1.Pneumococcal vaccine promotes the proliferation of CRTH2 (CD4+CD294+ Th2)cells
qin Mei TANG ; qing Fa TIAN ; heng Ju LI ; qing Hui LI ; hui Xiao CHENG
Chinese Journal of Infection Control 2017;16(10):916-919,930
Objective To study the proliferation of CRTH2 (CD4+ CD294+ Th2)cells promoted by pneumococcal vaccine through antigen presentation of dendritic cells (DCs),so as to provide a new approach for amplification and sorting of Th2 cells.Methods CDs induced from peripheral blood mononuclear cells were cocultured with T lym-phocytes after loading pneumococcal vaccine antigen,mixed lymphocyte reaction (MLR)was detected by cell count-ing kit-8(CCK8),DCs and CRTH2 cells were analyzed by flow cytometry.Results Pneumococcal vaccine could promote the maturation of DCs,together with TNF-a,it was adjuvant for maturation of DCs.Pneumococcal vaccine antigen-loaded DCs could increase the rate of subsets of CRTH2 cells on day 5([0.93±0.10]%)compared with day 1([0.70±0.02]%),and absolute number also increased (both P <0.05).Conclusion Amplification of CRTH2 cells can be greatly promoted by pneumococcal vaccine antigen-loaded DCs,which might be one of the effective way to induce amplification of Th2 cells.
2.Epidemiological characteristics of injury caused by fall in the elderly aged ≥60 years in Guang- zhou in 2014-2018
Wei-quan LIU ; Hui LIN ; Yao-hui LI ; Ting-yuan YANG ; Min-ying SUN ; Fa-ju QIN ; Lan LIU ; Ji-chuan SHEN ; Hua-zhang LIU
Chinese Journal of Disease Control & Prevention 2020;24(3):269-273
Objective To explore the epidemiological characteristics and influencing factors of fall of elderly aged ≥ 60 years in Guangzhou from 2014 to 2018 ,so as to provide evidence for effective prevention and control measures. Methods Data on fall of the elderly was collected from the first diagno- sis in injury surveillance hospitals in Guangzhou from 2014 to 2018,and distribution description and epi- demiological analysis were adopted. Results 9 503 cases of fall of the elderly were reported in 5 injury surveillance hospitals,accounting for 49.41% of injuries in the elderly,and had occupied the first place in the cause of injury in 5 consecutive years. The sex ratio of men to women was 1 ∶ 1.67. Most of the patients had a primary or junior school degree,high rate in October-December,mainly occurred at home when doing leisure activities or life activities. The fall mainly led to injuries of head or lower extremities, contributed to contusion /bruise or fracture. And most of them were mild and moderate injury. Logistic re- gression showed that female,old age,high educational level,December to February,at home,walking, leisure activities and life activities were risk factors for falling among the elderly in Guangzhou ( all P < 0.001) . Conclusions Fall is the primary cause of injuries to the elderly in Guangzhou,especially the elderly female population. The targeted prevention and intervention measures should be developed ac- cording to their distribution characteristics.
3.Dendritic Cells Promote the Proliferation of Peripheral Blood CRTH2 Cells (CD4(+)CD294(+)Th2) and Help B Cells to Secrete Immunoglobulin.
Fa-Qing TIAN ; Juan LI ; Ju-Heng LI ; Mei-Qin TANG ; Xiao-Hui CHENG ; Ying-Cai HUANG ; Hui-Qing LI
Journal of Experimental Hematology 2016;24(4):1163-1167
Objective:To investigate the promotive effect of dendritic cells(DCs) on proliferation of CRTH2 (CD4(+)CD294(+)Th2) cells and the influence of CRTH2 cells on secretion of immunoglobulin from B cells so as to provide a new approach for amplification and sorting of Th2 cells. Methods:DCs were induced from peripheral blood mononuclear cells, then the loaded-BCGV-Ag-DCs were cocultured with T cells, and the mixed lymphocyte reaction(MLR) was performed by CCK8 method. The phenotypes of DCs and CRTH2 cells were detected by flow cytometry. CRTH2 cells sorted by MACS were co-cultured with B cells for 5 days to detect the secretion of immunoglobulin. Results:The subsets and absolute number CRTH2 cells were significantly increased by loaded-BCGV-Ag-DCs. The levels of IgG, IgA and IgE were higher increased in supernatant of CRTH2 and B cell co-culture system than that in control group or that in transwell group(P<0.05). Conclusion:The proliferation of CRTH2 cells can be greatly promoted by loaded-BCGV-Ag-DCs, and the CRTH2 cells can help B cells to secrete IgG, IgA and IgE.
B-Lymphocytes
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Cell Proliferation
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Coculture Techniques
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Dendritic Cells
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Flow Cytometry
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Humans
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Immunoglobulins
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Lymphocyte Culture Test, Mixed
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Th2 Cells
4.Analysis of Gene Mutation Types of Thalassemia in Fuzhou Area of China.
Peng-Ju CAO ; Liang-Yuan CHEN ; Li-Li JIANG ; Yang YANG ; Shao-Ting CHEN ; Chun-Li HUANG ; Qiu-Qin ZHANG ; Qiu-Mei WU ; Fa-Lin CHEN
Journal of Experimental Hematology 2019;27(3):893-898
OBJECTIVE:
To investigate the gene mutation types and spectrum of α, β-thalassemia in Fuzhou area of China.
METHODS:
Thalassemia gene screening was performed in the women receiving physical, prenatal, and pre-pregnancy examination, and the patients with suspected thalassemia in our hospital from July 2013 to March 2018.Genotypes of thalassem were detected by Gap-PCR and RDB-PCR.
RESULTS:
1042 were positive among 2074 suspected cases with a positive rate of 50.24%; 618 cases were confirmed to be α-thalassemia and with a positive rate of 29.8%; 409 cases were confirmed to be β-thalassemia with a positive rate of 19.72%. 15 cases were confirmed to be α-β complex thalassemia with a positive rate of 0.72%. the --/αα(76.54%) was the most common genotype among α-thalassemia, -α/αα(10.03%) and -α/αα(2.91%) in hot pursuit. In addition, IVS-II-55 (T->G) and IVS-II-119 (-G, +CTCGGCCC) were newly found alpha mutations; the IVS-2-654 (C→T) (40.83%) was the most common genotype among β-thalassemia, CD41-42 (-TCTT) (35.94%) and CD17 (A→T) (9.78%) in hot pursuit.
CONCLUSION
The genotype of thalassemia in Fuzhou area is highly heterogenic, --/αα is the most common genotype among α-thalassemia, IVS-2-654 (C→T) is the most common genotype among β-thalassemia, Meanwhile, two α-mutation sites are found in this study which were not reported in the Database of Human Hemoglobin Variants and Thalassemias.
China
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Female
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Genotype
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Humans
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Mutation
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Pregnancy
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alpha-Thalassemia
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beta-Thalassemia
5.Terpenoids from Stems and Leaves of Aphanamixis grandifolia
Yan-wu CHEN ; Fa-wu DONG ; Tian-li QIN ; Fan ZHANG ; Shi-li WU ; Miao-yan YANG ; Ju YANG ; Hong-ping HE
Chinese Journal of Experimental Traditional Medical Formulae 2020;26(13):168-173
Objective: