Objective To compare the yield and speed of detection of Salmonella subsp, enterica serotypo Paratyphi A from the blood of patients with suspected paratyphoid fever A. Methods With the BacT/ALERT 3D system and paired aerobic and anaerobic bottles (AEB, ANB) that were each filled with 5 ml of blood, the blood culture of 13 500 suspected paratyphoid fever A patients were performed. Results A total of 4 060 isolates were detected. About 3 149 were detected from both AEB and ANB. Four hundred and sixty-one isolates were detected only from the AEB and 450 were only from the ANB. The detection rates of the AEB and ANB were all 26.7% (χ<'2>=0.023, P=0.880). The increased detection rate attributed to the additional blood volume in the AEB and ANB were 11.3% and 11.1%, respectively. The detection speed differed between the two medium formulations. The time to detection was (23.66±15.89) h and (25.48±16.92) h for3 149 isolates, respectively (t=7.007, P<0.01).The mean time to detection was 31.80±20. 97 for 461 isolates discovered with AEB and (33.45±20.72) h for 450 isolates discovered with ANB. Conclusion The blood volume is an important factor in determining the detection rate of blood culture. Although no statistical difference for positive rate was found between the AEB and ANB, more isolates was detected earlier in AEB.

BACKGROUND:Sodium hyaluronate is a polysaccharide polymer biomaterial, which is considered to have a certain effect to repair the cartilage surface, reduce the release of inflammatory mediators, and promote meniscal repair. OBJECTIVE:To observe the therapeutic effect of Fast-Fix combined with sodium hyaluronate in meniscal repair under arthroscope. METHODS:Eighty-six patients with meniscus injury admitted at the Department of Orthopedics, CNPC Central Hospital, from March 2008 to March 2014 were enrol ed in the study. The average age was 25.5 years, and the average duration of disease was 1.5 months. The main clinical manifestations included knee joint pain and swel ing after exercise and positive McMurray signs. The MRI results showed meniscus tear or degeneration. Arthroscopic repairing surgeries were performed with Fast-Fix combined with sodium hyaluronate injection (2.5 mL at the end of surgery), and then sodium hyaluronate was injected intraarticularly at 1, 2, 3 weeks after surgery. Each patient was assessed with Lysholm knee joint score system before and after operation. RESULTS AND CONCLUSION:Eighty-one patients were fol owed up for an average of 1.5 years and five patients were lost to fol ow-up. Joint locking, significant tenderness and movement disorder symptoms disappeared in 79 of 81 patients, and meniscal healing was shown on MRI review within 1 year after surgery;the Lysholm score was higher than 75 points, and the excel ent rate was 98%. Only the remained two patients felt swel ing and tenderness and the movement was slightly restricted when going down or standing up. The Fast-Fix combined with sodium hyaluronate in meniscal repair under arthroscope has good effect and good function of knee joint.