Visual cognition is a comprehensive procedure of information processing from eyes,visual pathway through cerebral cortex.Visual function covers down multiple concepts of ocular structures,psycophysics,photochemistry and photoelectric information transformation,reflecting visual acuity,contrast visual acuity,visual field,color vision,binocular vision,visual perception and many other detailed parameters.Ocular abnormal status is reflected to certain visual function while visual function is various and anfractuous from kinds of ocular problems.These complex networks lead to some misunderstanding of research in the filed of visual function concept.An expatiation of the diversified visual function is helpful for us to integrate visual function and ocular ailment process.This paper tried to explicit some misconceptions,and accentuated the importance of clinical research around visual function for the diagnosis and treatment of eye diseases and nervous system diseases.

Objective We tried to investigate the current status of pediatric nursing post setting and analyze the practical application effect in the clinical working practice for the foundation of readjusting and setting up the pediatric nursing positions.Methods By convenient sampling method,the general condition and current post setting situation in the pediatric wards of 7 hospitals with self-designed questionnaires were investigated.The results underwent analysis Results The nursing post setting in pediatric wards of 7 hospitals were not all the same,nursing posts in the pediatric ward included three categories:management positions,teaching positions and clinical positions.Management positions included the head nurse,deputy head nurse and head nurse assistant; teaching positions included the teaching secretary and clinical total teaching nurse; the seven kinds of clinical nursing positions were office job,day shift,night shift,evening shift,midnight shift,late-morning shift,auxiliary job.Though some nursing jobs differed in the position names and working hours,their job contents were nearly identical.Conclusions Pediatric nursing post setting management in our country is still in the exploratory stage at present,it has not form a unified perfect job management system.Pediatric nursing positions should be set from the perspective of evidencebased theory,satisfying the demands of patients as the guidance,coming from clinical needs as the first principle,in the full implementation of the accountability for nursing and based on comprehensive consideration of various factors,so that the post setting can be scientific and reasonable.

Objective To investigate the correlation between the HSP90 expression and invasion and metastasis potential of human breast cancer cell line. Methods The mRNA expression level of HSP90 was detected by RT-PCR. The protein expression level of HSP90 was measured by Western blotting. The effects of HSP90 inhibitor Geldanamycin (GA) on the invasion and motility abilities of human breast cancer line MDA-MB-231 and MDA-MB-435s in vitro were explored by transwell chamber. Results The mRNA expression level of HSP90? in MDA-MB-435s cell was higher than that of MDA-MB-231 cell (P0.05) between the two cell lines. The protein expression level of HSP90 in MDA-MB-435s cell was higher than that of MDA-MB-231 cell (P0.05). After treated with GA, the invasive and metastatic potential in human breast cancer cell MDA-MB-435s and MDA-MB-231 was suppressed significantly (P

To construct the eukaryotic vector that expresses the fusion protein of Axud1 and influenza virus hemagglutin HA epitope tag, the total RNA was isolated from the peripheral blood lymphocytes, and reverse transcription reaction was used to amplify the full length of human Axud1 cDNA. PCR product of Axud1 was then amplified using specific primers containing HA epitope sequence, and inserted into eukaryotic expression plasmid pcDNA3.1(+)digested with BamH Ⅰand Xba Ⅰ. The recombinant plasmid was identified by polymerase chain reaction, restriction endonuclease mapping and sequencing, and then transfected into human lung adenocarcinoma SPC-A1 cell lines.The fusion HA-Axud1 protein expression in anti-G418 clones was verified by Western blot. This study might be instrumental in further study of the function of Axud1 protein in tumor cells.

Computer laboratory teaching is the most important method to train the computer operating skills of medical students.Therefore,we have attempted the student-centered open-laboratory teaching model.This teaching model can train the computer operating skills and creative ability of students in medical college.

