Background and purpose:Cyclooxysenase-2(COX-2)was first researched in colon caner,and extensively studied in breast cancer.However it was rarely reported in lymph node negative breast cancer.This study assessed the effect of expression of COX-2 in the prognosis of lymph node negative breast cancer and its use as information of COX-2 status to perfect the prognostic mathematic model.Methods:The expression of COX-2 protein was detected by IHC in 119 cases of lymph node negative breast cancer,the survival data was analyzed.Results:Elevated expression of COX-2 was associated with unfavorable distant disease-free survival(74% vs 93.86%).In subgroups by the simple prognostic model PI=size+HER2-2.5?age-CT-0.55?(ER?ET),the 5-year DFS were decreased with subgroups of low-risk,mid-risk and high-risk and the rate of elevated expression of COX-2 was increased with higher risk subgroups.Furthermore,in mid-risk group,the elevated expression of COX-2 was still able to predict poorer prognosis.Conclusion:The information of expression of COX-2 can be used to perfect the simple prognostic mathematic model,thus it would be more effective for treatment of breast cancer with auxiliary lymph nodes metastases.

Decoy receptor 3 (DcR3) is a newly discovered member of the tumor necrosis factor receptor (TNFR) superfamily, expresses highly in the digestive system neoplasms. DcR3 inhibits cellular apoptosis and produces cellular modulation by competitively combining with FasL, LIGHT and TL1A, therefore it deeply relates to the digestive system neophlasm's generation and progression. DcR3 is supposed to be the new tumor-specific marker that may cast the light to the digestive system neoplasms' generation, diagnosis,treatment, prognosis and effect observation. DcR3 is expected to open a new chapter in clinical application.

Objective To understand the body burden and the distribution characters of organochlorine pesticides and residues in non-occupational exposure population. Methods A cross-section study was conducted from Mar. 2004 to Jul. 2004, 107 men and 142 women, who were all healthy community residents, were investigated. Serum DDTs and HCHs level were determined by EC-ECD. The relationship between organochlorine pesticides in serum and age and BMI were analyzed by liner regression. Results Organochlorine pesticides and residues in serum with high detection rate were ?-HCH≥82.2%, p,p'-DDE≥94.4%, p,p'-DDT≥91.6%, and the level of the three organochlorine pesticides in the serum of women was significantly higher than that in men Mann-Whitney Test, P

Purpose: Comprehensively assess the role of prognostic and predictive factors and set up a mathematical model to evaluate prognosis and to help the decision of adjuvant therapy. Methods: All prognostic indicator and therapeutic modalities have been performed, mutivariate survival analyses was done by Cox regression. The split-group method was used to construct the mathematical model for prognosis. Results: In multivariate analysis, the factors including age, tumor size, overexpression of Her-2, the interaction between ER status and endocrine therapy (ER ET), and chemotherapy can independently predict prognosis. Finally, the mathematical model acquired is PS = Size + Her2 -2. 5 x age - CT -0. 55 x ( ET x ER). Conclusions: According to PI of individuals, the different risks of failure can been distinguished.

Objective: To observe the relationship between homocysteine(Hcy)and spatial cognitive ability and to evaluate whether vitamin B12 supplementation could protect spatial cognitive ability in rats. Methods: The rats were randomized into three groups. The experimental group was given methionine subcutaneously. The intervention group was given methionine and vitamin B12 subcutaneously. The control group received isometric normal saline. After being raised for 8 weeks, all rats were examined for plasma Hcy and serum vitamin B12 and scores of Y-maze test. The brain tissues of hippocampus were checked immunohistochemically for acetylcholinesterase (AChE). Results: The experimental group developed hyperhomocysteinemia and descreased spatial cognitive ability significantly (P0.05). Conclusion: Homocysteine can impair the spatial cognitive ability in rats and vitamin B12 is effective in lowering Hcy level and protect the spatial cognitive ability of rats.

Objective To explore the relationship between the tiny enhanced foci in hematoma and hematoma expansion at the acute stage of intracerebral hemorrhage (ICH).Methods CT routine and enhanced scan were applied in 36 ICH patients which onset ≤5 h,and CT examined again follow-up 1 or 2 d.The neurological function was evaluated by European Stroke Scale(ESS)at the 1st d and 21st d after onset.Results The tiny enhanced foci in hematoma were found in 11 csaes by the first CT scane.Follow up CT scane,the hematoma expansion was occurred in 10 cases,including 8 cases with tiny enhancing foci in hematoma.The incidence of hematoma expansion in ICH patients with tiny enhancing foci in hematoma(8 cases,72.7%) was significantly higher than in ICH patients without tiny enhancing foci in hematoma(2 cases,8.0%)(P

