1.Correlation between eystathionine βsynthase T833C gene polymorphism and ischemic cerebral vascular disease
Yuqiang SONG ; Fangqing LIU ; Chen ZHANG
Chinese Journal of Neurology 2008;41(11):731-733
Objective To investigate the correlation between cystathionine β-synthase (CBS) T833C gene polymorphism and the plasma homocysteine (Hey) levels in patients with ischemic cerebral vascular disease(ICVD). Methods Three hundred and sixty patients with ICVD and 210 control subjects were enrolled. The T833C polymorphism of CBS gene was analyzed by the amplification refractory mutation system (ARMS). Plasma Hey levels were measured by high performance liquid chromatography-fluorescence detection(HPLC-FD). Results Plasma Hey levels in the ICVD group ((17.6±4.8) μmol/L) were higher than those in control group ((13.3±4.3) μmol/L, t = 10.716, P< 0.05) . There were no differences in genotype frequencies and allele frequencies between the ICVD group and contral group (χ2 = 0.785, 0.941 ,P>0.05). Plasma Hey levels in CC genotype or TC genotype were higher than those in TT genotype (F = 6.56, P< 0.05). Condusions High plasma Hey level is an independent risk factor of ICVD,but CBS T833C polymorphism may not associated with ICVD.
2.Imaging diagnosis of extramedullary hematopoiesis
Lingling LIU ; Fei MIAO ; Cong ZHOU ; Jing XIE ; Fangqing GE ; Xiaoxing TANG
Journal of Practical Radiology 2014;(12):2013-2015,2083
Objective To discuss and analyze the imaging features of extramedullary hematopoiesis (EMH).Methods Imaging data of EMH in 7 patients with 85 lesions confirmed by clinical and pathological results were retrospectively analyzed.Results CT or MRI showed thoracic paravertebral multiple tumor-like soft tissue masses in 6 patients accompanied with pleural mass in 1,with liv-er mass in 1 and spleen mass in 1,and the masses in the distal ileum and in front of the inferior vena cava in 1.Thoracic paraverte-bral and pleural masses presented isodensity on plain CT and moderate enhancement on post-contrast CT.Among the 6 liver mas-ses,CT showed uniform density in 5 and small area of necrosis in 1.The only one spleen mass also appeared inner necrosis and mul-tiple small calcifications.The mass in the ileum presented thickened bowel wall with moderate enhancement,meanwhile the mass in front of vena cava presented obvious edge enhancement.MRI in 2 patients showed the masses with low signal on T1 WI and high sig-nal on T2 WI.Conclusion EMH has certain specific imaging characteristics,which may be helpful for correct diagnosis of EMH when combined with the clinical data.
3.Characterization of human αβTCR repertoire and discovery of D-D fusion in TCRβ chains.
Peipei LIU ; Di LIU ; Xi YANG ; Jing GAO ; Yan CHEN ; Xue XIAO ; Fei LIU ; Jing ZOU ; Jun WU ; Juncai MA ; Fangqing ZHAO ; Xuyu ZHOU ; George F GAO ; Baoli ZHU
Protein & Cell 2014;5(8):603-615
The characterization of the human T-cell receptor (TCR) repertoire has made remarkable progress, with most of the work focusing on the TCRβ chains. Here, we analyzed the diversity and complexity of both the TCRα and TCRβ repertoires of three healthy donors. We found that the diversity of the TCRα repertoire is higher than that of the TCRβ repertoire, whereas the usages of the V and J genes tended to be preferential with similar TRAV and TRAJ patterns in all three donors. The V-J pairings, like the V and J gene usages, were slightly preferential. We also found that the TRDV1 gene rearranges with the majority of TRAJ genes, suggesting that TRDV1 is a shared TRAV/DV gene (TRAV42/DV1). Moreover, we uncovered the presence of tandem TRBD (TRB D gene) usage in ~2% of the productive human TCRβ CDR3 sequences.