Objective To investigate the effect of dexmedetomidine (Dex) on the apoptosis and respiratory burst of human polymorphonuclear neutrophils (PMN) in vitro. Methods The peripheral venous blood was collected from healthy volunteers. Isolation of PMN was performed by using the discontinuous plasma-Percoll gradient technique. PMN was randomLy divided into four groups: control group and three different concentrations of Dex (1 ng/mL,10 ng/mL,100 ng/mL) groups. PMN was cultured in enriched RPMI-1640 media at 2 × 106/mL for 24 h. The caspase-3 activity, apoptosis rate and respiratory burst of PMN were detected at 2 and 24 h. Results Compared with the control group, the PMN which was treated with three different concentrations of Dex showed no significant difference in caspase-3 activity, apoptosis rate and respiratory burst of PMN after 2 h of incubation. Compared with the control group, 1 ng/mL concentration of Dex did not affect the caspase-3 activity, apoptosis and respiratory burst of PMN cultured for 24 h. However, 10 ng/mL and 100 ng/mL concentrations of Dex promoted the caspase-3 activity, increased apoptosis rate and reduced the respiratory burst of PMN after 24 h of incubation. Compared with 10 ng/mL Dex group, 100 ng/mL concentrations of Dex promoted the caspase-3 activity, increased apoptosis rate and reduced the respiratory burst of PMN after 24 h of incutation. Conclusion Clinically relevant concentration of Dex does not affect apoptosis and respiratory burst of PMN, while high concentration of dexmedetomidine can induce apoptosis of PMN.

Objective To analyze the efficacy and safety of combination of rh-endostatin (Endostar) with docetaxel treatment on patients of non-small cell lung cancer (NSCLC) who presented progressive disease (PD) after first-1ine chemotherapy.Methods From Janury 2010 to March 2013,76 patients with stage Ⅲ B/ Ⅳof NSCLC experienced previous chemotherapy of one-regimen were screened for this trial.Given docetaxel combined with recombinant human endostatin (combined group, 38 cases) and docetaxel (chemotherapy group, 38 cases) to continue treatment.The response, time to progression(TTP) and adverse effects were observed in both groups.Results The objective response rate (ORR) and clinical benefit rate (CBR) were 0 and 63.2％ (24/38) in the combined group, along with 0 and 52.6％ (20/38) in the single docetaxel group, with a nonsignificant difference between the two groups (P =0.712).The median TTPs in the combined and single docetaxel groups were (2.6±0.4) months and (2.0±0.8) months respectively (P =0.083).The median TTPs of the patients with SD after therapeutic cycles in the combined and single docetaxel groups were (6.2±0.4) months and (3.2 ± 0.8) months respectively (P =0.038).The differences between two groups were nonsignificant in adverse, serious adverse and cardiovascular adverse effects(P＞0.05).Conclusion Endostar may prolong TTP in patients with advanced NSCLC benefited from docetaxel treatment without increased toxicities.

This paper introduced the concept of wearable medical equipment, the development trend, a parti-cular way of gathering information and privacy protection from residential to people, to the development trend of da-ta-centric. Thus, this paper analyzes the information privacy protection of ethical challenges are: data has been weakened by the autonomy of the parties, the right to self-determination; The double-edged effect of wearable medical equipment increases the difficulty of the privacy protection, Has not been perfect wearable medical equip-ment behavior increase the difficulty of privacy protection. Privacy protection facing the challenge of wearable medi-cal devices for causes mainly include:commercial interests drive;Privacy violation cost greatly reduced. Doctor-patient information asymmetry, the patients privacy protection consciousness is weak. To solve above problems, and puts forward the countermeasures of information privacy protection:perfect wearable medical equipment's ethics and policies and regulations;improve the wearable medical devices the user's ego to protect consciousness;improve the wearable technology protection capabilities of medical equipment.

Mast cells (MCs) are bone marrow-derived and tissue-homing immune cells which have important functions.Recruitment and activation in tumors are demonstrated to be mainly mediated by tumorderived stem cell factor (SCF) and its receptor c-kit on MCs.The numbers and distributions of MCs in tumors are closely related to the growth of tumors and angiogenesis.MCs can mediate angiogenesis by releasing a series of angiogenesis factors,so as to promote the progress of tumors.