OBJECTIVE: To study on the effect of different extracting methods on the content determination of polysaccharide from Caulis Dendrobii.METHODS: Polysaccharide was extracted from Caulis Dendrobii respectively by reflux extraction,improved reflux extraction,ultrasound wave extraction and ultrasonic-assisted thermal reflux extraction.The content of polysaccharide was determined by phenol-sulfuric acid method.RESULTS: The determined results using these methods were as follows:(13.473?0.634)%,(15.692?0.743)%,(11.87?0.536)% and (16.29?0.407)%.CONCLUSION: The content determination of polysaccharide from Caulis Dendrobii was associated with the extraction method.The polysaccharide from Caulis Dendrobii could be extracted more thoroughly and rapidly by ultrasonic-assisted extraction technology.Therefore this method could be used to pretreat the sample in content determination of Caulis Dendrobili polysaccharide.

Objective To investigate the relationship of serum uric acid(SUA) with type 2 diabetes mellitus and diabetic macrovascular complications and to study the influential factors of SUA.Methods Recruited were eighty type 2 diabetic patients(group B),ninety-two type 2 diabetic patients with coronary artery disease(group C) and one hundred and twelve healthy controls(group A) whose qualitative urine protein assay were negative.Serum concentrations of uric acid and other biochemical materials were assayed at a fasting state.The sensitivity of insulin was also evaluated.Results(1) The SUA levels in group A,B and C were(280±81)(257±76) and (302±72) mmol/L respectively;SUA in group B was lower than that in group A(P=0.037) or group C(P=0.000) and SUA in group C tended to be higher than that in group A(P=0.050).(2) SUA level significantly increased in man than in woman(P=0.001).(3) The independent influential factors of SUA were WHR(R=0.354) in man,and BMI(R=0.286) and age(R=0.623) in woman.(4) After adjusting the age,sex,distribution of body fat and serum lipid level, the logistic regression analysis of group B and C showed that hyperuricemia was the independent risk factor for diabetic macrovascular complications,and this was especially obvious in smoker or hypertensive patient.Conclusions SUA in type 2 diabetic patients is lower than that in both healthy controls and type 2 diabetic patients with coronary disease.The level and contributing factors of SUA are different between men and women.

Objective To observe and study the application and effect of antimicrobial agents in type Ⅰ general surgery, in order to rationalize the use of drugs.Methods 228 patients with type Ⅰgeneral surgery from May 2014 to September 2016 were selected as the observation group.The patients in the type Ⅰ general surgery group from February 2012 to April 2014 were selected as the control group.The observation group were retrospectively monitored, and the control group were prospectively monitored.The application of antimicrobial agents in the two groups of patients, the rational application and the application rate of antimicrobial agents in type Ⅰ general surgery and outcome were compared.Results The application rate of the first generation cephalosporins in the observation group was significantly higher than that in the control group ( P<0.05 ).There was no significant difference in application rate of macrolides compared with the control group.The application rates of the second generation and third generation cephalosporins, quinolones, aminoglycosides and nitroimidazoles were lower than those of the control group ( P<0.05 ).And the rational use of antimicrobial agents, reasonable choice of time, rational combination of drugs, reasonable dose, reasonable frequency of administration and reasonable volume of solvent were higher than those of the control group, the differences were statistically significant (P<0.05).The antibiotic application rates of abdominal hernia surgery, thyroid surgery and breast surgery were lower than those of the control group, the differences were statistically significant (P<0.05).There was no infective case in the two groups.And the average length of stay in the observation group was (8.50 ±1.20) days, which was lower than (15.00 ±2.30) days in the control group, the difference was statistically significant (P<0.05).Conclusion Accordance with the principles of antibiotics in type Ⅰ general surgery, it could rationalize medication and reduce the drug resistance.

The design principles of healthcare wearable devices: ambient intelligence, service continuity, and micro-context provide more choice and solutions for the healthcare and social needs, and have the potential to be an integral part of the modern health care system. The use of wearable devices will contribute to the innovation of healthcare data acquisition and healthcare behaviors, the promotion of health consciousness and literacy, it also benefits the patient education, clinical pathway, medical model and health management performance.

Female ; Hepatitis C ; complications ; drug therapy ; Humans ; Interferon-alpha ; therapeutic use ; Interferons ; therapeutic use ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive ; complications ; drug therapy ; virology ; Middle Aged ; Polyethylene Glycols ; therapeutic use ; Recombinant Proteins ; therapeutic use ; Treatment Outcome

