OBJECTIVE:To investigate the clinical etiology,serum type and drug resistance of lung cancer complicated with pulmonary Haemophilus influenzae (Hi) infection,in order to provide reference for infection prevention and rational drug use. METHODS:Clinical data of 8025 inpatients with lung cancer complicated with pulmonary infection in our hospital from Jan. 2009 to Jun. 2016 were collected,and the clinical etiology of pulmonary Hi infection was analyzed. The slide agglutination method was used for serotyping,nitrocefin slip method was used to detect β-lactamase,K-B method was used for drug sensitivity test,WHO-NET 5.6 software was used to deal with the results of drug sensitivity test. RESULTS:Eleven factors as age,clinical classifica-tion,invasive operation,surgery,radiotherapy and chemotherapy,hospitalization time,use of broad-spectrum antibiotics or hor-mones and other were closely related to pulmonary Hi infection in lung cancer patients(P<0.05 or P<0.01). Among 8025 speci-mens,104 strains of Hi were detected with detection rate of 1.30%. Serum type NTHi accounted for 44.23%. Separable strains were mainly b type(22.12%),followed by f type(17.31%)and a type(11.54%). Among 104 strains of Hi,56 strains of β-lac-tamase were detected with enzyme-producing rate of 53.85%. Drug resistance of 104 strains of Hi to ampicillin,amoxicillin,com-pound sulfamethoxazole and chloramphenicol were all higher than 35%;drug resistance of Hi toβ-lactamase inhibitors,cephalospo-rins,carbapenems,azithromycin and other antibiotics were all lower than 20%. Drug resistance of β-lactamase producing stains to ampicillin,amoxicillin,compound sulfamethoxazole and chloramphenicol were all higher than those of non-producing strains,with statistical significance (P<0.01). There was no statistical significance in drug resistance to other antibiotics between producing stains and non-producing strains(P>0.05). CONCLUSIONS:The clinical etiology of pulmonary Hi infection in patients with lung cancer is complicated. The serum type of the isolate is mainly NTHi;enzyme production situation is not optimistic,but Hi keeps a low rate of drug resistance to most antibiotics. β-lactamase inhibitors,cephalosporins,carbapenems and azithromycin are first choice for the treatment of Hi infections.

objective To investigate the changes in angiotensin-Ⅱ (AT-Ⅱ), endothelin (ET) andcalcitonin gene-related peptide (CGRP) during clipping of intracranial aneurysm performed under desfiuraneanesthesia. Methods Forty-five ASAⅠ -Ⅱ patients (20 male, 25 female) aged 14-66yr undergoing electiveclipping of intracranial aneurysm two weeks after acute subarachnoid bleeding. Anesthesia was induced withmidazolam 0 .05 mg?kg~(-1), fentanyl 2?g?kg~(-1), propofol 2 mg?kg~(-1) and vecuronium 0.1 mg?kg~(-1). The patientswere mechanically ventilated after tracheal intubation and P_(ET)CO_2 was maintained at 30-35 mm Hg. Anesthesia wasmaintained with desflurane at 1 .0-1 .5 MAC with low flow (0.3-0.5 L?min~(-1) ) supplemented with intermittentintravenous boluses of vecuronium. Radial artery was cannulated for direct continuous BP monitoring. ECG, HR,SPO_2 and P_(ET) CO_2 were continuous monitored during operation. Blood samples were taken before induction ofanesthesia (T_1, baseline), when dura was opened (T_2 ), while aneurysm was being clipped (T_3 ) and 30 min afterclipping for determination of plasma concentrations of AT-Ⅱ, ET and CGRP. Results Plasma concentration ofAT-Ⅱ did not change significandy throughout the surgery. Plasma ET concentration decreased significantly at T_2and T_3 compared with the baseline (P

OBJECTIVETo explore the impact and effect mechanism of electroacupuncture (EA) on oocyte quali ty in the patients with infertility of kidney deficiency pattern.

METHODSSixty-six cases differentiated as kidney de ficiency and with in vitro fertilization-embryo transplantation (IVF-ET), aged fromnt 35 to 42 years were rando- mized into an observation group and a control group, 33 cases in each one. The IVF-ET therapy of the long proto- col with gonadotrophin releasing hormone agonist was adopted in the two groups. In the observation group. on the 5th day of menstruation in IVF cycle, EA was applied to Sanyinjiao (SP 6). Zigong (EX CA 1), Zhongji (CV 3) and Guanyuan (CV 4). In the control group, the sham-acupuncture was applied to the same acupoints. The treatment was given once every two days till the date of egg collection and the needles were retained for 30 min each time. The change in the score of kidney deficiency syndrome, the high-quality oocyte rate, the high-quality embryo rate and clinical pregnant rate were observed in the two groups. The levels of insulin-like growth factor-i (IGF-1) and IGF-2 in follicular fluid and the serum β-endorphin β-EP) on the date of egg collection and the correlation with oocyte quality were compared bIetween the two groups.

