OBJECTIVE:To study the combined antibacterial activity of Rhizoma Coptidis,Bee Propolispro and Fructuts Evodiae against Helicobacter pylori(Hp).METHODS:The protocol was designed by checkboard method.The MICs of Rhizoma Coptidis,Bee Propolispro and Fructuts Evodiae alone or in two-and-two combination against Hp were determined by agar plate method,and the FIC indexes was calculated for the evaluation of the combined effects.RESULTS:When Rhizoma Coptidis combined with Bee Propolispro,the FIC index was 0.16(0.5)when combined with Fructuts Evodiae;The FIC index was 0.05(

OBJECTIVE:To select the best medium prescription of HP.METHOD:To select the best solid medium of HP by orthogonal test design and to study the condition of preserving HP.RESULTS & CONCLUSIONS:There are significant deviations of growing circumstance of HP on the different cultivation medium.The best solid cultivation medium for HP is colombian agar or brain heart infusion agar medium with 2.5%mixed antibiotic added with 5.0%bovine serum and kept the pH of medium at 7.5.HP is preserved under —70 ℃～—80 ℃,which could be successfully antibiotic.

OBJECTIVE: To construct hospital pharmaceutical website and facilitate the evolvement of hospital pharmaceutical care.METHODS: Website management system was developed independently and the system was used to construct hospital pharmaceutical website in our hospital.RESULTS & CONCLUSIONS: Website management system which was designed with B/S three-tier structure which sustains multi-level management authority control and a variety of database and runs stably.The professional website based on website management system is convininent and maintenance,rapid in operation,cheap in cost,low in consumption of human resouces,comprehensive in function,highly professional,and it has greated enhanced the working efficiency in hospital pharmacy.

Magnetic resonance imaging (MRI)has apparent dominance in nasopharyngeal carcinoma (NPC) diagnosis,staging,the plan of radiotherapy target and assessment of efficacy because of its high-contrast of parenchyma and muli-parameters imaging.Especially diffusion-weighted imaging (DWI) developed in several years,it can provide qualitative and quantitative information at the cellular level about tumor cellularity and the integrity of cell membranes,which is valuable for tumor detection,cervical lymph nodes metastases,systemic transfer,monitoring and predicting the treatment response of nasopharyngeal carcinoma.

[Objective]This paper will pitch-in TCM psychosomatic theory(from Huangdi Neijing),exploring the disorders of depression with the metabolic syndrome and pathogenesis of traditional Chinese medicine,so as to provide effective ideas for the treatment of depression with metabolic syndrome.[Methods]:Through expansion on traditional chinese medicine's basic understanding of depression with metabolic syndrome,utilizing basic TCM viscera-state theory, from the causes of this disease,key factors,pathogenesis transformation three aspects to discuss the pathogenesis of the disease. [Results]By discussing the heart,liver,spleen function of zang-fu organs and their mutual relations in the role and impact of the disease development process, this paper thinks of the nature of disease as asthenia in origin and asthenia in superficiality,which locates in spleen,has association with heart and liver, phlegm, qi and blood stasis is the disease of pathological product of pathological factors to promote the development of the disease.Kidney would be involved in it with long course of disease.The therapy could focus on tonifying spleen,nourishing heart and soothing liver,with methods of promoting the circulation of qi,removing blood stasis and reducing phlegm,meanwhile looking out to protect kidney.[Conclusion]Disorder of depression and pathogenesis of TCM of metabolic syndrome,to guide the clinical treatment of the disease and the theory of traditional Chinese medicine development has practical significance.

Small intestinal submucosa has been applied in a variety of areas of basic and clinical study with its Characteristics of anti-infective and Low immunogenicity and so on.However,it is common to find the shrinkage and other problems in some tissue repair,and affects the functional recovery of repaired tissue to a certain extent.Thus,it is still a question to be resolved that how to avoid the shrinkage and other adverse effects after tissue repair.This paper provides a reliable basis for further correct understanding of the SIS's capacity of tissue repair by reviewing and summarying its characteristics and present situation of tissue repair,especially put forwarding the shrinkage problem.

