1.Research progress on Drynaria fortunei naringin on inflammation and bone activity.
Fang-ming YIN ; Lian-bo XIAO ; Yun ZHANG
China Journal of Orthopaedics and Traumatology 2015;28(2):182-186
Flavonoid naringin is widely distributed in various types of plants and is an important component of herbal Drynaria. In previous studies, Drynaria has been demonstrated to have inhibitory effects on inflammatory responses and bone destruction and exert anabolic effects on bone, has been widely used in the clinical treatment. Naringin, was in the stage of experimental yet. The experimental results have confirmed that naringin suppressed inflammation including arthritis by lowering the expression of inflammatory cytokines, and the mechanism can be explained as reducing the expression of NF-κB. Naringin has been shown to increase osteoblast proliferation by increasing the expression of BMP-2, inhibit osteoclast activity by reducing the expression of RANKL. In animal experimental, naringin was useful for osteoporosis, and the mechanisms are in-depth studies. Research in the field of traditional Chinese medicine and orthopedics, naringin as a explicit material structure in the components of Drynaria, has been concerned about the experimental studies, it is not only prosperity the development of traditional Chinese medicine research,but also ready for clinical studies anti-inflammatory and bone effects of naringin in the future.
Animals
;
Anti-Inflammatory Agents
;
pharmacology
;
Bone and Bones
;
drug effects
;
Flavanones
;
pharmacology
;
Humans
;
Medicine, Chinese Traditional
;
Osteoblasts
;
drug effects
;
Osteoclasts
;
drug effects
;
Osteoporosis
;
drug therapy
;
Polypodiaceae
;
chemistry
2.Bone marrow edema resulting from nontraumatic femoral head necrosis:Association Research Circulation Osseous staging and pain grading
Weiqiang ZHAN ; Zongsheng YIN ; Ming LU ; Jialiu FANG ; Qin HU
Chinese Journal of Tissue Engineering Research 2015;(46):7529-7533
BACKGROUND:The relationship between the occurrence time and clinical symptoms of bone marrow edema in nontraumatic femoral head necrosis is stil controversial. OBJECTIVE:To evaluate the relationship of bone marrow edema in nontraumatic femoral head necrosis with Association Research Circulation Osseous (ARCO) staging and pain grading. METHODS: Forty-eight patients with nontraumatic femoral head necrosis (58 hips) were confirmed to have bone marrow edema on MRI T1 weighted images and T2 fat suppression images. Then, the patients were graded according to the MRI Classification Method reported by Zhao Pei-rong, the ARCO Staging System and Harris hip score for pain grading. We analyzed the correlation of bone marrow edema with ARCO staging and pain grading. RESULTS AND CONCLUSION:The incidence rate of bone marrow edema in ARCO I-III was increased in sequence, and the difference was statisticaly significant (χ2=27.69,P=0.001); the average rank of bone marrow edema in ARCO I-III was increased significantly (χ2=19.947,P=0.000). The incidence rate of bone marrow edema also increased among different pain grading, and the difference was statisticaly significant (χ2=57.0, P=0.000); the average rank of bone marrow edema among different pain grading was increased significantly (χ2=174.0,P=0.000). The presence and aggravation of bone marrow edema indicate the enlargement of necrosis area and the aggravation of symptoms in patients with nontraumatic femoral head necrosis, which can be used as an auxiliary index for the appraisal of disease progression.
3.Construction of Lentiviral Expression Vector Containing Extracellular Domain of Human Hepatocyte Growth Factor Receptor and Its Expression in 293T Cell.
Jia GUO ; Yanxin YIN ; Ming JIANG ; Lihua YU ; Yun JIANG ; Guiqing LI ; Jianmin FANG
Journal of Biomedical Engineering 2015;32(2):400-404
This research aims to construct a lentiviral expression vector carrying the extracelluar domain (ED) of human hepatocyte growth factor receptor (C-Met), and to express it in transfected 293T cells. The extracellular domain of C-Met was amplified by RT-PCR, ligated with lentiviral expression vector p RRL-CMV-ED, and then expressed in 293T cell line. The expressed protein was purified and identified by RT-PCR and Western blot. The enzyme digestion and sequence analysis showed that the lentiviral expression vector p RRL-CMV-ED was constructed correctly. The size of amplified genes was about 2 700 bp. The purified protein with Ni-affinity column was about 105 kD analyzed by SDS-PAGE. The Western blot and ELISA results showed that the expressed protein which could bind to HGF specifically was the extracelluar domain of human hepatocyte growth factor receptor. This research may lay a foundation for further study of anti-C-MET monoclonal antibody and neutralizing antibody.