RESULTS1) In the observation group, the kidney deficiency syndrome score after treatment was reduced apparently as compared with that before treatment (P<0. 05), the score after treatment in the observation group was reduced much more apparently as compared with the control group (P<0.05). 2) The high-quality egg rate and the high-quality embryo rate in the observation group were both higher than those in the control group [81.3% (161/198) vs 57.6% (98/170), 59.8% (58/97) vs 37.7% (26/69), both P<0.05]. 3) Compared with the control group. the levels of IGF-1 and IGF-2 in follicular fluid and serum β-EP on the day of egg collection were all increased obviously in the observation group (all P<0. 05). 4) The levels of IGF-1 and IGF-2 in follicular fluid and serum β-EP presented the linear positive correlation with the high-quality egg rate.

CONCLUSIONEA effectively improves the expressions of IGF in follicular fluid and serum β-EP, increases the high-quality egg rate and high-quality embryo rate and relieves the symptoms of kidney deficiency.

Acupuncture Points ; Adult ; Electroacupuncture ; Female ; Fertilization in Vitro ; Humans ; Infertility, Female ; metabolism ; physiopathology ; therapy ; Insulin-Like Growth Factor I ; metabolism ; Kidney ; physiopathology ; Oocytes ; Pregnancy ; beta-Endorphin ; metabolism

Objective To understand the iodine nutrition of children in Qiandongnan and to provide the scientific evidence for prevention and control of iodine deficiency disorders (IDD). Methods The 3200 urinary samples of pupils aged 8-10 were collected from 80 primary schools in 16 county and city in Qiandongnan in 2006. The urinary iodine content was tested by colorimetric ceric-arsenic assay and vitriolic ammonium assimilation. Results The median of urinary iodine content in children samples was 362.9?g/L, the percentage of urinary iodine( ≥100?g/L) was 98.00%, in which ≥300?g/L was 65.31%, the percentage of urinary iodine(

Objective:To construct a monoclone cell line expressing M-HBsAg.Methods:Transfected SP2/0 with pRc/CMV-S 2S by Calcium Phosphate Method,selected the cells by G418,detected HBsAg by Dot-blot、ELISA and Western-blot hybridization.Results:By selection of G418,G418 resistent cells were obtained;tests of Western-blot hybridization and ELISA demonstrated their expression of aimed protein.Conclusion:Monoclone Cell Lines of SP2/0-S 2S were obtained.

Objective To study the diagnosis,management and prognosis of persistent m?llerian duct syndrome (PMDS). Methods 2 cases of PMDS associated with transverse testicular ectopia were reported.Clinically there was azoospermia or testis tumor.Corporeal hysterectomy and orchidopexy were performed and the testis tumor was excised. Results Vascular supply and hormonal function of the testis were normal in both 2 patients after 2 year's follow up. Conclusions PMDS is an inherited male pseudohermaphroditism. Every effort should be made to preserve the testis and its fertility function when surgical management is needed.The possible development of testis tumor should be kept in mind and closely observed.

Objective To discuss the feasibility,indications,surgical techniques,and outcome con- cerning the application of less invasive stabilization system (LISS) for complex proximal femoral fractures.Methods We treated 12 complex proximal femoral fractures using femoral LISS reversely from June 2005 to May 2006. They were five complex intertrochanteric fractures and seven subtrochanteric ones.By AO classification,two were type 31-A2.2,two type 31-A2.3,one type 31-A3.3,one type 32-A3.1,three type 32-B1.1,one type 32-B2.1, and two type 32-B3.I.The patients were treated with closed or indirect reduction and fixation with percutaneous LISS plate reversely.Results There were no major postoperative complications in this study.The mean operation time was 65 minutes (range,50 to 90 minutes),the mean intra-operative blood loss was 142 milliliters (range,50 to 400 milliliters),and the mean postoperative hospital stay was 9.3 days (range,6 to 15 days).All patients had a clinical follow-up;the mean follow-up time was 7.2 months (range,3 to 14 months).Ten cases healed clinically three months postoperatively,one periprosthetic fracture healed four months and one pathological fracture healed six months after operation.At the final radiographic follow-up,no collapse,varus deformity,cutting-out,hardware failure,or avascular necrosis was found.Conclusions Femoral LISS used reversely can provide secure fixation for proximal femoral fractures biomechanically and anatomically.It is also easy and safe.It is particularly suitable for old patients with intertrochanteric fractures complicated with disorders of internal organs and severe osteoporosis or patients with complex proximal femoral fractures.It is important to be skillful in indirect reduction and positioning of guide wire into hole A,and to avoid immediate weight-bearing postoperatively.

