Adaptive radiotherapy (ART) is the product of excellence technology.It reschedules,registers according to tumor's size,shape and alteration of relative position with the surrounding tissue during the treatment.Thereby it raises the accuracy of the conformal radiotherapy.The technology is gradually being chosen by more and more patients.The technology can increase the rate of local control significantly,while reducing the occurrence of radiation-related adverse reactions.

Six patients with lung cancer treated with therapy were included. They were retrieved the image and re-planned by fraction 10. The volume of GTV was reduced (65. 59 ± 17. 09)% after three-stage treatments. The doses of target tumor were the same in three-stage treatments. Compared with IMRT, ART1 reduced V20 , V30 , MLD of whole lung by 2. 01%,2. 66%,241. 78 cGy(P=0. 005,0. 039,0. 026). Compared with ART1,ART2 further re-duced the indexes by 3. 21%,2. 97%,288. 64 cGy(P=0. 005,0. 013,0. 046). The doses of ipsilateral lung were reduced significantly (P<0. 05). But this was not for opposite lung. The dose V50,Dmean of heart and V55 of esopha-gus were similar to each plan. Dmean of esophagus with ART1 was smaller than with IMRT(261. 98 cGy,P=0. 002) and Dmean of esophagus with ART2 was smaller than with ART1(300. 43 cGy,P=0. 008). D2% and Dmean of spinal cord were reduced in turn significantly(P<0. 05).

Objective To investigate the effect of membrane-cytoskelet on linker Ezrin in the invasion and migration of human adenocarcinoma cells line AGS. Methods Two small hair RNA targeting Villin2 gene ( Ezrin coding gene) was designed,chemically synthesized and inserted into plasmid pGenesil-shRNA,then transformed into E. coli DH5 ?. The recombinant plasmid was extracted in middle quantity and transfected into AGS cells by LipofectamineTM 2000. Ezrin expression in AGS cells transfected by shRNA recombinant plasmid was measured by RT-PCR and Western blotting. AGS cells were transfected by effective shRNA plasmid and cultured in 1640 media containing G418 ( 800 ?g/ml) . RT-PCR and Western blotting were applied respectively to detect the Ezrin mRNA and protein expression to evaluate the blocking effect of shRNA-Ezrin. Cell migration and invasion was determined by scratch test and Transwell chamber assay. Results Our designed shRNA knocked down 93% Ezrin in AGS cells which had a stable expression of Ezrin small hairpin RNAs. The migrated cells number of Ezrin knocked-down group was 27. 67 ? 4. 50,which was significantly decreased compared with the control group ( 125. 50 ? 8. 33,P

Objective To conclude the treatment of femoral shaft fractures with ipsilateral femoral neck or intertrochanteric fractures by dynamic condlar screw(DCS) and proximal femoral nail(PFN).Methods From January 1998 to June 2005,35 patients of femoral shaft fractures were studied.10 patients were associated with ipsilateral femoral neck fractures,25 patients with ipsilateral intertrochanteric fractures.9 cases were treated with DCS.26 cases were treated with PFN.Results The follow-up ranged from 6 to 30 months with an average of 18 months.All the fractures healed up well except that 2 cases happened with ischemic femoral necrosis.The rate fractures healing was 94%.Conclusion DCS is an ideal method to treat stable fractures.PFN is excellent in less invasion and shorten operation time.DCS and PFN have obvious advantages of reliable fixation short,clinical healing time and post-operative restored function.

Objective: To examine the reliability and validity of the Chinese version of the Friendship Quality Inventory(FQI). Methods:1166 undergraduates were administered the Chinese version of FQI. Using EFA and CFA, a four-factor model was tested. The internal reliability, test-retest reliability and item-analysis were also examined. Results:EFA showed that undergraduates' friendship quality was composed of assistance and support, friends conflicts, companionship and intimacy. The NFI(0.993)、RFI(0.990)、TLI(0.995)、IFI(0.992)、CFI(0.995)、RMSEA(0.051)all met the criteria for adequacy of fit in CFA. The Cronbach's a coefficient of FQI was 0.754 and the four dimensions was 0.585～0.713; The test-retest reliability coefficient was 0.792; The correlation coefficient of item-between-item in the same dimension was 0.184～0.470. Conclusion:The Chinese version of FQI has good reliability, validity as well as discrimination .

Genome replication of reovirus occurs in cytoplasmic inclusion bodies called viral factories or viroplasms. The viral nonstructural protein μNS, encoded by genome segment M3, is not a component of mature virions, but is expressed to high levels in infected cells and is concentrated in the infected cell factory matrix. Recent studies have demonstrated that μNS plays a central role in forming the matrix of these structures, as well as in recruiting other components to them for putative roles in genome replication and particle assembly.

The clinical symptoms of functional constipation in the elderly are more unique which represents as over concern about their bowel movement,excessive straining and worse feelings to the constipation.The intestinal physiological function declining with age,reductions of enteric neurons and interstitial cells of Cajal,neuronal degeneration and injured contractibility of intestinal smooth cells are enrolled in the pathogenesis of functional constipation.More attention should be paid to drug interactions and adverse effects in clinical practice due to more underlying disease and concomitant medication in the elderly.

Objective To evaluate the feasibility and techniques of hand-assisted laparoscopic resection using the LapDisc in patients with hepatic hemangioma. Methods A total of 8 patients with hepatic hemangioma underwent hand-assisted laparoscopic partial hepatectomy. The patients consisted of 6 men and 2 women, with the age ranging 30~72 years (mean, 47.6 years). Results The hand-assisted laparoscopic hepatectomy was successfully performed in all the patients. The average operating time was 196.3 min (range, 110～350 min), the average blood loss was 307.5 ml (range,100～750 ml), and the average postoperative hospital stay, 7.9 days (range,6～15 days). No postoperative complications such as bile leakage, intraabdominal bleeding or infection occurred. Conclusions Hand-assisted laparoscopic hepatectomy using the LapDisc is safe and feasible for patients with hepatic hemangioma with surgical indications.

Objective To investigate clinical effects of hand-assisted laparoscopic radical resection for colorectal cancers. Methods A series of 27 cases of colorectal cancer received hand-assisted laparoscopic radical surgery using the LapDisc. Results All the operations were successfully accomplished without conversions to open surgery. The operation time was 90~260 min (mean, 140 min), and the intraoperative blood loss was 50~200 ml (mean, 110 ml). No deaths or anastomotic leakages were observed after surgery. Follow-up for 6~23 months (mean, 8.6 months) found no port-site metastasis. Conclusions Hand-assisted laparoscopic radical resection for colorectal cancers gives advantages of safety, minimal invasion, quick recovery, and simplicity of performance.

0.05).Compared with control group,contents of sialic acid in the high and moderate dose groups,epididymal coefficient and contents of carnitine in the high dose group reduced significantly(P