Genetic Vectors
;
HEK293 Cells
;
Humans
;
Lentivirus
;
Proto-Oncogene Proteins c-met
;
genetics
;
metabolism
;
Transfection
4.Research on chemical reactions during ginseng processing.
Miao ZHANG ; Kun-Ming QIN ; Wei-Dong LI ; Fang-Zhou YIN ; Hao CAI ; Bao-Chang CAI
China Journal of Chinese Materia Medica 2014;39(19):3701-3706
As a kind of commonly used traditional Chinese medicine, ginseng has a high reputation at home and abroad. The research of ginseng has been expanded to medicine, pharmacy, biology, food science and other fields, with great achievements in recent years. Ginseng contains ginsenosides, volatile oil, carbohydrates, amino acids, polypeptides, inorganic elements and othser chemical constituents. Each component has extensive physiological activity, and is the base of ginseng's effect. After processing, the complicated changes are taken place in the constituents of ginseng, and some new substances produced. This paper aims to review the studies on chemical constituents and their mechanisms during ginseng processing, and the ideas, methods and the direction of the development of traditional Chinese medicine processing in the future.
Chemistry, Pharmaceutical
;
methods
;
Drugs, Chinese Herbal
;
chemistry
;
Panax
;
chemistry
;
Plants, Medicinal
;
chemistry
5.Screening for pathogenesis-related genes of osteosarcoma using gene microarray
Guo-Dong LI ; Zheng-Dong CAI ; Yin-Quan ZHANG ; Ming RU ; Fang JI ;
Chinese Journal of Cancer Biotherapy 2006;0(05):-
Objective:To screen for the pathogenesis-related genes of osteosarcoma and to assess their roles for the de- velopment of osteosareoma.Methods:Total RNA was extracted from 3 ATCC osteosarcoma cell lines and an osteoblastic cell line and was used to synthesize biotinylated cRNAs;the latter were hybridized to Affymetrix~(?)GeneChip~(?)U133A ar- rays and a gene with more than 2 folds of change was selected.Ten of the differentially expressed genes were chosen and the primers were designed and the synthesized.Then SYBR~(?)Green real-time PCR(RT-PCR)method was used to detect the expression of the 10 genes in 9 fresh osteosarcoma specimens.ABI Prism 7 000 system was used to analyze the differ- ent expression between osteosarcoma cell line and osteoblastic cell line.Results:We identified 58 up-regulated and 142 down-regulated genes in the 3 osteosareoma cell lines.Many of the genes were firstly reported to be related to the patho- genesis of osteosarcoma.These differentially expressed genes were mainly involved in energy and material metabolism,on- cogene,signal transduction gene,transcription- related genes,cell cycle-related genes,cell apoptosis-related gene,im- mune response gene,tumor suppressor genes,etc.The array results of 10 randomly selected genes were further verified by the RT-PCR in 9 fresh osteosarcoma specimens.Conclusion:Many genes are involved in the pathogenesis of osteosarcoma. Gene microarray can help to discover the genes related to the pathogenesis of osteosarcoma,which may lay a foundation for studying the molecular mechanism of osteosarcom.
6.Evaluation on outcome of iodine deficiency disorders control in Hubei Provinvce in 2007
Ming-jian, LI ; Qing, SHI ; Jian, YIN ; Bao-guo, HUANG ; Fang, ZHOU ; Pei-sheng, XIONG ; Xin-guo, GONG
Chinese Journal of Endemiology 2008;27(5):541-542
Objective To investigate the current situation of the prevention and contml of iodine deficiency disorders(IDD)in Hubei Province,SO as to provide a policy.making basis for controlling work of IDD. Methods Using the method of proportional population sampling(PPS),30 eounties were seiected in Hubei Province.In each selected county,1 primary school was chosen.In every primary school,40 pupil8 aged 8~10 years were selected to examine thyroid size,intelligent quotient(IQ),and salt iodine contents at their home.In the selected pupils,2 boys and 2 girls were chosen to determine their urinary iodine contents in each age grouD.Twenty pupils in the above school and 5 housewives ileal"to this school were tested in health education questionnaire. Results The median of salt iodine was 30.1 mg/kg,and the rate of comsuming qualified iodized salt was 96.2% (1154/1200).The rates ofchild goiter were 6.5%(78/1200)by palpation and 4.1%(49/1200)by B ultrasound.The median of urinary iodine Was 358.4μg/L and mean of the IQ was 105.3±14.4.The rate of qualified scores of both students and housewives Was 25.5%(153/600)、90.7%(136/150).Conclusions The current Bituation of iedine nutrition is good.The goal of eliminating IDD has been achieved in Hubei Province.
