Objective To explore ductus venous abnormal -looking blood flow parameters and spectrum morphology in the application value of fetal congenital heart disease screening.Methods 210 cases of normal fetal antenatal examination were selected as a normal control group,48 cases of congenital heart disease the fetus,then the component type according to right heart function,to the abnormal ductus venous blood flow spectrum combined with pathologic contrast analysis.Results 48 cases of CHD in the fetus,18 cases of left heart system dysplasia,30 cases of right heart system dysplasia.In order to right heart function change respectively:right heart preload increased 15 cases,right heart load increased after 25 cases,8 cases of right heart function change was not obvious.Conclusion Ductus venous and parameters have abnormal blood flow spectrum,highly suggestive fetal congenital heart disease, especially dysplasia fetal heart right system can serve as an indicators of early screening for CHD.

Objective To discuss the effect of targeted information support on the sense of uncertainty and anxiety among patients treated with percutaneous coronary intervention and its instructive significance to nursing work.Methods We randomized 118 patients treated with percutaneous coronary intervention whose sense of uncertainty and anxiety were relative intensive into the test group and the control group with 59 cases in each group.We gave routine health instructions to the control group while targeted information support besides routine health instructions to the test group.We investigated the patients in two groups by Chinese version of uncertainty scale and State Anxiety Inventory at 4 time points,that is ,the first day in hospital,night before the operation,24 hours after the operation and 1 day before leaving hospital.Results The anxiety level and sense of uncertainty about illness decreased at the night before operation,24 hours after operation and 1 day after operation compared with the control group.The sense of uncertainty and anxiety level has statistical difference between whatever two adjacent time points except for anxiety level between the time points between the first day in hospital and before operation.Drop of sense of uncertainty at 24 hours after operation was significantly different between the two groups(P<0.01).Conclusion Supplying targeted information support could decrease the sense of uncertainty and anxiety among patients treated with percutaneous coronary intervention.

Objective To investigate the subjective well-being and its influencing factors of the advanced elderly in the community. Methods Totally 409 cases aged 80 years and over in five communities of Hang zhou city were surveyed using Memorial University of Newfoundland Subjective Well-bing Scale. Results The subjective well-being of the advanced age people was at a high level.The cases with good hearing,well eyesight,income from own source,monthly income between 1000-3000 yuan,health satisfaction and frequently or occasionally regular interest in the activities had higher subjective well-being(all P＜0.05).Subjective well-being was not significantly affected by age,sex,level of education (P＞ 0.05). Conclusions Subjective well-being of aged people is mainly affected by state of health and economic resources.

Amino Acid Metabolism, Inborn Errors ; Humans ; Mitochondrial Diseases ; Sarcosine Dehydrogenase ; deficiency

Good palatability of pediatric drugs improves the accuracy of dosing and adherence to dosing regimens. Many active pharmaceutical ingredients have an undesirable taste, so effective taste masking methods need to be developed to improve the palatability of pediatric medicines. This article mainly introduces the commonly taste masking techniques and taste evaluation methods, and puts forward suggestions on the concerns and general principles of the pharmaceutical evaluation of taste masking effects based on cases, in order to provide reference for the development of drug taste masking technology in children.

Clinical Trials as Topic ; Dentistry ; Humans

Histone deacetylase 6 (HDAC6) is an unique subtype of histone deacetylases with two tandem deacetylase domains and substrate specificity for non-histone proteins. It is involved in many important physiological and pathological processes and has become a promising therapeutic target in recent decades. Different kinds of potent HDAC6-selective inhibitors have been reported around the world. This paper reviews the progress in the study of structure and functions of HDAC6 as well as the development of HDAC6-selective inhibitors.
Histone Deacetylase Inhibitors ; pharmacology ; Histone Deacetylases ; chemistry ; Humans ; Substrate Specificity

Objective To investigate epigenetic inactivation of SPOCK2 gene in the malignant transformation of ovarian endometriosis ( EM ) by comparing the methylation status and protein expression of SPOCK2 gene in the malignant tissues,ectopic endometria and the eutopic endometria of endometriosis.Methods From Jan.2005 to Jan.2011,22 paraffin-embedded specimens diagnosed as malignant transformation of ovarian endometriosis ( EAOC ) including 11 cases with ovarian endometrioid carcinoma,8 cases with clear cell carcinoma,2 cases with serous cystadenocarcinoma and 1 case with mucous cystoadenocarcinoma matched with 22 cases with ovarian endometriosis and 16 cases with normal endometrium form cervical intraepithelial neoplasia(CIN) patients as controls in Department of Obstetrics and Gynecology of Shengjing Hospital.Twenty-two malignant tissues,15 ectopic endometria and 10 eutopic endometria were captured by microdissection in EAOC group; 22 eetopic endometria and 17 eutopic endometria were captured in EM group; 22 endometrium were captured in the NE group.The methylation statue of SPOCK2 was determined by combined bisulfite restriction analysis,and the protein expression of SPOCK2 was evaluated by immunohistochemistry.Results ( 1 ) Methylation of SPOCK2:in the EAOC group,the frequency of SPOCK2 hypermethylation in malignant tissue was 45％ (10/22),which was significantly higher than 1/15 in the ectopic endometrium (P＜0.05).There was no statistical difference of the frequency of SPOCK2 hypermethylation in ectopic endometrium in the EAOC group(1/15) and EM group (5％,1/22) (P＞0.05).(2) SPOCK2 protein:the loss rate of SPOCK2 was 44％ (11/22) in malignant tissue in EAOC group,which were significantly higher than 2/15 of in ectopic endometrium of EAOC (P ＜ 0.05).However,there was no remarkable difference in loss rate of SPOCK2 protein between ectopic endeometrium of EAOC and endometrium of EM [ 2/15 vs.5％ ( 1/22 ),P ＞ 0.05 ].No significantly difference in loss rate of SPOCK2 in eutopic endometrium was observed among three groups ( P ＞ 0.05 ).(3) The abnormal methylation of SPOCK2 could lead to loss expression of protein ( P ＜ 0.05 ).Conclusion Epigenetic inactivation of SPOCK2 gene is involved in the malignant transformation of ovarian endometriosis.

Integrative Medicine ; Medicine, Chinese Traditional

Objective:To establish a method for the simultaneous determination of fluoxertine and olanzapine in capsules by re-versed-phase high performance liquid chromatography ( RP-HPLC) . Methods:The successful separation of fluoxertine and olanzapine was achieved on a Phenomenex C18 column (250 mm × 4. 6 mm, 5 μm) with 10 mmol·L-1 potassium dihydrogen phosphate buffer (pH 4. 0)-acetonitrile-methanol (55 ∶40 ∶5, v/v/v) as the mobile phase. The flow rate was 1. 0 ml·min-1, the detection wave-length was 227 nm, the column temperature was 30℃, and the injection volume was 20 μl. Results:Fluoxertine could be well separa-ted from olanzapine under the chromatographic conditions. The linearity between the peak areas and the concentrations was observed within the range of 5. 0-80. 0 mg·L-1(r=0. 999 9) for fluoxertine and 1. 2-19. 2 mg·L-1(r=0. 999 9) for olanzapine. The mean recovery of fluoxertine and olanzapine was 99. 9%(RSD=0. 94%, n=9) and 100. 2%(RSD=2. 08%, n=9), respectively. Con-clusion:The method is simple, sensitive and accurate, and it can be applied in the content determination of fluoxertine and olanzapine in capsules.