Objective To compare the short-term effect of Docetaxe and Irinotecan combined with Xeloda in treatment of end stage gastric cancer.Methods 65 patients with advanced gastric cancer were randomly divided into two groups.33 patients in group A received Docetaxe plus Xeloda and 32 patients in group B received Irinotecan plus Xeloda.At least two treatment cycles were completed.Results The overall response rate is 54.54%(A) and 53.13%(B),and the median progression-free survival was 6.1 months and 6.2 months.Patients in group A experienced more anemia,and patients in group B,diarrhoea.Conclusion The effects of Docetaxe or Irinotecan combined with Xeloda in the treatment of advanced gastric cancer are comparable and well tolerated.

[ ABSTRACT] AIM:To investigate the effect of paricalcitol ( P) on renal tubulointerstitial fibrosis and the under-lying mechanisms in diabetic nephropathy ( DN) .METHODS:DN rat model was induced by a single intraperitoneal in-jection of streptozotocin after fasting.The animals were randomly divided into 2 groups: the DN rats in paricalcitol-inter-vened group ( group P) were injected intraperitoneally with paricalcitol dissolved in propylene glycol after the day when the model was induced successfully at a dose of 0.4μg/kg (3 times a week);the DN rats in DN group ( group D) were given isopyknic propylene glycol.Normal control group ( group C) was also set up.The samples of blood, urine and renal tissue were collected after intervention of paricalcitol for 12 weeks.The biochemical indexes were measured.The renal tissues were used for pathologic observation and determining the expression of transforming growth factor-β1 (TGF-β1), Wnt-4,β-catenin and Klotho by immunohistochemistry and Western blotting.In addition, the correlation among the above indexes was analyzed.RESULTS:(1) Scr, BUN and 24 h urine protein increased significantly in group D compared with group C, while decreased in group P compared with group D ( P<0.05 ) .( 2 ) The area of renal tubulointerstitial fibrosis in-creased in group D compared with group C, while decreased in group P compared with group D (P<0.05).(3) The ex-pression of Klotho decreased, while the expression of TGF-β1, Wnt-4 and β-catenin increased in group D compared with group C (P<0.05).Compared with group D, the expression of Klotho increased, while the expression of TGF-β1, Wnt-4 andβ-catenin decreased in group P (P<0.05).(4) The expression of Klotho was negatively correlated with the fibrosis area, TGF-β1, Wnt-4 andβ-catenin (P<0.05).CONCLUSION:Paricalcitol inhibits renal tubulointerstitial fibrosis in DN by promoting the expression of renal Klotho, and inhibiting Wnt/β-catenin signaling pathway activation and TGF-β1 synthesis.

Aim To investigate the influence of gly-cyrrhizic acid( GA) on cell proliferation and cell cycle and secretion of extracellular matrix ( ECM) , the level of fibronection ( FN) , type Ⅳ collagen ( C-Ⅳ) in the rats′ glomerular mesangial cells ( HBZY-1 ) cultured with advanced glycation endproducts ( AGEs ) . Meth-ods HBZY-1 were incubated in culture medium con-taining AGEs in the presence of GA and treated for 48 h. At the same time, the normal and control groups were established. Methylthiazoletetrazolium ( MTT) as-say was used to measure cell proliferation;cell cycle a-nalysis was performed using a flow cytometry; FN and C-Ⅳwere analyzed by enzyme-linked immunosorbent assay ( ELISA ) . Results Cell counts increased markedly in AGEs group. Cell cycle analysis showed that cell percentage of S phase increased, G1 reduced and PI increased from 35. 01% ± 4. 21% to 44. 93%± 0. 25% ( P <0. 05 ) . FN and C-Ⅳ secretion in-creased ( P <0. 05 ) . Whereas GA was added, cell counts decreased, and the percentage of S phase also decreased and G1 increased, PI decreased from 44. 93% ± 0. 25% to 42. 16% ± 1. 04% ( P<0. 05 ) . In addition, the secretion of FN and C-Ⅳ decreased ( P<0. 05 ) . Conclusion GA could prevent AGEs-induced HBZY-1 . GA may protect HBZY-1 from in-hibiting the abnormal cell proliferation, changing the cell cycle. GA can reduce the synthesis of FN and C-Ⅳ.

Objective To investigate mutations of oncogene K-ras in colorectal cancer tissues and the relationship between mutations of K-ras and clinicopathological characteristics in colorectal cancer.Methods Specimens of 90 patients with colorectal cancer were detected K-ras gene by direct sequencing.Then satistical analysis were used to analyzed the associations between K-ras mutation status and clinicopathological characteristics in colorectal cancer.Results 31 cases were detected mutation in 90 cases of colorectal cancer specimens.The total mutation rate of K-ras was 34.4 ％.There were 21 eases of single mutation occurred in codon 12.One case had double mutation in codon 12.9 cases mutation were occurred in codon 13.There were significant difference between K-ras mutation rate and the location of tumor (P =0.042).The frequency of 12/13 codon mutation was no correlated with age,gender,tumor size,dukes stage,hepatic metastases,location of tumor.Conclusion The rate of K-ras mutation is correlated with the location of tumor.

Objective:To explore the relationship between lifestyle,self-esteem and life satisfaction among Chinese adolescents.Methods:10 899 adolescents in middle schools and colleges from 9 provinces in China were investigated by using Chinese Adolescent Lifestyle Scale(CALS),Rosenberg Self-Esteem Scale(SES).Results:The distribution of adolescents in the four groups divided by lifestyle and life satisfaction scores were different regarding the area and grade of these students(?2=248.93,P

This paper reviewed the basic concepts of creativity and systematic perspective on creativity and systematically analyzed the connotation of creativity in doctoral candidates,thus summarizing the advantages of the development of creativity in doctoral candidates:physiological function,intellectual quality,non-intellectual quality and professional innovation that may help to unleash creativity,knowledgeable,dedicated,and innovative supervisors and a correspondingly suitable environment,which is of great importance to the researches on unleashing the creativity in doctoral candidates and creating innovative results.