7.The change of immunoreactivity in glia cells and its sense by using early Parkinson's disease rat model.
Hong XU ; Fang-Yuan DONG ; Sheng-Ming YIN ; Dong-Mei WANG ; Yi-Ping SUN ; De-Qin YU ; Wan-Qin ZHANG
Chinese Journal of Applied Physiology 2012;28(1):71-93
Animals
;
Astrocytes
;
immunology
;
metabolism
;
Male
;
Microglia
;
immunology
;
metabolism
;
Neurons
;
metabolism
;
Oxidopamine
;
metabolism
;
Parkinson Disease
;
immunology
;
Rats
;
Rats, Sprague-Dawley
8.Primary cutaneous follicle centre lymphoma: report of a case.
Yin-fang RONG ; Lin-ming HE ; Yong-sheng ZHANG ; Yi-zhong FENG
Chinese Journal of Pathology 2013;42(11):769-770
Antigens, CD20
;
metabolism
;
Antineoplastic Combined Chemotherapy Protocols
;
therapeutic use
;
Burkitt Lymphoma
;
metabolism
;
pathology
;
CD79 Antigens
;
metabolism
;
Cyclophosphamide
;
therapeutic use
;
DNA-Binding Proteins
;
metabolism
;
Diagnosis, Differential
;
Doxorubicin
;
therapeutic use
;
Humans
;
Lymphoma, B-Cell
;
pathology
;
Lymphoma, Follicular
;
drug therapy
;
metabolism
;
pathology
;
Lymphoma, Large B-Cell, Diffuse
;
metabolism
;
pathology
;
Male
;
Middle Aged
;
Prednisone
;
therapeutic use
;
Proto-Oncogene Proteins c-bcl-6
;
Skin Neoplasms
;
drug therapy
;
metabolism
;
pathology
;
Vincristine
;
therapeutic use
9.Treatment of aneurysms rupture during endovascular embolization
Xu-Ying HE ; Chuan-Zhi DUAN ; Tie-Lin LI ; Qiu-Jing WANG ; Fang-Ming YIN ; Ru-Xiang XU ;
Journal of Interventional Radiology 1994;0(02):-
Objective To evaluate the treatment of aneurysms rupture during endovascular embolization.Methods Nine aneurysms ruptured during the embolization and were treated with endovascular embolization.The reasons of aneurysms rupture during embolization,the prevention and the first aid after aneurysms rupture were analysed.Results Seven patients recovered and 2 died.Conclusions The optimal treatment of aneurysms rupture during endovascular embolization is effective,(J Intervent Radiol,2007,16: 132-134)
10.Electroencephalogram nonlinear parameters for assessing the therapeutic efficacy in patients with acute carbon monoxide poisoning
Yin-Xu WANG ; Iao-ming X WANG ; Hui LIU ; Fang CHEN
Chinese Journal of Neuromedicine 2012;11(2):177-181
Objective To investigate the value of electroencephalogram (EEG) nonlinear parameters in assessing the therapeutic efficacy in patients with acute carbon monoxide poisoning (ACMP). Methods Twenty-one patients diagnosed with ACMP in our department from November 2010 to March 2011 and 21 healthy volunteers were enrolled in this study.The EEG nonlinear parameters were obtained fi om the patients for analysis on the day of admission, at 2 and 4 weeks post-treatment,respectively,including approximate entropy (ApEn),cross-approximate entropy (C-ApEn),correlation dimension (D2),point wise correlation dimension (PD2),mutual dimension (Dm),Lyapunov exponent (LE),Kolmogorov entropy (KE) and Complexity (Cx).The EEG nonlinear parameters of the patients were compared with those of the healthy volunteers. Results The mean values of D2, Cx,ApEn,C-ApEn and Dm were found to gradually increase with the duration of therapy, whereas the values of PD2,LE and KE had no association with the therapy.On the day of admission,the mean values of D2,Cx,ApEn, C-ApEn and Dm for ACMP patients were significantly different from those of the healthy volunteers (P<0.05),but at 2 and 4 weeks post-therapy,the mean values ofLE,PD2,D2,Dm,Cx,ApEn and C-ApEn of the ACMP patients were not significantly different from those of the healthy volunteers (P>0.05). Conclusion Since EEG nonlinear parameters (D2,DM,Cx,ApEn,C-ApEn) can objectively reflect the status of brain functional recovery in ACMP patients at an early treatment stage,they can serve as practical measures in the treatment of ACMP.