Femoral Fractures ; surgery ; Fracture Fixation ; Humans ; Minimally Invasive Surgical Procedures

The invasion and metastasis of esophageal carcinoma is associated with several tumor marker on oncogenesi and tumor development,including matrix metalloproteinase,cell adhesion molecules CD44,pituitary-tumour transforming genes.Studies have shown that these tumor markera are over-expression in esophageal carcinoma,which affect the progress and prognosis of esophageal carcinoma,and their specificity and value for clinical application need to be futher studied.

Objective To evaluate the clinical effect of ultrashort wave combined with dephlogisticate cholagogue table in treating patients with chronic cholecystitis.Methods One hundred and forty-four patients with chronic cholecystitis treated from January 2009 to October 2011 were randomly divided into 2gtoupe by mechanical sampling method.Treatment group (76 cases) was treated with ultrashort wave and dephlogisticate cholagogue table.Control group (68 cases) was treated with dephlogisticate cholagogue table.The therapeutic effect and the incidence of ache and fever and B-type ultrasonography between two groups were compared.Results The total effective rates were 89.5％ (68/76) in treatment group and 67.6％(46/68) in control group.There was significant difference (P ＜0.01).The incidence of ache and fever,positive rate of B-type ultrasonography in tneatment group [10.5％(8/76),5.3％(4/76),10.5％(8/76)] were significantly lower than those in control group [32.4％ (22/68),14.7％ (10/68),33.8％(23/68)] (P ＜ 0.05).Conclusion Ultrashort wave combined with dephlogisticate cholagogue tabble is an effective method for treating chronic cholecystitis.

Purpose　To evaluate the therapeutic effect of laser-induced chorioretinal venous anastomosis for retinal vein occlusion　(RVO)．　Methods　The investigation included 28 consecutive patients　(28 eyes) with RVO．The non-ischemic group 18　cases　(18 eyes) were treated with the krypton red laser to induce chorioretinal venous anastomosis， the ischemic group underwent the same therapy and the grid or scatter-type photocoagulation at the same time． All of the eyes were followed up for more than 5 months， and with an average period of 6．6 months．　Results　Seven cases　(7 eyes， 38．9%) of the non-ischemic group created successful chorioretinal venous anastomosis， with mean improvement of 4．43±0．78　standard deviation　（±s） lines of best-corrected visual acuity compared to　0．19±0．67　（±s） lines for 11 eyes with unsuccessful anastomosis　(P<0．001)． None of the seven eyes developed to ischemic state． Four of the 11 eyes with unsuccessful anastomosis converted to ischemic type (36．4%)． Two cases of the ischemic group created successful anastomosis with improvement in visual acuity． The other cases had no change in visual acuity．　Conclusion　Laser-induced chorioretinal venous anastomosis is a new therapeutic method for RVO， especially for nonischemic type　and successful anastomosis can decrease the conversion rate of the vein occlusion to an ischemic state．

Objective To explore the mechanism of vitamin E on delaying skin aging by observ-ing the expression of hyaluronic acid synthetase-2 (HAS-2) in human dermal fibroblasts in vitro. Methods Human skin fibroblasts were cultured in vitro, and these fibroblast cells were then divided into 3 groups: different concentration of vitamin E (0, 0.1 × 10-10, 1 ×109mol/L) was added in the medium in the different group. 24 hours later, the fibroblasts were collected, RNAs extracted, and then amplified by RT-PCR. The PCR product was determined by agarose gel electrophoresis, to analyze the level of HAS-2 mRNA expression. Results RT-PCR showed the lever of HAS-2 mRNA was higher in the low-dose group than the control group, with significant difference (P<0.05) ; the lever of HAS-2 mRNA was higher in the high-dose group than control group, with significant difference (P<0.05). There was no significant difference in the lever of HAS-2 mRNA between the low-dose group and the high-dose group. Conclusions Vitamin E can enhance the expression of hyaluronic acid synthetase-2 mRNA, may increase the synthesis of HAS in skin fibroblasts and increase water content in the skin, so that it might reverse or delay the skin aging.

MicroRNAs can post-transcriptionally regulate the expression of their target mRNAs. MicroRNA-126 plays an important role in tumorigeness by targeting EGFL7, HOXA9, IRS-1 and p85-β. Intriguingly, it is downregulated in a wide range of tumors and is testified that it functions as a tumor suppressor in lung cancer, leukemia, breast cancer and ovarian cancer.

Attenuated Salmonella can be used not only as live vaccines but also as vectors for exogenous genes. As vectors, attenuated bacteria can deliver genes directly to tumor tissues, leading to the expression of the cloned genes and induction of specific anti-tumor immunologic responses to inhibit tumor growth. Using attenuated Salmonella as a vector offers a new approach for vaccine development.

By analyzing the current situation of the application of clinical pathways in China, the paper reviews the achievements that have been made via the application of clinical pathways to the domestic management of patient care, nursing, pharmacy and operation and discusses the problems existing in such application. It argues that there are sound prospects for the application of clinical pathways to hospital management.

CTGF, a member of the CCN family of immediate early genes, is a recently discovered profibrotic growth factor, which is involved in many pathophysiologic procedures. CTGF acts as a downstream effector of TGF-? acting on interstitial cells to enhance the progression of fibrotic renal diseases. It has been shown that CTGF gene expression can be induced or blocked by some kinds of cytokine and drugs. It is an interesting candidate target for future intervention strategies of renal interstitial fibrosis. [

A method for simultaneous isolation and clean-up pyrethroid insecticides (methothrin, fenpropathrin,cyhalothrin, permethrin, cypermethrin, fenvalerate and deltamethrin)with a solid phase extractionmethod (Sep-Pak C18 cartridge) from biological samples (human plasma and urine) is presented.The systematic separation of the insecticides was excellently performed by wide-bore capillary gaschromatography with flame ionization detection and temperature programming. The recoveries werebetween 81% and 93% for plasma, and 90% and 102% for urine. Mixing the samples with 70%methanol and eluting with chloroform demonstrated excellent adsorption on C18 and the best recoveries,respectively.