Child ; Herpesviridae ; classification ; Herpesviridae Infections ; diagnosis ; Humans ; Paramyxoviridae ; classification ; Paramyxoviridae Infections ; diagnosis ; Parechovirus ; classification ; Pediatrics ; Picornaviridae ; classification ; Picornaviridae Infections ; diagnosis ; SARS Virus ; classification ; Severe Acute Respiratory Syndrome ; diagnosis ; Virus Diseases ; diagnosis ; Viruses ; classification

Objective To examine whether electroacupuncture at the Wangu acupoint (GB 12 ) , whose position is similar to the cerebellar fastigial nucleus ,can reduce inflammatory cytokines in the hippocampus of rats with vascular dementia (VD) to provide theoretical evidence for treating VD with electroacupuncture .Methods Healthy Sprague‐Dawley rats ( n=54 ,300 -450 g) were randomly divided into three groups :sham surgery group ,VD group ,and electroacupuncture group .The ethologic scores of VD rats were evaluated and the mRNA expressions of inflammatory cytokines (TNF‐α,IL‐6 and IL‐1β) in the hippocampus were assessed and the hippocampal tissues were observed by hematoxylin‐eosin (HE) staining .Results Compared with VD group ,in electroacupuncture group the rats' learning ability improved significantly and the mRNA expressions of TNF‐α, IL‐6 and IL‐1β decreased . Simultaneously ,the damage extent of nerve cells in the hippocampal tissues decreased , and their morphology recovered to nearly normal .Conclusion Electroacupuncture at the Wangu acupoint can decrease the level of inflammatory cytokines in the hippocampus and reduce the damage extent of nerve cells in the hippocampus ,thus providing a new neuroprotective method for VD .

Objective:To study the significance of QT dispersion(QTd) changes in electrocardiogram(ECG) after coronary artery bypass graft. Methods:Forty eight patients scheduled for elective coronary artery bypass graft were studied. The changes of QTd were monitored by 12 lead electrocardiogram system before surgery (as baseline values), at the end of surgery, and 1 4 d and 7 d after surgery.Results:The baseline values for QTd was (56.32?2.25) ms.QTd were significantly longer as compared to the baseline values ( P

Galanin is a peptide with wide-ranging effects,especially within the central,peripheral nervous system and endocrine system.Many tumors of neuroendocrine origin,and also an increasing number of non-neuroendocrine cancers,have been shown to express galanin and/or its receptors.Expression of galanin peptide has been detected in pheochromocytoma,squamous cell carcinoma,pituitary adenoma,gastrointestinal cancers and so on.Galanin peptide plays an important role in tumori-genesis and progression of many kinds of tumors.Therefore,studying the roles of galanin and its receptors are helpful for diagnosis and treatment of tumors.

Objective To invstigate the effect of high dose X-ray irradiation on the expression of multidrug resistance-1 (MDR1), Bcl-2, MMP7 genes. Methods A nasopharyngeal carcinoma cell line,CNE1, were irradiated with a total dose of 50 Gy. The resistance to the cisplatin of CNE1 cells and the irradiated CNE1 (CNE1 R) cells was detected by MTT. mRNAs expression of MDR1 , Bcl-2 and MMP7 was measured by quantitative RT-PCR. Results The expression of MDR1 increased in CNE1 R cells. The semiquantitative A value of MDR1 mRNA was 0.47 ±0.04, and the value of CNE1R cells (1st, 7th, 21st,28th, 35th, 42nd and 49th days after irradiation) were 0.67 ± 0. 06 (t = -5.44, P = 0. 003) ,0.70 ± 0. 01(t=-5.90,P=0. 002),0.73±0. 01(t= -6. 45,P=0. 001) ,0. 67 ± 0. 03 (t= -3.97,P=0.011),0.65 ±0.01(t = -4.43,P=0. 007),0. 62±0. 05(t= -2. 64,P=0.046) and 0.62 ±0.02(t = -3.34,P=0.021), respectively. Bcl-2 mRNA expression were 0.55 ±0.02 and 1.05 ±0.04(t = -9.93,P=0. 000) and MMP7 mRNA expression were 0.51 ±0.01 and 0.82 ±0.02(t = -8.51,P=0.000) in CNE1and CNE1 R cells. Conclusions The MDR1 expression was increased after a total dose of50 Gy irradiation,which may be related to the synchronous change of Bcl-2 and MMP7 genes.

AIM:To study the pharmacological action of Zhikang Capsule (Herba et Radix, Paederiae scandentis, etc.) METHODS: To establish the model of anus injury in the rectal position on rabbit, anti-inflammation and analgesia effect in rats and mice were observed by carrageen and hot-plate method, and inflammatory mediator PGE_2 of rat paw edema was analysed. RESULTS: Zhikang Capsule had the obvious effect to help heal and recover the anus injury in the rectal position and it could inhibit rats’ thorax leukocytoplania and rat paw edema of Garrageenan-induced and cotton pellet-induced granuloma formation in mice. The exudation of blood capillaryies and the contents of inflammatory medium PGE_2 were lowered and improved in the pain threshold. CONCLUSION: Zhikang Capsule has the obvious effect on the healing and recovery of the anus injury in the rectal position, its anti-inflammiting effect is related to the decrease in the exudation of blood capillaries and the contents of inflammatory medium PGE_2.

