1.Effect of Retention-Enema of Bowels-Unblocking and Heat-Purging Mixture Combined with Electro-acupuncture for Peritonitis After Operation: An Observation of 32 Cases
Jianxing XIE ; Fabin XU ; Yong FANG
Journal of Guangzhou University of Traditional Chinese Medicine 2001;0(03):-
To investigate the effect of retention-enema of Bowels-Unblocking and Heat-Purging Mixture (BHM) combined with electro-acupuncture (EA) for peritonitis after operative.In admission order, 32 cases of acute diffuse peritonitis were allocated to group A and were treated with retention-enema of BHM and EA based on routine western medicine 6 hours after operation.Other 28 cases in group B were treated with routine western medicine only. Recovery time of bowel sound,passing flatus and defecation was observed after operation as well as post-operative complications, multiple organs dysfunction syndrome (MODS) and multiple organ failure (MOF).After operation, recovery time of bowel sounding,passing flatus and defecation was 20.32?4.34, 29.43?9.66 and 40.69?13.69 hours in group A respectively, which differed from 30.66?6.06, 40.66?17.33 and 66.48?17.58 hours in group B (P
2.Therapeutic Effect of Tonglieshu capsules for Nonbacterial Prostatitis
Fabin XU ; Yong FANG ; Hongjie LI ; Jianxing XIE
Journal of Guangzhou University of Traditional Chinese Medicine 2004;0(06):-
[ Objective ] To investigate the therapeutic effect of Tonglieshu capsules (mainly composed of Radix Linderae, Semen Litchi, Semen Vaccariae, Rhizoma Corydalis, Rhizoma Sparganii, Rhizoma Curcumae, Herba Taraxaci, etc. ) for nonbacterial prostatitis. [Methods] Forty SD rats were randomized to 4 groups: normal control group (group A) , model group (group B), Qianlietong group (group C) and Tonglieshu, Capsules (group D). Except those in group A, rats in other groups were injected with Xiaozhiling Injection directly into the prostatic glands to establish nonbacterial prostatitis. After treatment with Tonglieshu capsules for 4 weeks, white blood cells counting, density of lecithin corpuscle and inflammatory cells infiltrating into interstitium and glandular lumen of prostatic tissues were observed. [Results] WBC counting was decreased and density of lecithin corpuscle increased in group D (P 0.05) . [Conclusion] Tonglieshu capsules has an effect in treating chronic prostatitis by decreasing the WBC counting, reducing infiltration of inflammatory cells and increasing the density of lecithin corpuscle in prostatic tissues.
3.Inlfuence of age on perioperative clinical parameters, tumor resection effects and complications of patients with early stage gastric cancer for single lesion treated by ESD
Qingfa CHEN ; Yan XU ; Fangxi XUE ; Xingtian CHEN ; Ruiwu WANG ; Fabin WANG
China Journal of Endoscopy 2017;23(2):53-57
Objective To investigate the influence of age on perioperative clinical parameters, tumor resection effects and complications of patients with early stage gastric cancer for single lesion treated by ESD.Methods 60 patients with early stage gastric cancer for single lesion treated by ESD were chosen from January 2013 to July 2014 and divided into 2 groups including A group (35 patients) with < 60 years old and B group (25 patients) with ≥ 60 years old; and the operation time, intraoperative blood loss, hospital staying time, tumor curative resection and completed rate, the recurrence rate with follow-up and complications incidence of 2 groups were compared.Results All the patients were successfully performed the operation and no bleeding cases. There was no significant difference in the operation time, intraoperative blood loss, hospital staying time between 2 groups (P < 0.05). There was no significant difference in the tumor curative resection and completed rate between the 2 groups (P < 0.05). There was no significant difference in the recurrence rate with follow-up between 2 groups (P < 0.05). There was no signiifcant difference in the complications incidence between 2 groups (P < 0.05).Conclusion ESD is safe and feasible in the treatment of elderly patients with early stage gastric cancer for single lesion, and it is equivalent to short- and long-term efifcacy in non-elderly patients and not increase the operation time, the trauma degree and complications risk.
4.Tracking of Stem Cells from Human Exfoliated Deciduous Teeth Labeled with Molday ION Rhodamine-B during Periodontal Bone Regeneration in Rats
Nan ZHANG ; Li XU ; Hao SONG ; Chunqing BU ; Jie KANG ; Chuanchen ZHANG ; Xiaofei YANG ; Fabin HAN
International Journal of Stem Cells 2023;16(1):93-107
Background and Objectives:
Chronic periodontitis can lead to alveolar bone resorption and eventually tooth loss. Stem cells from exfoliated deciduous teeth (SHED) are appropriate bone regeneration seed cells. To track the survival, migration, and differentiation of the transplanted SHED, we used super paramagnetic iron oxide particles (SPIO) Molday ION Rhodamine-B (MIRB) to label and monitor the transplanted cells while repairing periodontal bone defects.
Methods:
and Results: We determined an appropriate dose of MIRB for labeling SHED by examining the growth and osteogenic differentiation of labeled SHED. Finally, SHED was labeled with 25 μg Fe/ml MIRB before being transplanted into rats. Magnetic resonance imaging was used to track SHED survival and migration in vivo due to a low-intensity signal artifact caused by MIRB. HE and immunohistochemical analyses revealed that both MIRB-labeled and unlabeled SHED could promote periodontal bone regeneration. The colocalization of hNUC and MIRB demonstrated that SHED transplanted into rats could survive in vivo. Furthermore, some MIRB-positive cells expressed the osteoblast and osteocyte markers OCN and DMP1, respectively. Enzyme-linked immunosorbent assay revealed that SHED could secrete protein factors, such as IGF-1, OCN, ALP, IL-4, VEGF, and bFGF, which promote bone regeneration. Immunofluorescence staining revealed that the transplanted SHED was surrounded by a large number of host-derived Runx2- and Col II-positive cells that played important roles in the bone healing process.
Conclusions
SHED could promote periodontal bone regeneration in rats, and the survival of SHED could be tracked in vivo by labeling them with MIRB. SHED are likely to promote bone healing through both direct differentiation and paracrine mechanisms.