Objective To study changes of auditory brainstem response and expression of glutamate decar-boxylase-67(GAD67) in the auditory cortex in rats following noise exposure .Methods Rats were exposed to a-bove 4 kHz ,100 dB SPL white noise for 2 hours ,then auditory brainstem responses were measurey immediately , and at the 7th and 14th day post-exposure ,and marked GAD67-positive neurons in auditory cortex with immuno-histochemical techniques ,compared with the expression of GAD67 of exposed group to the control .Results ①The auditory threshold for clicks in all rats exposed to noise showed an initial threshold shift ,returned to near control levels at the day 14 .②GAD67-positive neurons 2~4 hours post-exposure increased by 105% than those of in the control group (P<0 .01) ,Then ,at the 7th and 14th day in exposed group were significantly lower than those of in the control group (P<0 .05) .Conclusion These studies describe a temporary threshold shift and increase markers for GAD67 immediately following acoustic exposure ,followed by a decline to below control levels .It may be closely related to noise deafness and other series of auditory dysfunction .

This study was aimed to set up the fingerprint chromatograms of chemical principles of Dendrobii Caulis by HPLC-DAD-ELSD. This analysis was performed at 30°C on a Dikma Cl8 column (250 mm × 4.6 mm, 5 μm) with a mobile phase consisting of acetonitrile-0.5% glacial acetic acid, gradient elution, at the flow rate of 1.0 mL?min-1 with DAD and ELSD detection. The HPLC fingerprint of Dendrobii Caulis was established. And 9 fingerprint peaks were marked. The similarity among different species was low. This method was stable, reliable and repeatable. It is useful for the study on the relationship between chemical components and species of Dendrobii Cauli.

Cocaine,as a widely abused and highly addictive drug,has a serious impact on the physical and mental health of individuals and carries a certain degree of social harm and economic burden.Acupuncture can assist in the treatment of cocaine addiction with fewer side effects.However,a well-defined mode of stimulation is an important factor in elucidating the various mechanisms by which acupuncture treats disease.This paper summarizes the problems in the mechanism of cocaine addiction,such as different parameters of stimulation,unstable depth of acupuncture,different acupoint selection,and different lengths of acupuncture time.To standardize the intervention measures of acupuncture experiments,it is suggested that in future research,the stimulation method should explore the best parameters,the selection of acupoints should be based on clinical practice,the timing of acupuncture should be objective,and the treatment course should consider the effects of acupuncture.