OBJECTIVE To investigate the distribution,epidemic trend and transition of drugs′ sensitivity about the pathogenic bacteria of children′s urinary tract infection(UTI) in the past 8 years in the local area.METHODS The medical records of 391 UTI cases which were hospitalized in our hospital and confirmed as UTI through urine culture from 2000 to 2007,were reviewed and the clinical features were analyzed.RESULTS A total of 402 pathogenic bacteria were determined.Gram-negative bacilli were predominant,accounted for 72.9%,and Escherichia were the most commonly found(34.4%).Gram-positive cocci accounted for 27.1%,including 12.9% of Staphylococcus and 10.4% of Enterococcus.The proportion of Escherichia,Klebsiella and Enterococcus was increased year by year.Gram-negative bacilli had a high resistance rate to ampicillin and 1st to 4th generation cefalosporins and a low resistance rate to piperacillin/tazobactam.Gram-positive cocci had a high resistance rate to ampicillin and erythromycin and a low resistance rate to nitrofurantoin and vancomycin.In the past 8 years,the resistance rate to most of the antibacterial agents has been increased,especially ampicillin and cephalosporins.Only piperacillin/tazobactam and vancomycin maintained high antibacterial activities.CONCLUSIONS Gram-negative bacilli are the major pathogens in pediatric UTI,and Gram-positive cocci appear to be increasing.Ampicillin should not to be chosen commonly.The observation of variation of pathogenic bacteria and drug resistance is of great importance.

OBJECTIVE:To observe the effectiveness and safety of Jin's three-needle combined with Jiawei wumei pill in the treatment of menopausal insomnia.METHODS:One hundred and sixty-five patients with perimenopausal insomnia were selected from our hospital during Jan.2015-Mar.2016,and then divided into group A (n=41),group B (n=41),group C (n=41) and group D (n=42) according to random number table.Group A was given Estazolam tablet 1 mg,qd;group B was given Jin's three-needle 30 min/time,qd;group C was given Jiawei wumei pill,one pill,after breakfast and supper warm taking;group D was given Jin's three-needle combined with Jiawei wumei pill with same usage as group C.Four groups were treated for 4 weeks.PSQI score,modified Kupperman score,life quality score,serum levels of E2 and FSH were observed in 4 groups before and after treatment,and the occurrence of ADR was recorded.RESULTS:Before treatment,there was no statistical significance in above score (P＞0.05).After treatment,PSQI score and modified Kupperman score of 4 groups were decreased significantly,compared to before treatment;the group B,C,D were significantly lower than the group A;the group D was significantly lower the group B,C,with statistical significance (P＜0.05).The life quality score of 4 groups were increased significantly,and the group B,C,D were significantly higher than the group A;the group D was significantly higher than the group B,C,with statistical signifi cance (P＜0.05).E2 level of group B,C,D were significantly increased,while FSH level was decreased significantly,the improvement of group D was significantly better than group B,C;the improvement of group D was significantly better than that of group B,C,with statistical significance (P＜0.05).The incidence of ADR in group A was significantly higher than group B,C,D,with statistical significance (P＜0.01),and there was no statistical significance in the incidence of ADR among group B,C,D,with statistical significance (P＞0.05).CONCLUSIONS:Jin's three needle combined with Jiawei wumei pill is effective in the treatment of perimenopausal insomnia with good safety.

Administration, Inhalation ; Endothelium-Dependent Relaxing Factors ; pharmacology ; Humans ; Hypertension, Pulmonary ; drug therapy ; Nitric Oxide Donors ; administration & dosage ; therapeutic use ; Nitroglycerin ; administration & dosage ; therapeutic use ; Nitroprusside ; administration & dosage ; therapeutic use ; Phosphodiesterase Inhibitors ; administration & dosage ; therapeutic use

