The complement cascade, as a part of innate immune system, plays a major role in phagocytosis, clearance of apoptotic cells, immune response and inflammation.As an initiator of the classical pathway, C1q not only facilitates apoptotic debris removal but also gets involved in the maintenance of vascular endothelial integrity.As a result, deficiency, excessive consumption or dysfunction of C1q leads to the imbalance of such mechanisms and increases the susceptibility of nephropathy, atherosclerosis and central nervous system diseases.Recenlty, C1q was identified as a new biomarker of aging.C1q could be a useful indicator for early diagnosis, therapy and prognosis.

[Objective] To study the effect of histone deacetylase inhibitor-Trichostatin A(TSA) on proliferation and differentiation of human osteogenic sarcoma cell. [Methods]Human osteogenic sarcoma cell line MG-63 was treated in vitro with various concentrations of TSA, a potent and specific histone deacetylase inhibitor. Proliferation suppression was observed by MTT method and inverted microcopy before and after TSA treatment, and the cell growth curve was obtained. The cell growth and invasion ability were measured with the colony-formation rate in soft agar test. Flow cytometry was used to investigate the cell cycle.[Results]TSA significantly inhibited the proliferation of the human osteogenic sarcoma cell in a dose-dependent and time-dependent manner. The cell experienced benigh morphological differentiation. The colony-formation rate in semi-solid agar was significantly decreased(P

Fox family transcription factors have crucial biology functions,including the regulation of proliferation,differentiation and tumorigenesis.Deregulation of Fox proteins expression may act as both oncogenes and tumor suppressors,the relevant researches have been paid more and more attentions.Here,this review focuses on the roles of Fox family genes in oncogenesis from the articles published recently.

Objective To investigate the possibility of reserving uterus during severe postpartum hemorrhage. MethodsA retrospective analysis was conducted on the clinical data of 138 cases of severe postpartum hemorrhage (blood loss ≥2000 ml) from January 1, 2003 to December 31, 2009. Results Among 138 cases of severe postpartum hemorrhage, uterine atony (n= 60, 43. 38 ％ ) was the first cause and the second was placental factor (n= 55, 39.86 ％). The blood loss varied from 2000 ml to 10 000 ml and the mean level was about (3004± 1473) ml. The volume of blood transfusion for these patients varied from 800 ml to 7200 ml. Among these patients, the blood loss of 108 cases reserved uteri was from 2000 ml to 7500 ml, with the average of (2564±932) ml； while for 30 cases performed with hysterectomy, the blood loss was about 2500 to 10 000 ml averagly (4653± 1857) ml (t=8. 57, P=0.00). These patients were divided into two groups according to time series. Twelve cases of hysterectomy were performed during 2003to 2005, and the hysterectomy rate was 0. 47‰; 18 cases of hysterectomy were performed during 2006 to 2009, and the hysterectomy rate was 0. 36‰. The average blood loss of the above two groups was (3783±861) ml and (5233±2124) ml respectively (t=2. 234, P=0. 034). Among all the cases with blood loss ≥ 3000 ml, uteri were reserved in 24 cases with the average blood loss of (3818 ± 1284) ml； while hysterectomy were performed in 27 cases with average blood loss of (4900 ± 1789) rnl (t = 2. 453, P =0. 018). The time for blood loss to 3000 ml in the two groups was (160±129) min and (100±67) min,respectively, and the difference was significant (t=2. 113, P = 0.04).The uteri of six cases with postpartum hemorrhage over 4000 ml were successfully reserved, and average bleeding amount was 5570 ml.Two patients among the 138 women died of amniotic fluid embolism.The perinatal mortality rate was 3. 73％.ConclusionsThe postpartum hemorrhage volume and velocity is the key point to decide whether to reserve the uterus or not.For the population with high risk factors,prophylaxis treatment with prostaglandins should be initiated to reduce the bleeding volume.Uterine packing might be an effective treatment to stop postpartum hemorrhage, especially for those bleeding due to placenta previa.

Hepatic resection is considered curative treatment for patients with hepatocellular carcinoma (HCC),but the range of indication is narrow. Liver transplantation(LT) is another option, but recurrence and metastasis reduce long-term and medium-term survival. Appropriate patient selection is crucial in ensuring acceptable outcomes. Novel anticancer agents (chemotherapy and molecular target therapy) and appropriate use of immunosuppressant hold promise for improving the outcomes.