Complementarity Determining Regions
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genetics
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DNA Primers
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chemistry
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genetics
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Female
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Gene Rearrangement, beta-Chain T-Cell Antigen Receptor
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genetics
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Gene Rearrangement, delta-Chain T-Cell Antigen Receptor
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genetics
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Genes, T-Cell Receptor beta
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genetics
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Genetic Variation
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High-Throughput Nucleotide Sequencing
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Humans
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Immunoglobulin Joining Region
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genetics
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Immunoglobulin Variable Region
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genetics
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Male
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Receptors, Antigen, T-Cell, alpha-beta
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genetics
4.Phylogenomics of non-model ciliates based on transcriptomic analyses.
Xiao CHEN ; Xiaolu ZHAO ; Xiaohui LIU ; Alan WARREN ; Fangqing ZHAO ; Miao MIAO
Protein & Cell 2015;6(5):373-385
Ciliates are one of the oldest living eukaryotic unicellular organisms, widely distributed in the waters around the world. As a typical marine oligotrich ciliate, Strombidium sulcatum plays an important role in marine food webs and energy flow. Here we report the first deep sequencing and analyses of RNA-Seq data from Strombidium sulcatum. We generated 42,640 unigenes with an N50 of 1,451 bp after de novo assembly and removing rRNA, mitochondrial and bacteria contaminants. We employed SPOCS to detect orthologs from S. sulcatum and 17 other ciliates, and then carried out the phylogenomic reconstruction using 127 single copy orthologs. In phylogenomic analyses, concatenated trees have similar topological structures with concordance tree on the class level. Together with phylogenetic networks analysis, it aroused more doubts about the placement of Protocruzia, Mesodinium and Myrionecta. While epiplasmic proteins are known to be related to morphological characteristics, we found the potential relationship between gene expression of epiplasmic proteins and morphological characteristics. This work supports the use of high throughput approaches for phylogenomic analysis as well as correlation analysis between expression level of target genes and morphological characteristics.
Ciliophora
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genetics
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metabolism
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Phylogeny
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RNA, Protozoan
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genetics
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metabolism
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Transcriptome
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physiology
5.Expert consensus on microbiome sequencing and analysis.
Yunfeng DUAN ; Shengyue WANG ; Yubao CHEN ; Ruifu YANG ; Houkai LI ; Huaiqiu ZHU ; Yigang TONG ; Wenbin WU ; Yu FU ; Songnian HU ; Jun WANG ; Yuhua XIN ; Fangqing ZHAO ; Yiming BAO ; Wen ZHANG ; Juan LI ; Ming ZENG ; Haitao NIU ; Xin ZHOU ; Yan LI ; Shenghui CUI ; Jing YUAN ; Junhua LI ; Jiayi WANG ; Donglai LIU ; Ming NI ; Qing SUN ; Ye DENG ; Baoli ZHU
Chinese Journal of Biotechnology 2020;36(12):2516-2524
In the past ten years, the research and application of microbiome has continued to increase. The microbiome has gradually become the research focus in the fields of life science, environmental science, and medicine. Meanwhile, many countries and organizations around the world are launching their own microbiome projects and conducting a multi-faceted layout, striving to gain a strategic position in this promising field. In addition, whether it is scientific research or industrial applications, there has been a climax of research and a wave of investment and financing, accordingly, products and services related to the microbiome are constantly emerging. However, due to the rapid development of microbiome sequencing and analysis related technologies and methods, the research and application from various countries have not yet unified on the standards of technology, programs, and data. Domestic industry participants also have insufficient understanding of the microbiome. New methods, technologies, and theories have not yet been fully accepted and used. In addition, some of the existing standards and guidelines are too general with poor practicality. This not only causes obstacles in the integration of scientific research data and waste of resources, but also gives related companies unfair competition opportunity. More importantly, China still lacks national standards related to the microbiome, and the national microbiome project is still in the process of preparation. In this context, the experts and practitioners of the microbiome worked together and developed the consensus of experts. It can not only guide domestic scientific research and industrial institutions to regulate the production, learning and research of the microbiome, the application can also provide reference technical basis for the relevant national functional departments, protect the scale and standardized corporate company's interests, strengthen industry self-discipline, avoid unregulated enterprises from disrupting the market, and ultimately promote the benign development of microbiome-related industries.
China
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Consensus
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Humans
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Industry
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Microbiota