Objective To observe the clinical efficacy of acupuncture at the six mind-calming points plus the four gate points in treating migraine.Method Totally 128 eligible migraine patients were randomized into a treatment group and a control group, 64 cases in each group. The treatment group was intervened by acupuncture at the six mind-calming points plus the four gate points, while the control group was by ordinary acupuncture. The Numerical Rating Scale (NRS), headache attack frequency, headache duration, headache intensity, complicated symptoms, Self-rating Depression Scale (SDS), and Self-rating Anxiety Scale (SAS) were observed before and after treatment and in the follow-up study.Result The NRS scores and the headache symptoms and functions scores were significantly changed respectively after 2-week and 4-week treatment in both groups (P<0.01,P<0.05). The NRS score, headache duration, and headache intensity in the treatment group were significantly different from that in the control group after 4-week treatment and in the follow-up study (P<0.05,P<0.01). The SDS and SAS scores were significantly changed after 2-week and 4-week treatments as well as in the follow-up study in the treatment group (P<0.01). The SDS and SAS scores were significantly changed after 4-week treatment in the control group (P<0.01). There were significant differences in comparing the SDS and SAS scores between the two groups after 2-week and 4-week treatment as well as in the follow-up study (P<0.05,P<0.01). In the treatment group, the SDS and SAS scores in the follow-up study were significantly different from that after 2-week treatment (P<0.01).Conclusion Acupuncture at the six mind-calming points plus the four gate points is an effective method in treating migraine, and it can reduce headache intensity, and improve the anxiety and depression state.

Objective　To establish the method of 2-dimensional electrophoresis(2-DE) for proteins of human spermatozoa and to construct a protein map of human spermatozoa. Methods　The sperm pellet was prepared with simple Percoll layer protocol. We studied the effects of various sample preparation methods, loading quantities and isoelectric-focusing protocols on the quality of silver-stained 2-DE map, and constructed a primary protein map of human spermatozoa. Result　Up to 703 protein spots were acquired with sample preparation Method Ⅰwhile only 194～210 spots with Method Ⅱ.With immobilized pH gradients and sodium dodecyl sulfate-polyacrylamide gel electrophoresis(IPG-DALT) we could acquire over 700 spots while only 280～300 with isoelectric focusing and sodium dodecyl sulfate-polyacrylamide gel electrophoresis(ISO-DALT). Conclusion　It is satisfactory to lyse sperm with sample preparation Method Ⅰ and to separate sperm proteins by IPG-DALT for establishing 2-D map of human sperm.

Objective To observe the effects of general anesthesia and combined spinal epidural anesthesia in elderly patients with lower limb fracture surgery, and to evaluate the effect of postoperative cognitive function and pain score.Methods Retrospective analysis, screening of intraoperative anesthesia, postoperative change lost invalid cases, from November 2012 to October 2015 in our hospital accepted surgical treatment of 180 patients with lower limb fracture surgery were selected according to different patients with different anesthesia methods can be divided into treatment group and control group, 90 cases each group of patients.Patients in the treatment group were treated with combined spinal epidural anesthesia , while the control group received general anesthesia.The incidence of cognitive dysfunction and postoperative pain scores were compared between the two groups. Results the incidence of cognitive dysfunction after surgery in 90 cases of observation group was lower than that of the control group , and the difference was statistically significant (P<0.05).The evaluation of two groups of postoperative chronic pain for 3 days, 6 days, 10 days, 15 days, the incidence rate of VAS score and the character, whether the impact on the daily life and the rest, the patients in the treatment group pain scores were significantly lower than the control group, the difference was significant (P <0.05).Conclusion After lumbar epidural anesthesia in elderly lower limb fracture surgery effect on cognitive function in patients with small, and can reduce the pain of patients, in the actual work according to the actual situation of different specific problems in different specific analysis.

Objective To investigate the correlation between the HSP90 expression and invasion and metastasis potential of human breast cancer cell line. Methods The mRNA expression level of HSP90 was detected by RT-PCR. The protein expression level of HSP90 was measured by Western blotting. The effects of HSP90 inhibitor Geldanamycin (GA) on the invasion and motility abilities of human breast cancer line MDA-MB-231 and MDA-MB-435s in vitro were explored by transwell chamber. Results The mRNA expression level of HSP90? in MDA-MB-435s cell was higher than that of MDA-MB-231 cell (P0.05) between the two cell lines. The protein expression level of HSP90 in MDA-MB-435s cell was higher than that of MDA-MB-231 cell (P0.05). After treated with GA, the invasive and metastatic potential in human breast cancer cell MDA-MB-435s and MDA-MB-231 was suppressed significantly (P

GRP78,as endoplasmic reticulum resident chaperone,is highly induced by a variety of tumor microenvironmental stresses,such as hypoxia and glucose deprivation.GRP78 is implicated in tumor cell proliferation,apoptosis,invasion,metastasis and angiogenesis.Recent evidence indicates that GRP78 levels is correlated with pathological grade,stage and prognosis for the majority of solid tumors.In addition,GRP78 plays an important role in drug tolerance and knockdown of GRP78 has been shown to sensitize malignant cells.GRP78 could not only be a good biomarker to predict cancer progression and chemo-responsiveness,but also an appealing target for the development of a more selective chemotherapy.