Objective To comprehend the awareness rate and the implementation of risk factors related to Helicobacter pylori (HP) infection in families with children of Helicobacter pylori infection.To analyze the existing problems and formulate effective methods of health education.Methods Totally 558 families infected with HP were investigated with a self-designed structured questionnaire by call-back.Results 527 did not use separate eating tools,350 did not use independent tablewares,14 drank unboiled water,14 did not use bath appliances separately,7 used tooth glasses together,4 did not wash hands before eating and after toilet,no one had mouth-to-mouth feeding.248 children with HP sleeped with their family members and 246 family members used to have HP infections or stomach troubles.Conclusions The families with HP do not establish correct ways of separate eating,so the hospital need to develop multi-channel education.Most families with HP infected children have satisfactory health habits.Nearly half of the children with HP do not sleep in their own beds.Caregivers of children with HP infection should be the capital objects of health education.

Objective To explore the relationship between the quantitative parameter in transrectal power Doppler ultrasonography (PDUS) and angiogenesis of prostate nodules in pathology. Methods PDUS and the analytic software of color flow were used, and peak vascularization indexs (PVIs) of 94 prostate nodules in 73 patients were recorded. The biopsy specimen was stained with CD34 antibody, and the microvessel density (MVD) was calculated. The correlation between PVI and MVD was statistically analyzed. Results PVI and MVD of the malignant nodules, in both transition zone (TZ) and peripheral zone (PZ), were significantly higher than those of the benign nodules, respectively (P＜0.05). There was positive correlation between PVI and MVD of the malignant nodules (rTZ=0.76;rPZ=0.79), but no correlation between PVI and MVD was found in TZ of benign nodules (r=0.18), while there was correlation between PVI and MVD in PZ (r=0.44). In TZ, the sensitivity and speciality of differentiation malignant nodules from benign nodules were 78.57% and 80.77%, respectively, and in PZ they were 62.07% and 91.00%, respectively. Conclusion As a quantitative parameter in PDUS, PVI can indirectly reflect the angiogenesis of prostate cancerous nodules, providing a new method for differentiation malignant nodules from benign nodules.

BACKGROUND: Pioglitazone is a common hypoglycemic drug capable of improving proliferation and activation, and inhibiting apoptosis of endothelial progenitor cells (EPCs). We speculated that the combined use of pioglitazone and EPCs transplantation could have significant improving effects on hyperglycemia and kidney function after diabetes mellitus.OBJECTIVE: To investigate the improving effect of EPCs transplantation combined with pioglitazone treatment on the kidney function of diabetic rat models.METHODS: The 15 of 75 Wistar rats were randomly selected and served as normal control group (no treatment). Animal models of type 1 diabetes mellitus were made in the rest 60 rats through the intraperitoneal injection of 40 mg/kg streptozotocin for continuous 5 days. The human EPCs (labeled by CM-Dil) were recovered, cultured and preserved until transplantation. After 4 weeks of modeling, the 60 rat models were randomly divided into model group (PBS injection), pioglitazone group, EPCs transplantation group and combined treatment group, followed by tail vein injection of EPCs suspension and/or intragastric administration of 20 mg/kg pioglitazone for continuous 4 days. After 8 weeks of treatment, the levels of glucose, insulin and creatinine in serum, urea nitrogen and urine protein during 24 hours were determined. The number and distribution of EPCs labeled by CM-Dil were detected by fluorescence microscope, the apoptosis in kidney cells was tested by TUNEL method, and the kidney weight/body weight ratio in rats was calculated.RESULTS AND CONCLUSION: Compared with the model group, the blood glucose and serum creatinine levels and the urea nitrogen and urine protein concentrations during 24 hours were significantly decreased (P < 0.05), and the seruminsulin level was significantly increased (P < 0.05) in the pioglitazone and EPCs transplantation groups. These biochemical indexes in the combined treatment group were more significantly altered compared with the model group (P< 0.01). The kidney weight to the body weight ratio was lowest in the combined treatment group and lower in the pioglitazone and EPCs transplantation groups followed by the model group (P < 0.05). The order of apoptotic kidney cells labeled by TUNEL was as follows: model group > pioglitazone group > EPCs transplantation group > combined treatment group (P < 0.05). To conclude, the EPCs transplantation combined with pioglitazone treatment can decrease the blood glucose and serum creatinine levels and urea nitrogen and urine protein concentrations, improve the serum insulin level, reduce cell apoptosis in the kidney, and remit the kidney dysfunction of diabetic rats to a certain extent.