Objective:To summarize the major risk factors of infection in the process of cleaning and disinfection of endoscopy center in our hospital,and formulate the corresponding prevention measures to reduce the incidence of endoscopic infection so as to ensure medical safety of patients.Methods:The specialist check the cleaning disinfection process,statistics of each link in the risk factors of infection.600 endoscopes were taken at random after disinfection,endoscopic inner cavity and the outer surface samples are collected,the cleaning and disinfection of the test results for evaluating quality indicators.Results:Testing 600 samples of qualified 584,and the percent of pass is 97.3％,gastroscopy,colonoscopy,duodenum mirror,bronchoscope,endoscopic ultrasonography qualified rate was 98.6％,96.8％,95.6％,96.0％,97.2％,respectively.28 strains of pathogenic bacteria were detected in 16 cases of unqualified samples,including 13 strains of helicobacter pylori,7 strains of escherichia coli,4 strains of pseudomonas aeruginosa,2 strains of pseudomonas aeruginosa,1 strains of staphylococcus aureus,1 strains of Klebsiella pneumoniae;16 cases to detect unqualified reason analysis,scrub incomplete accounted for 37.5％,Not according to the instructions required to use multi-enzyme and disinfectant accounted for 18.75％,special infected patients did not do special treatment of endoscopy accounted for 12.5％,endoscopic insufficient drying and the staffhand hygiene is not up to standard accounted for 12.5％,respectively.Conclusion:Endoscopic cleaning and disinfection aspects of infection caused by many factors,infection control education should be strengthened for all personal in the endoscopy center,regular professional training of cleaning and sanitation personal,cleaning and disinfection process should be standardized management,establish traceability registration system,control the quality of the endoscope cleaning and disinfection from each link.It is the main measure to prevent digestive endoscopy center in infection.

Objective To observe the clinical efficacy of heat-clearing, dampness-removing, and Qi-blood-tonifying Chinese herbal medicine compound for the treatment of recurrent genital herpes(RGH), and to investigate the expression levels of Th17 cells and Treg cells in peripheral blood of patients with RGH as well as their role in the pathogenesis of RGH. Methods Sixty RGH patients were randomly divided into experimental group and control group, 30 cases in each group. The experimental group was treated with Chinese medicine decoction, and the control group was treated with Aciclovir tablets orally. The treatment course covered 6 months. The percentages of Th17 cells and Treg cells in the peripheral blood of the RGH patients were detected with flow cytometry. Results (1) After treatment for 3, 6 months, the total effective rate of the experimental group was 53.33%, 80.00%, and that of the control group was 23.33%, 46.67% respectively. There were significant difference between the two groups(P < 0.05).(2) After treatment, the percentage of Th17 cells and the ratio ofTh17/Treg cells were decreased while the percentage of Treg cells was increased in the two groups, and the differences before and after treatment were significant in the experimental group(P<0.05) while were insignificant in the control group (P > 0.05). The inter-group comparison showed the decrease of Th17 cell percentage and Th17/Treg cell ratio as well as the increase of Treg cell percentage in the experimental group was superior to that in the control group, the differences being significant (P<0.05). Conclusion The curative effect of Chinese herbal compound for RGH is better than that of the control group. Chinese herbal medicine compound can decrease the level of Th17 cells, increase the level of Treg cells, thus to decrease the ratio of Th17/Treg cells, indicating that heat-clearing, dampness-removing, and Qi-blood-tonifying Chinese herbal medicine compound can correct the imbalance of Th17/Treg cells, and then plays a role in anti-inflammation and immune regulation.

To find a way to improve the effects of clinical health education,the clinical health education nurses collocating qualifications to hold their posts, job duties, examinations of training, and practical efficacies were recommended. The setup of clinical education nurses had the advantages as follow: First, the clinical health education with high levels and high qualities can effectively strengthen the patients attending consciousness;second,scientific trainings of health education nurses were useful to pre control the qualities of the health education and improve the level; At last, respecting and caring for patients were the key points to make the patients satisfy the health education.

Objective:To discuss the effect of individualized physical exercise prescription on patients’ nutrition status, physical ability and self-care ability in patients with intestinal fistula and severe malnutrition. Methods:Twenty-four patients were divided randomly into two groups, the experiment group exercised according to physical exercise prescription, control group exercised in a routine way. The patients’ weight, physical ability and self-care ability were measured on the day before exercise and the 28th day after exercise. Results:Patients in the exercise group had significant more exercise than those in the routine group, and the physical ability and self-care ability were significant better that those in the routine group. Conclusion:Individualized physical exercise prescription can accelerate the recovery of physical ability and self-care ability, it can be used safely and effectively in patients with intestinal fistula and severe malnutrition.