OBJECTIVE:To study the combined antibacterial activity of Rhizoma Coptidis,Bee Propolispro and Fructuts Evodiae against Helicobacter pylori(Hp).METHODS:The protocol was designed by checkboard method.The MICs of Rhizoma Coptidis,Bee Propolispro and Fructuts Evodiae alone or in two-and-two combination against Hp were determined by agar plate method,and the FIC indexes was calculated for the evaluation of the combined effects.RESULTS:When Rhizoma Coptidis combined with Bee Propolispro,the FIC index was 0.16(0.5)when combined with Fructuts Evodiae;The FIC index was 0.05(

OBJECTIVE:To select the best medium prescription of HP.METHOD:To select the best solid medium of HP by orthogonal test design and to study the condition of preserving HP.RESULTS & CONCLUSIONS:There are significant deviations of growing circumstance of HP on the different cultivation medium.The best solid cultivation medium for HP is colombian agar or brain heart infusion agar medium with 2.5%mixed antibiotic added with 5.0%bovine serum and kept the pH of medium at 7.5.HP is preserved under —70 ℃～—80 ℃,which could be successfully antibiotic.

OBJECTIVE: To construct hospital pharmaceutical website and facilitate the evolvement of hospital pharmaceutical care.METHODS: Website management system was developed independently and the system was used to construct hospital pharmaceutical website in our hospital.RESULTS & CONCLUSIONS: Website management system which was designed with B/S three-tier structure which sustains multi-level management authority control and a variety of database and runs stably.The professional website based on website management system is convininent and maintenance,rapid in operation,cheap in cost,low in consumption of human resouces,comprehensive in function,highly professional,and it has greated enhanced the working efficiency in hospital pharmacy.

Objective To investigate the clinical effects of prostaglandin E1 (PGE1) in the treatment ot chronic renal failure in the 2nd ～ 4th period.Methods 128 patients with CKDs at stage 2 to 4 were selected and randomly divided into the observation group and control group,64 cases in each group.Patients in control group were given routine drug treatment,and patients in observation group were given PGE1 on the basis of the control group.They all treated for 3 months.Renal function indicators before and after treatment,curative effect and adverse reaction were compared.Results There were no statistical differences in renal function indicators before treatment in two groups (t =0.27 ～ 0.95,P ＞ 0.05).After treatment,the 24 hours urinary protein (t =3.41,P ＜ 0.05),creatinine clearance rate (t =4.04,P ＜ 0.05),serum creatinine (t =4.15,P ＜ 0.05),serum urea nitrogen (t =3.92,P ＜ 0.05) between the two groups had statistical differences.Total effective rate of the observation group was 84.37％,which was significantly higher than 57.81％ of the control group (x2 =5.63,P ＜ 0.05).There was no adverse reaction observed in observation group.Conclusion PGE1 might improve patients' proteinuria and renal function in the current situation of lacking effective treatment.

With the development of conformal radiotherapy,radiotherapy has been used not only in localized prostate cancer as curative therapy.In the treatment of locally advanced prostate cancer,adjuvant therapy after radical prostatectomy and combination therapy with androgen can be used,and can relieve the patients with advanced prostate cancer.The indication,efficiency and side effects of the different techniques are reviewed as follows.

Small intestinal submucosa has been applied in a variety of areas of basic and clinical study with its Characteristics of anti-infective and Low immunogenicity and so on.However,it is common to find the shrinkage and other problems in some tissue repair,and affects the functional recovery of repaired tissue to a certain extent.Thus,it is still a question to be resolved that how to avoid the shrinkage and other adverse effects after tissue repair.This paper provides a reliable basis for further correct understanding of the SIS's capacity of tissue repair by reviewing and summarying its characteristics and present situation of tissue repair,especially put forwarding the shrinkage problem.

Dendritic cells (DCs)can capture HIV from the mucosal site and carry it to the draining lymph nodes to promote CD4 + T cell infection.DC-SIGN(CD209)plays a vital role during this process. Heroin,methamphetamine and cocaine are the most abused drugs used by drug abusers,which can increase the opportunities of HIV infection.Drugs acting on the corresponding receptors activate the downstream signaling pathway and regulate the expression of DC-SIGN on the surface of DCs,weakening the inhibited effects of immune cells on HIV and promoting HIV infection in many aspects.Given the multiplex effects of drugs on immune function,this article reviews the relationship between abused drugs,DCs and HIV infection,particularly in the signal pathway on immune modulation.

OBJECTIVE:To probe into the characteristics and general pattern of new ADR in our hospital. METHODS:A to-tal of 151 new ADR cases reported to Nationwide ADR Monitoring Network by our hospital during 2013 to 2014 were analyzed statistically. RESULTS:Of the 151 ADR cases,males(56.29%)showed higher proportion than females(43.71%);most of ADR cases were induced by anti-infective agents,including 45 cases(29.80%). ADR cases most easily induced by injection and intrave-nous dripping,accounting for 83.44% and 91.39 %,respectively. Lesions of skin and its appendants were most common clinical manifestations of ADR,which accounted for 28.82%(66 cases). There were 10 new and severe ADR cases(6.62%). All of ADR cases were cured or improved. CONCLUSIONS:More attention should be paid to ADR monitoring especially indication of anti-in-fective agent to reduce the occurrence of ADR.

[Objective]This paper will pitch-in TCM psychosomatic theory(from Huangdi Neijing),exploring the disorders of depression with the metabolic syndrome and pathogenesis of traditional Chinese medicine,so as to provide effective ideas for the treatment of depression with metabolic syndrome.[Methods]:Through expansion on traditional chinese medicine's basic understanding of depression with metabolic syndrome,utilizing basic TCM viscera-state theory, from the causes of this disease,key factors,pathogenesis transformation three aspects to discuss the pathogenesis of the disease. [Results]By discussing the heart,liver,spleen function of zang-fu organs and their mutual relations in the role and impact of the disease development process, this paper thinks of the nature of disease as asthenia in origin and asthenia in superficiality,which locates in spleen,has association with heart and liver, phlegm, qi and blood stasis is the disease of pathological product of pathological factors to promote the development of the disease.Kidney would be involved in it with long course of disease.The therapy could focus on tonifying spleen,nourishing heart and soothing liver,with methods of promoting the circulation of qi,removing blood stasis and reducing phlegm,meanwhile looking out to protect kidney.[Conclusion]Disorder of depression and pathogenesis of TCM of metabolic syndrome,to guide the clinical treatment of the disease and the theory of traditional Chinese medicine development has practical significance.

The development of clinical microbiology laboratory is an essential part in promoting the development of clinical microbiology and the basis rational use of antimicrobial agents.Under the circumstances of national specific rectification of antimicrobials,clinical microbiology laboratory should focus on self-buildup and improvement.The development of clinical microbiology laboratory should focus on development of both software and hardware capabilities for lab examination and diagnosis in an all-round way,enhance the antimicrobial susceptibility surveillance,and emphasize and communication and cooperation between laboratory and other departments e.g.clinical departments,pharmacy department and nosocomial surveillance and control office,making concept translation from past ‘paramedical department' to a ‘platform department',contribute its due share to the rational uses of antimicrobial agents.