OBJECTIVE:To probe into the characteristics and general pattern of new ADR in our hospital. METHODS:A to-tal of 151 new ADR cases reported to Nationwide ADR Monitoring Network by our hospital during 2013 to 2014 were analyzed statistically. RESULTS:Of the 151 ADR cases,males(56.29%)showed higher proportion than females(43.71%);most of ADR cases were induced by anti-infective agents,including 45 cases(29.80%). ADR cases most easily induced by injection and intrave-nous dripping,accounting for 83.44% and 91.39 %,respectively. Lesions of skin and its appendants were most common clinical manifestations of ADR,which accounted for 28.82%(66 cases). There were 10 new and severe ADR cases(6.62%). All of ADR cases were cured or improved. CONCLUSIONS:More attention should be paid to ADR monitoring especially indication of anti-in-fective agent to reduce the occurrence of ADR.

Objective To investigate the changes and significance of serum N-terminal pro-brain natriuretic peptide ( NT-proBNP) levels in mice model with viral myocarditis( VMC) . Methods 100 Balb/c mice were randomly di-vided into VMC group ( n=60 ) and control group ( n=40 ) . Mice in VMC group were injected intraperitoneally coxsackievirus B3 ( CVB3 ) , while mice in control group were treated with the same amount of virus-free DMEM cul-tivate liquid. 10 mice of each group were sacrificed on 3, 7, 14, and 28th day after VMC modeling and their blood and heart tissues were collected. The serum NT-proBNP levels in two groups of mice were detected by ELISA. Pathological changes in myocardial tissue were examined with hematoxylin and eosin stain. The relationship be-tween serum NT-proBNP concentration and cardiac pathology score of VMC group at different time points were ana-lyzed. Results The serum NT-proBNP levels of VMC group were significantly higher than that of control group( P<0. 05 ) . There was a significant statistical difference between NT-proBNP levels of VMC group at different time points( P <0. 05 ) . The NT-proBNP level was positively correlated with myocardial histopathology scores ( r =0. 829 , P<0. 05 ) . Conclusion Serum NT-proBNP levels may reflect severity of myocardial injury in mice with VMC. NT-proBNP can be used as one of serum indicators to judge the risk stratification in mice with VMC.

Objective To translate the English version of Diabetes Continuity of Care Scale (DCCS) into Chinese and to test the reliability and validity of the Chinese version of DCCS in diabetes mellitus patients.Methods Totally 245 patients with diabetes mellitus were recruited and were investigated by the Chinese version of DCCS.Results The internal consistency coefficient of the Chinese version of DCCS ranged from 0.728 to 0.942,which was ＞0.70;split half coefficient r=0.784,and the test-retest was 0.864 while which of each dimension ranged from 0.785 to 0.886.The content validity index was 0.934.Factor analysis got five factors,which explained 76.574％ of the total variance;the factor loading of each item was ＞0.4.Conclusions The Chinese version of DCCS has been proved to be reliable and valid.It can be used as a valid tool for the measurement of continuity of care in patients with diabetes mellitus.

Non-cirrhotic portal hypertension(NCPH)is a group of diseases that show evidences of portal hypertension but no cirrhosis is present.Common causes of NCPH include pre-sinusoidal portal lesions such as portal vein thrombosis,congenital liver fibrosis and idiopathic portal hypertension,and post-sinusoidal portal lesions.The major feature of this group of diseases is well preserved liver function in spite of prominent portal hypertensive manifestations such as esophageal varices/gastrointestinal bleeding and splenomegaly/hypersplenism.Careful differentiation from cirrhosis requires thorough clinical,radiological and pathological investigation.Preventing and control of variceal bleeding and hypersplenism through medical,endoscopic and interventional procedures yield good prognosis in most of the patients with NCPH.