The possibility of using monoclonal antibody immunodot test as alternative method for poliovirus typing was evaluated by comparing the results of polyclonal reference serum neutralization test and those of type Ⅱ poliovirus monoclonal antibody 18-2-1 immunodot test, both for 7 type of enteroviruses, i.e.polioviruses type Ⅰ, Ⅱ, Ⅲ, Coxsackievirus A7, B4, Echovirus type 9 and enterovirus type 71,and for 10 wild strains of poliovi(?)us Preliminary results demonstrated both tests to be equal, which suggested that monoclonal antibody immunodot test could replace neutralization test as an alternative typing method.