Objective To study the effect of hyperbaric oxygen (HBO) therapy on changes in the prolifera-tion of endogenous neural stem ceils (NSCs) in neonatal rats after hypoxie ischemic brain damage (HIBD). Meth-ods A total of 150 Sprague-Dawley rats aged 7 d were randomly divided into a normal eontrol group ( CON ), a HIBD group and a HBO treatment group. HBO was administered to the HBO treatment group within 3 h after HIBD at 2 atmospheres, once daily for 7 d. The HIBD model rats were subjected to unilateral carotid artery ligation followed by 2 h of hypoxia (8% O2> ). BrdU/nestin immunofluorescence was used to examine the proliferation of NSCs dynami-cally in the subventricular zone (SVZ) and the dentate gyms (DG) at the 3rd h, 21st h, 3rd d, 7th d and 14th d af-ter HBO therapy. Nestin protein was detected by Western blot analysis at various time points after HIBD. Results In the HIBD rats treated with HBO, proliferation of endogenous NSCs was observed in the SVZ and DG. The prolifer-ating NSCs increased at the 3rd h and 21st h after HBO therapy in the SVZ and DG respectively, peaked at the 7th d after HBO therapy, and decreased by the 14th d after HBO therapy, though their level was still higher than that in the controls. The Western blot analysis showed that nestin protein began to increase at the 21st h after HBO therapy, peaked at the 7th d after HBO therapy, then decreased. Conclusion HBO administered within 3 h after HIBD can promote proliferation of endogenous NSCs in neonatal rats after HIBD.

Multi-disciplinary team(MDT)mode is regarded as the key to standardized diagnosis and treatment of malignant tumors. The model, however, encounters such roadblocks in the current form of MDT organization, as costly clinical resources and time consumption, low efficiency, poor management of participating experts in MDT, and lack of enforceability of the therapeutic decisions made. This paper summarized the practical MDT experiences of the Affiliated Cancer Hospital of Shandong First Medical University. It introduced the construction of an intranet-based MDT system covering a large proportion of newly diagnosed malignant tumor patients, and the practices and achievements of such MDT management system under hospital administrative guidance. The authors proposed to use reporting ratio as the main assessment indicator in promoting MDT, and that to define the performance, responsibilities and rights in MDT practice. These measures aim at to upgrading individual behaviors of doctors to organizational behaviors of hospitals, and providing cancer patients with more standardized, comprehensive and personalized diagnosis and treatment decisions.

To investigate the formation of polystyrene nanoplastic-plant protein corona and its potential impact on plants, three differently modified polystyrene nanoplastics with an average particle size of 200 nm were taken to interact with the leaf proteins of Impatiens hawkeri for 2 h, 4 h, 8 h, 16 h, 24 h, and 36 h, respectively. The morphological changes were observed by scanning electron microscopy (SEM), the surface roughness was determined by atomic force microscopy (AFM), the hydrated particle size and zeta potential were determined by nanoparticle size and zeta potential analyzer, and the protein composition of the protein corona was identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The proteins were classified in terms of biological processes, cellular components, and molecular functions to study the adsorption selection of nanoplastics to proteins, investigate the formation and characteristics of polystyrene nanoplastic-plant protein corona and predict the potential impact of protein corona on plants. The results showed that the morphological changes of the nanoplastics became clearer as the reaction time extends, as evidenced by the increase in size and roughness and the enhancement of stability, thus demonstrating the formation of protein corona. In addition, the transformation rate from soft to hard protein corona was basically the same for the three polystyrene nanoplastics in the formation of protein corona with leaf proteins under the same protein concentration conditions. Moreover, in the reaction with leaf proteins, the selective adsorption of the three nanoplastics to proteins with different isoelectric points and molecular weights differed, and the particle size and stability of the final formed protein corona also differed. Since a large portion of the protein fraction in protein corona is involved in photosynthesis, it is hypothesized that the formation of the protein corona may affect photosynthesis in I. hawkeri.
Polystyrenes/chemistry* ; Protein Corona/chemistry* ; Microplastics ; Plant Proteins ; Chromatography, Liquid ; Tandem Mass Spectrometry ; Nanoparticles/chemistry*