Objective To investigate the effect of thiopental on lipopolysaccharide(LPS)-induced activation of nuclear factor kappa B(NF-κB) under different concentration or after different time in rat glioma cells in vitro.Methods The rat C 6 glioma cells were treated with,in the presence or Absence of various concentrations(10-3 mol/L,10-4 mol/L and 10-5 mol/L)of thiopental for 1 h.30 min before harvest,the cells were exposed to LPS(10 ng/ml).The other C 6 cells were treated with 10-5 mol/L thiopental with different time(0.5 h,1 h,2 h,4 h).30 min before harvest,the cells were exposed to LPS(10 ng/ml).Nuclear extracts from C 6 cells were harvested using standard procedures,and the LPS-induced NF-κB expression was determined with electrophoretic mobility shift assays(EMSA) and measured with densitometry(Bio-Rad).Results The width of the bands of NF-κB under thiopental with different concentrations were similar,but they were narrower than that of the control(treated only with LPS),the density was less than that of the control(P<0.05).The width of the bands of NF-κB under thiopental with different time decreased with the time(P<0.05),and they were much narrower and shallower than that of the control(P<0.05),and the density was significantly less than that of the control(P<0.05).Conclusion Thiopental suppressed the activation of transcription factor NF-κB,and the inhibition was concentration-independent but time-dependent.

Objective To study the effect of selection of indications in percutaneous laser disc decompression(PLDD).Methods Lumbar disc herniation treated by PLDD with satisfactory and unsuited indications in 34 cases respectively were matched studied.Results The curative effect was 85.3% and 55.9% in satisfactory indication group and unsuited indication group respectively (?~2=5.06,P

BACKGROUND: In recent years, percutaneous laser disc decompression (PLDD) has been developed gradually in China. But there have been fewer further studies on the indications and contraindications for the use of the therapy.OBJECTIVE: To analyze the therapeutic effect after the choice of the indications for PLDD.DESIGN: A case-paired observational analysis with the patients as the subjects.SETTING: The Department of Radiology and Department of Soft Injuries of the 474 Hospital of Chinese PLA.PARTICIPANTS: We recruited inpatients of the 474 Hospital of Chinese PLA after PLDD between August 1998 and December 2004 as subjects for this research. Totally 68 cases were involved. According to the same gender, the closeness in age, the cases were matched and divided into 2 groups (eg. If there were 2 or more than 2 cases with the same age, the case whose disease course is the closest one was chosen): poor indication group (n=34) and good indication group (n=34).METHODS: A pillow was put under the belly of the patient who lay face down on the angiographic bed with the side with serious symptoms near the outside of the bed (the side where doctor operated). Symbols were marked on the body surface under the fluoroscopy. The waist was subjected to conventional sterilization and was paved with a piece of sterile cloth. 5 Ml of lydocaine was used for anesthesia. 8-12 cm to the posterior central midline of the diseased side, an incision was made to insert the needle according to the fat and thinness of the patients, and attention to the angle adjustment was needed to enter the intervertebral disc through "the secure triangle"beneath the nerve root so that 5 mm of the exposed front segment covered the needle end completely. Cautery was made with 15 W laser for every 1s after an interval of 4 s. The total output power of laser was adjusted according to the area of intervertebral disc, which was usually 1 200-1 700joules. The gas produced should be drawn out during the cautery, and this was done at least for 3 times. If the patient felt the distending pain, drawing out gas should be performed at any time. The puncturing needle and fiberoptics were pulled out after the operation, and band-aid was applied to the local area. The patients lay at the supine position to let the doctor examine the remission and functional recovery. When the patients were discharged, modified Macnab standard was used to evaluate the therapeutic effect. Evaluation criteria: According to the modified Macnab standard, the therapeutic effect can be classified into the following types: excellent means disappearance of pain, no limitation in motor function, with complete recovery of normal working and movement; good means that the patients showed occasional pain, but were able to do light job; passable means that the patients showed partial improvement, but still felt pain, and were not able to work and move; poor means that the patients showed signs of nerve compression that further operation was needed. The therapeutic effect of being excellent and good was combined as good therapeutic effect,and that of being passable and poor was combined as poor therapeutic effect. X2test was performed to the paired numerative data by the author.MAIN OUTCOME MEASURES: ① General evaluation of the therapeutic effect of the poor indication group and good indication group according to modified Macnab standard when the patients were discharged from the hospital; ② Comparison of the curative effect between the group with intervertebral disc herniation ≥ 0.6 cm and the group with intervertebral disc herniation ＜ 0.6 cm; ③ Comparison of the therapeutic effect of the group complicated with spinal canal stenosis and the group with normal spinal canal; ④ Comparison of the therapeutic effect between the group with good indications and other groups with poor indications.RESULTS: All the 68 patients entered the result analysis. ①The therapeutic effect in the good indication group was obviously better than that in the poor indication group, with a significant difference [85%(29/34),56%(19/34) ,χ2=5.06,P ＜ 0.05]. ② The therapeutic effect was significantly better in the group with intervertevbral disc herniation ＜ 0.6 cm than in the group with intervertevbral discs herniation ≥ 0.6 cm, but without significant difference [75 % (6/8), 50% (4/8),χ2=0.25, P ＞ 0.05]. ③The therapeutic effects were close in the group complicated with spinal canal stenosis and the group with normal spinal canal, but without significant difference [80% ( 12/15 ), 73 ( 11/15 ) ,χ2=0,P ＞ 0.05].④ The therapeutic effect in the group with good indications was obviously better than that in other groups with poor indications, with a significant difference [100%( 11/11 ), 36%(4/11), χ2=5.14,P ＜ 0.05].CONCLUSION: It could improve the therapeutic effects of PLDD when indications were properly chosen.