Objective To establish a method of HPLC assay for determining emodin in Yishi Granules.Methods The effective components in Yishi Granules were determined by HPLC.The chromatographic conditions were as follows:Alltech C18 column(250 mm? 4.60 mm,5 ? m),mobile phase consisting of methanol-0.1 % phosphoric acid(80:20),flow rate at 1 mL? min-1,detecting wavelength being 254 nm,and the column temperature being 30 ℃.Results The separation and the linearity were good in the range of 32.8～ 131.2 ?g,r=0.999 9.The average recovery of emodin was 100.20 %,and RSD=1.52 %(n=6).Conclusion The quantitative method for determining the active components of Yishi Granules is simple,feasible and reproducible,and is beneficial for the quality control of Yishi Granules.

Nonalcoholic fatty liver disease(NAFLD) is the most common liver disorder in the world.A significant,albeit relative small,proportion of NAFLD patients can advance from steatohepatitis to liver cirrhosis and hepatocellular carcinoma.Moreover,NAFLD is associated independently with an increased incidence of type 2 diabetes and cardiovascular disease.The mortality of patients with NAFLD is significantly higher than that among the general population and cardiovascular risk may compete with liver-related risk in dictating the final outcome.From a clinical point of view,it has become mandatory to evaluate the metabolic risk factors in NAFLD patients and to consider careful surveillance and aggressive treatment of both hepatic and cardiovascular outcomes.Nowadays,there are substantial advances in the ability to make the diagnosis of NAFLD as well as both grade and stage the disease,however,liver biopsy remains the gold standard.Lifestyle changes are the first line and mainstay of management of NAFLD,weight loss and treatment of insulin resistant remain central to the therapeutic process.Specific pharmacological treatment is currently not recommended for routine clinical practice.

BACKGROUND:Nonunion is a common clinical problem in the prognosis of tibial fracture. The treatment method of tibial fracture nonunion is extensive and develops rapidly. Different repair plans should be taken to the nonunion of tibial fractures caused by different factors. OBJECTIVE:To summarize the repair strategy and effect of adjuvant therapy of internal fixation implants and external fixation stent for treatment of tibial fracture nonunion. METHODS:The PubMed database and CNKI database were retrieved by the author of this article for the published articles related to reason and treatment method of nonunion. The key words were “tibial fracture nonunion, reason,treatment, implant, external fixation”. The repetitive and old articles were eliminated. RESULTS AND CONCLUSION:(1) Finaly 35 articles were selected for further analysis. (2) Surgical method is the chief treatment for tibial fracture nonunion. We choosedifferent surgical methods for the best curative effect according to the reasons of nonunion and whether it is infected or not. The biological and structural factors that cause bone delayed healing should be considered in the treatment. (3) The fixed treatment on the structure is divided into external fixation and internal fixation. Intramedulary nail and compression plate can give stability of nonunion without infected tibial fractures, and it is a good solution to the structural problems. External fixation can be used for nonunion of infected tibial fracture. (4) To solve the biological problems of nonunion of tibial fractures (bone defect), various bone grafting materials and bone grafting techniques can be used. It is generaly required to fix the bonegraft in the treatment of nonunion of the tibia fracture. In recent years, biological materials have been gradualy applied to solve the problem of bone nonunion of tibial fractures. Although electrical stimulation and ultrasound have been shown to promote fracture healing, it has not been clinicaly proven to be used in the treatment of nonunion of tibial fractures.

[Objective]To explore the effect of external fixator combined with vaccum sealing drainage(VSD) for the treatment of infection after internal fixation in tibial and fibular fracture.[Method]Thirty-six patients with infection after internal fixation in tibial and fibular fracture were treated by external fixator combined with VSD from June 2004 to June 2008.[Result]During the following-up period from 8-30 months in thirty-two patients,the infection in all cases were controlled effectively,no case of recurrence was found in all patients,and satisfactory healing were found in 32 cases.The average time of fracture union were 4.5 months;the removal time of external fixation were 5 months on average.[Conclusion]The combined operative procedure is simple,reliable and less traumatic and allow to early functional exercises.Therefore,it is a satisfactory measure for the infection after internal fixation in tibial and fibular fracture.

Due to unknown onset time, patients with wake-up stroke and unknown onset stroke areoften excluded from thrombolytic therapy. However, many clinical studies have shown that multimodal imaging techniques can effectively guide wake-up stroke and unknown onset stroke for intravenous thrombolysis. Its endovascular treatment has also made some progress. This article mainly reviews the imaging findings, intravenous thrombolysis, and endovascular therapy in patients with wake-up stroke and unknown onset stroke.