The presence of the cervical artery sulcus ring was found on the atlas in 56 out of 346 air pilots. The X ray appearance could be classified as follows. Type Ⅰ:Complete sulcus ring in 32 cases, in whom the sulcus might be complete unilaterally or bilatrally. Type Ⅱ: Incomplete or half ring in 18 cases. This type could be further divided into Ⅱ a , consisting of 4 persons with half ring in the anterior aspect, andⅡ b , half ring posteriorly in14 persons. Type Ⅲ: Broken ring in 6 persons. It is an anatomic variation, which may be have some relationship to vertigo. It is suggested that the presence of cervical artery sulcus ring on atlas should be taken as an disqualification factor in air pilot recruitment.

Objective To investigate the left atrium (LA) function and structure changes in the paroxysmal atrial fibrillation (AF) patients after catheter ablation using tissue Doppler imaging. Methods After complete pul-monary vein, radiofrequency ablation guided by Ensite NavX System, LA systolic function and LA diameter, volume, mean mitral gradient and mitral annulus early and advanced diastolic peak velocity were assessed in 32 cases of par-oxysmal AF patients,which were compared with age-matched controls before and after 24 hours, 1 week, 1 month) AF ablation. Results AF did not occur again in 32 AF paroxysmal patients after isolation. LA diameter and volume in AF groups before ablation were larger than controls(P<0.01), which were also larger 24 hours after ablation than before (P>0.05 ), but LA volume was larger than before (P<0.05), and decreased in I week after ablation (P< 0.05), but had no significant difference compared with controls in 1 month(P>0.05 ) ;mitral annulus advanced di-astolic peak velocity decreased in AF groups before ablation (P<0.01 )and was lower than that 24 hours after cathe-ter ablation (P<0.05 ), but increased after 1 week (P<0.05 or P <0.01 ), and had no significance after 1 month. Conclusion Catheter ablation is the effective way to manage AF because LA is distended and atrial systolic func-tion is reduced within 24 hours after procedure, then gradually increased in a week and will nearly recover to that be-fore procedure in a month, which may be correlated with LA repair, implicating that postprocedural thromboembollc risk and procedure injury should be taken into consideration.

ObjectiveTo detect methylation status of the 5'CpG island locating in the promoter region of p27 gene in gastric carcinoma and to analyze the relationship between the aberrant methylaion of p27 gene and the bionomics of gastric carcinoma.MethodsMethylation-specific PCR were used to detect the methylation status of p27 in gastric carcinoma( n =49), adjacent tissue of carcinoma ( n =20) and normal tissue(n =10), respectively.ResultsThe mathylation rate is 49％ (24/49) for gastria cancer, 25％(5/20) for tumor-sideward mucosa and 0 (0/10)for normal mucosa.There was a significant positive correlation between gastric carcinoma specimens and normal mucosa specimens ( P ＜ 0. 05 ). There was no significant diference between normal gastric group and tumor-sideward mucosa group ( P ＞ 0. 05 ) and no significant diference between tumor-sideward mucosa group and tumor group ( P ＞ 0. 05 ). The mathylation rate of p27 gene with lymph node metastases (61％) are higher than those without metastasis (28％) (P ＜ 0. 05). And there is no relation on patients' age, sex, tumor size and location.Conclusionp27 gene methylation of the p27 gene is correlated to oncogenesis and metastasis of gastric carcinoma and could lead to the loss of p27mRNA transcription.

Objective To study the right ventricular function of patients with pulmonary thromboembolism by using color Doppler ultrasound.Methods 31 patients with acute pulmonary thromboembolism,compared with 31 vohnteers,were enrolled in this study.The right ventricular anterior wall movement,right ventricular end diastolic volume,right ventricular ejection fraction,and myocardial performance index were observed by echocardiography.Resuits The right atrium diameter,right ventricles diameter,right ventrieular end diastolic volume and pulmonary artery inner diameter in study group were much larger than that in control group (P<0.01),and the right ventricular anterior wall movement and right ventrieular ejection fraction decreased in study group (P<0.01).Tricuspidal annular E peak velocity tended to be decreased,isovolumie relaxation time and isovolumic contraction time were prolonged and myocardial performance index was increased (P<0.01).Right ventricular myocardial performance index showed significant correlation with right ventrieular ejection fraction (r=0.78,P<0.01),isovolumic relaxation time and isovolumic contraction time(rl=0.88,r2=0.57,P<0.01).Conclusion The right ventricular function in patients with pulmonary thromboembelism is decreased and myocardial performance index is a sensitive index which can be used to reflect right ventricular function in pulmonary thromboembolism.