True neoplasm of retinal pigmented epithelium (RPE) is rare, but because of important differential diagnosis including malignant melanoma (which is more common than adenocarcinoma of RPE), both in clinical and pathological characteristics, we are interested in reporting this case. A 60-year-old man underwent right eye enucleation because of progressive loss of vision and ophthalmologic diagnosis of malignant melanoma. Pathological findings revealed a lesion in posterior segment near the optic nerve. The mass was solid and well circumscribed. In histological evaluation, the tumor was composed of cells having large, pleomorphic and hyperchromatic nuclei, prominent nucleoli and occasional pigmentation. Tumor cells were mostly arranged in papillary pattern. For differentiation of melanoma, immunohistochemistry was done. Epithelial membrane antigen (EMA)was strongly positive and HMB45 was negative; consistent with the diagnosis of adenocarcinoma of RPE. Systemic evaluation ruled out any metastasis. In conclusion, although adenocarcinoma of RPE is rare, but must be kept in mind in differential diagnosis of malignant melanoma of choroids.

Background: In this study, we demonstrated the effects of the Gallic Acid (GA) molecule on the prostate cancer cells line PC3 using the comet assay (Alkaline electrophoresis) technique and its effects on some important apoptotic factors including BAD (Bcl-2-Associated Death promoter), BAK (Bcl-2 homologous Antagonist/Killer), and BIM (Bcl-2-like protein 11) via simulation analysis by using the Auto Dock and Gromacs software. Methods: Following the MTT assay on the PC3 cells, and determining IC50, we used three concentrations of GA to around IC50 to treat PC3 cells. 100 comet pictures were obtained by alkaline electrophoresis and have been analysed with the CASP version 1.2.2 software; all the results were thereafter analysed by the SPSS version 21 statistical software. Results: The IC50 value for GA was determined to be 35 μM. The ratio of tail to head in alkaline electrophoresis for the three concentrations below the IC50 of GA in 25, 30, and 35 μM were measured as 24.7 (2.7), 44.5 (1.8), and 57.3 (1.3) percent, respectively. The results of the preapoptotic factors (BAD, BAK, and BIM) in the performed simulation in the absence and presence of GA showed that the GA protein causes the structural instability in the BAD protein, and the effect of GA can be explained by the creation of hydrogen bonds with proteins. Conclusion: GA is a polyphenol compound in plants that can suppress cell growth and induce apoptosis in PC3 cells in prostate cancer in the range of IC50 concentrations. The apoptotic properties of GA induce pre-apoptotic factors.