS is special.And it’s easy to misdiagnose this syndrome as pituitary adenoma or ovary cyst and then operation is performed.Thyroid hormone replacement can completely resolve the symptoms and hormone abnormalities.Grasping the clinical characteristics of this syndrome and making correct diagnosis is critical to avoid unnecessary operation.

Aim To investigate the effects of breviscapine(Bre)on Na+,K+-ATPase activity of proximal tubule(PT) and its renal protection in early diabetic rats. Methods The rats were divided into three groups: diabetic model (DM group), Bre treatment (Bre group) and normal control (NC group). Rats were administrated with Bre(20 mg?kg-1?d-1, ip) in Bre group, with normal saline(ip) in DM and NC groups for four weeks after diabetes induction with streptozotocin (STZ 65 mg?kg-1, ip) in Bre and DM groups. The urine and blood samples were collected from two intra-ureteral cannulas and the heart, respectively, under anesthesia four weeks after diabetes induction. After one of the renal arteries being perfused, the renal cortex was incubated and PT segments were microdissected freehand under microscope before the Na+,K+-ATPase activity in the segments were assessed by liquid scintillation counter. The blood glucose, levels of creatinine in serum and in urine were assayed. The microalbumin , ?2-microglobulin(?2-MG) of urine and endogenous digitalis-like substance (EDLS) of serum were measured respectively by radioimmunoassay. Results The PT Na+,K+-ATPase activity in NC group was(959.11?117.35) pmolPi?mm-1?h-1, and that in DM group was significant higher for (1893.53?383.90) pmolPi?mm-1?h-1 than it(P

Objective To investigate the effect of therapy on the patients of gastric ulcer by antrectomy ulcer locally excision with anti-helicobacter pylori drugs.Methods According to the principal of vertical incision and parallel suture,the ulcer was locally existed by parallel axis.The area beyond the margin of ulcer one centimeter must be resected,26 cases have been performed the antrectomy retaining pylorus and gastroduoden-cstomy.After operating all of the patients had taken anti-helicobacter pylori drugs for 21 days.Results We follow up this group of patients for 5 years.Symptom that occurred preoparation disappeared in 24 cases and patients' weight increased.2 patients performed preservative therapy of posthepatitis cirrhosis.After 5 years all of them had been taken gastr ofiberscope reexamination and the result of no recurrence of ulcer and HP negative.Conclusions This method is a simple and safe procedure with quite small injury.It prevents recurrence and is fit for the physiology of human body with few postoperative complication and retain pylorus.

This work summarizes the research development and molecular mechanism of Ebp1, a member of the proliferation-associated 2G4 family, in tumor proliferation and invasion. This research serves as a basis and support for further research on the mechanism of tumor proliferation and invasion. The low expression of Ebp1 in various cancers promotes tumor proliferation and invasion. Ebp1 inhibits E2F1, cyclin D1, and cyclin E transcription by interacting with Rb, human histone deacetylase 2, and the transcriptional repressor Sin3A. This inhibition triggers cell cycle arrest and suppresses cell proliferation. Ebp1 also influences cancer invasion and migration. However, the underlying mechanisms remain unknown and require further exploration.