ObjectiveTo study the heat shock protein 47 ( HSP 47 ) andtype Ⅰ ,Ⅲ collagen relevance expression in keloid tissue. MethodsUSE immunohistochemistry to detect 21 patients( HS group) pathological keloid tissue HSP47, Ⅰ-type, Ⅲ collagen levels,and normal foreskin(control group) compared to the expression of HSP 47 and Ⅰ and Ⅲ collagen. ResultsHS group HSP 47 scar absorbance was( A value) ( 133.0 ± 58.7) ,it was significantly lower than the control group( 169.6 ± 53.2) ( t =2. 697 ,P =0.024) ;two groups of collagen type Ⅰ, Ⅲ collagen levels had statistically differentce(t =14.898,12.610,all P =0.000) ; HSP 47 level and type Ⅰ collagen were positively correlated(r =3.058,P =0. 034),and type Ⅲ collagen content was also positively correlated(r =4. 610, P =0. 029). ConclusionHSP 47 in keloid tissue could cause dermal layer assembly of collagen synthesis and significantly increased,and it was an important mechanism for the formation of keloids.

PRL-3 is a key gene related to metastasis of colorectal carcinoma. However, it is known little about the possible regulatory mechanisms of PRL-3 gene expression. There were three possible promoter regions predicted by TRED, a promoter prediction software,which were all located in the upstream regions of PRL-3 gene. One of PRL-3 gene candidate promoters was located in the region of about -1kb upstream proximal to 5′ UTR of PRL-3 gene. Many possible transcription factor binding sites such as Snail, n-MYC, ARNT, E74A, NF-kappaB, NRF-2 and AML-1 were predicted in the region by Consite, a promoter analysis web system. Interestingly, a 5′ CACCTG 3′ core sequence and other related sequences of snail binding sites were found in promoter region of PRL-3 genes. Two PRL-3 gene promoters between -699 to 299 nt and between -642 to -383 nt were cloned into pGL3 vector with luciferase report gene. Both of them had promoter activities in four different cell lines including colorectal carcinoma cell lines SW480 and SW620, nasopharyngeal carcinoma cell line CNE2 and human embryo kidney cell line 293A. Interestingly, the luciferase activities of the short DNA fragmentations with Snail binding site′s core sequence 5′ CACCTG 3′ were higher than that of the longer one. PRL-3 promoter obtaining the 5′ CACCTG 3′ core sequence of Snail binding sites, was validated to bind to snail by chromatin immunoprecipitation (CHIP) analysis and electrophoretic mobility shift assay (EMSA) in SW480 cells. The data suggested that Snail was involved in regulation of PRL-3.

Objective:To analyze the gene mutation types and clinical hematological phenotypes of abnormal hemoglobinopathy in couples of childbearing age in Nanhai District, Foshan.Methods:The hemoglobinopathy gene sequencing, blood routine testing, and hemoglobin capillary electrophoresis results of 24 748 samples from prenatal and premarital examinations at Nanhai Maternity and Child Healthcare Hospital of Foshan from August 2017 to November 2023 were retrospectively analyzed.Results:Totally 41 abnormal hemoglobinopathy genotypes were detected in 294 cases, the detection rate was 1.19% (294/24 748). There were 24 genotypes involved in α globin structural variation, with 172 cases. There were 13 genetypes involved in β globin structural variation, with 118 cases. There were 4 genotypes involved in α + β globin structural variation, with 4 cases. Among them, Hb New York (82 cases), Hb Hekinan Ⅱ (76 cases), Hb Owari (29 cases), and Hb Q-Thailand (27 cases) were the most common genetypes. Among 274 cases of abnormal hemoglobinopathy gene carriers, 89 cases were positive in blood routine phenotype, the detection rate was 32.48%, including 81 cases with abnormal mean corpuscular volume (MCV) and 71 cases with abnormal mean corpuscular hemoglobin (MCH). There were 166 cases with positive phenotype in hemoglobin capillary electrophoresis, the detection rate was 60.58%, among them 81 cases with abnormal HbA 2 and 133 cases with abnormal hemoglobin band. Most of the abnormal hemoglobinopathy gene carriers without combined α or β-thalassemia had no clinical symptoms or only mild clinical symptoms, and a small number had positive hematological phenotypes. Conclusions:The incidence of abnormal hemoglobinopathy is relatively high among couples of childbearing age in Nanhai District, Foshan, and the main variation is α globin structure variation. Some genetypes are positive for hemoglobin capillary electrophoresis, and a few gene types show anemia symptoms.