Objective To evaluate the clinical value of arterial chemotherapy and embolization in the treatment of advanced uterine carcinoma.Methods In this study,52 cases of uterine carcinoma(including 43 carcinoma of cervix and 9 cases carcinoma of endonetrium)were treated interventional therapy.In these cases,after one or two times interventional therapy and after 10-14 days,45 cases followed by uterectomy and pelvic lymphadenectomy. 7 cases that can't operated continue followed by interventional therapy. Results Curative effect was obvious,the clinical symptoms were alleviated,the bleed in operation was decreased and the tumor was divided easily.In 45 cases followed by operation,the one-year and three-year survival rate are 100% and 92%.In 7 cases treated by interventional therapy alone,the one-year and three-year survival rate are 100% and 85%.Conlusion The interventional therapy preoperation can reduce the clinical stage and metastasis of lymph,improve the ectomy by operation and survival rate of patients obviously,so it can be used as a routine treatment in uterine carcinoma.

The fluorescence characteristics of reaction products of sparfloxacin with halide (F, Cl, Br, I) have been studied. It was found that sparfloxacin was oxidized by nitrous acid then reacted with halide further to formstrong fluorescence substances in acid media. Their fluorescence intensitites enhanced with aggrandizing of atom radii of halides, which could emit the strong fluorescence 58～151 fold more than that of sparfloxacin itself. Thus a new sensitive method for the direct determination of sparfloxacin in human urine by derivative-synchronous fluorescence was presented. A plausible mchanism was proposed to explin this behavior.

Objective To evaluate the effect of H. pylori eradication on the histologic changes in the gastric mucosa. Methods H. pylori-positive gastritis patients were divided into eradication group and control group. At the baseline and at the end of follow-up, all patients underwent endoscopy, biopsies for histological test. Result A total of 80 H. pylori-infected patients were divided randomily to eradication group ( n =40) and control group ( n =40). At 6 months, H. pylori eradicated in 29 patients was assigned to eradication group. In the control group, 27 patients remained H. pylori infected. Analysis of paired samples obtained from the same patients showed a significant decrease in acute and chronic gastritis ( P 0.05). In two groups, there were no significant changes in GA ( P =0.53 and 0.64).Conclusion H. pylori eradication can cure the acute and chronic inflammation and reduce, or even reverse the IM.

Objective To compare the operational feasibility and efficacy of inguinal obturator nerve block (ONB) beside the vessel by ultrasound combined with nerve stimulation and traditional ONB guided by nerve stimulation preventing obturator nerve reflex. Methods Forty patients with American Society of Anesthesiology (ASA)Ⅰ or Ⅱ, aged 35~91 years who underwent transurethral electric excision of bladder tumor in our hospital in 2014 were ran-domly divided into beside vessel group (group V, = 20) and traditional group (group T, = 20). Adductor strength was measured before and after the block. Frequencies of obturator nerve block puncture, operation time, visual ana-log scale (VAS) pain score, and incidence of complications were recorded during and after ONB. Results The suc-cess rate of the first puncture was significantly higher in group V than that in group T ( <0.01). The operation time in group V was significantly shorter than that in group T ( < 0.01). The decreased degree of adductor strength has significant difference of the two groups ( < 0.05 or < 0.01). The VAS pain score in group T was higher than that in groups V ( < 0.01). There are two cases contract blood vessel in group T, while none in group V ( < 0.01). There was no case with the local anesthetic toxicity and paresthesia in the area of the obturator nerve and the obtu-rator nerve injury in the two groups. Conclusion Compared with traditional ONB guided by nerve stimulation, in-guinal ONB beside the vessel by ultrasound combined with nerve stimulation showed more accurate positioning, less adjustment, less puncture time, more comfortable and safe.