Objective To evaluate the association between the concentration of particulate matter (PM2.5)and asthma attack in children.Methods The computer and databases were used to search the key wordsAsthma andChild were searched by means of the subject headings ( Asthma andChild) and free words (Asthma * , Child *andPediatric * etc.),moreover PM2.5 was searched on the basis of free words such asPM2.5, Particulate Matter *which were linked withAND in PubMed and Medline database.Furthermore,the key words asthma AND (Child OR Pediatric OR Infant) AND (PM2.5 OR Particulate Matter OR Inhalable Particles) for keywords were searched in CBM,CNKI,VIP and Wanfang databases.The literatures included those from the initial computer database up to those of December 2014 from computer database.Meanwhile manual research was added to screen literatures according to the standards of inclusion and exclusion.Newcastle-Ottawa Scale was employed to assess the quality of the included literatures including selection,comparability and outcomc.Data wcre synthesized and analyzed by Rcvman5.3 and Stata12.0 version for statistical analysis,heterogeneity test,sensitivity analysis,subgroup analysis,and so on.Results A total of 17 literatures involving 1326573 children were included.The findings from quality assessment involved 2 studies for 8 scores,2 for 7 scores,7 for 6 scores,5 for 5 scores,and 1 for 4 scores,respectively.The result of Meta-analysis showed that the combined the odds ratio (OR) of PM2.5 with childhood asthma attack was 1.06,and 95％ confidence interval(CI) was 1.03-1.08.Subgroup analysis showed that the OR on the asthma hospital visit equaled to 1.03 (95％ CI 1.01-1.06),and the OR on the asthma symptom equaled to 1.11 (95％ CI 1.06-1.16),moreover,subgroup analysis between developed and developing countries showed that the OR of the asthma on developing countries corresponded to 1.04 (95％ CI 1.02-1.06),and the OR of the asthma on developed countries corresponded to 1.06 (95％ CI 1.03-1.09).Conclusion There is a positive correlation between concentration of PM2.5 and asthma attack in children.

Objectives To evaluate the prognostic value of the coronary artery calcium (CAC) score in patients with stable angina pectoris (SAP) who underwent percutaneous coronary intervention (PCI). Methods A total of 334 consecutive patients with SAP who underwent first PCI following multi-slice computer tomography (MSCT) were enrolled from our institution between January 2007 and June 2012. The CAC score was calculated according to the standard Agatston calcium scoring algorithm. Complex PCI was defined as use of high pressure bal-loon, kissing balloon and/or rotablator. Procedure-related complications included dissection, occlusion, perforation, no/slow flow and emer-gency coronary artery bypass grafting. Main adverse cardiac events (MACE) were defined as a combined end point of death, non-fatal myo-cardial infarction, target lesion revascularization and rehospitalization for cardiac ischemic events. Results Patients with a CAC score>300 (n=145) had significantly higher PCI complexity (13.1%vs. 5.8%, P=0.017) and rate of procedure-related complications (17.2%vs. 7.4%, P=0.005) than patients with a CAC score≤300 (n=189). After a median follow-up of 22.5 months (4-72 months), patients with a CAC score≤300 differ greatly than those patients with CAC score>300 in cumulative non-events survival rates (88.9 vs. 79.0%, Log rank 4.577, P=0.032). After adjusted for other factors, the risk of MACE was significantly higher [hazard ratio (HR):4.3, 95%confidence inter-val (95%CI):2.4-8.2, P=0.038] in patients with a CAC score>300 compared to patients with a lower CAC score. Conclusions The CAC score is an independent predictor for MACE in SAP patients who underwent PCI and indicates complexity of PCI and proce-dure-related complications.

〔Abstract〕 The paper introduces the informatization construction status of biobank in Peking University People's Hospital, including construction scope, basic infrastructure, steps as well as informatization management system application status, elaborates the application effect.The informatization construction of biobank could shorten the time of operation and ensure the quality of the samples.

Aim To observe whether the activation of aldehyde dehydrogenase 2 ( ALDH2 ) can protect a-gainst diabetes induced liver injury in rat model, and analyze the role of JNK pathway in the liver protection induced by activation of ALDH2 . Methods All male SD rats were randomly divided into three groups: nor-mal control group ( Con ) , diabetes group ( DM ) and ethanol + diabetes group ( EtOH + DM ) . After 8 weeks, the fasting blood glucose ( FBG) level, glyco-sylated hemoglobin ( HbA1c) level, serum AST and ALT levels were measured. The changes of hepatic pa-thology were observed by hematoxylin and eosin ( HE) staining method. The protein expressions of ALDH2, JNK and p-JNK in liver tissue were measured. Result Compared with control group, in DM group, the lev-els of FBG and HbA1c, serum AST and ALT levels were increased significantly. The structure of liver mor-phology was destroyed, disarranged and unclear, the hepatocyte was swollen, and a large number of inflam-matory cells were infiltrated. ALDH2 protein expres-sion was decreased, while the expressions of JNK, p-JNK and the ratio of p-JNK/JNK were increased. Com-pared with DM group, in EtOH+DM group, the levels of FBG and HbA1c, serum AST and ALT levels were decreased. The expression of ALDH2 protein was in-creased, accompanying with the decrease of JNK, p-JNK protein expressions and the ratio of p-JNK/JNK. Conclusion Activation of ALDH2 can protect the liv-er against diabetes induced liver damage in rat model, which may be relevant with inhibiting the JNK path-way.