OBJECTIVE:To put forward some suggestions for the development of e-commerce in China's pharmaceutical retail chains.METHODS:To drawn on experiences from the western developed countries in developing B to C pharmaceutical e-commerce and analyzed the problems existing in China's pharmaceutical industry.RESULTS & CONCLUSIONS:To develop e-commerce in China's pharmaceutical retail chains,the cooperated supervision of the concerned governmental departments is indispensable,in addition,the level of informatization should be improved step by step and the network of retail chain should be relied on to offer customer-centered services.

Cloning, Molecular ; methods ; Dental Caries ; immunology ; prevention & control ; Forecasting ; Humans ; Research ; trends ; Research Design ; Vaccination ; Vaccines ; genetics ; immunology

OBJECTIVE: To propose some suggestions towards the operation and development of online pharmacies in China.METHODS: From the following three aspects: website design,information provision,and customer service,the paper introduced and compared the websites of three approved online pharmacies,which include Jingwei Pharmacy,Shanghai Pharmacy and Jinxiang Pharmacy.RESULTS & CONCLUSIONS: To develop online pharmacies,the information display should be customer-centered,new marketing plans based on the characteristics of the internet should be developed;meanwhile,the operators of online pharmacies should help consumers developing an awareness of drug safety and offer them corresponding services.

Dental Pulp Cavity ; anatomy & histology ; Humans ; Root Canal Preparation ; methods

Dental Pulp Cavity ; abnormalities ; pathology ; Humans ; Root Canal Therapy ; methods

Humans ; Periapical Periodontitis ; diagnosis ; etiology ; therapy ; Root Canal Therapy ; adverse effects

Humans ; Root Canal Obturation

Humans ; Root Canal Therapy ; instrumentation ; methods

China ; Humans ; Root Canal Therapy

Objective To investigate the effect and underlying mechanism of Caudatin combined with Gefitinib on Gefitinib resistance induced by HGF in PC-9.Methods Model of EGFR-TKIs resistance in PC-9 cells was induced by exogenous HGF and co-cultured with MRC-5.Caudatin was tested as a drug resistant modulator to reverse the resistance of Gefitinib in PC-9 cells induced by HGF by MTT assay.Western blot was performed to observe the mechanism of Caudatin combined with Gefitinib reversing the resistance of PC-9 induced by HGF.Results The resistance of gefitinib to PC-9 was induced by exogenous HGF and co-cultured with MRC-5 which could reduce relative inhibitory rate ( P<0.05 ) .Neither caudatin ( 0-32 μM ) or Gefitinib (1μM) alone could significantly inhibit proliferation of PC-9 in the presence of HGF, which could be inhibited in a dose-dependent manner by Caudatin combined with Gefitinib ( P<0.05 ); Caudatin combined with Gefitinib down-regulated the phosphorylation levels of Met and PI3K/Akt simultaneously (P<0.05).Conclusion Caudatin could reverse the drug resistance of Gefitinib in PC-9 induced by HGF, the mechanism of which may be related to the inhibition of Met/PI3K/AKT pathway.